ID   APAH_SALTY              Reviewed;         282 AA.
AC   P0A1B1; Q56018;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Bis(5'-nucleosyl)-tetraphosphatase, symmetrical;
DE            EC=3.6.1.41;
DE   AltName: Full=Ap4A hydrolase;
DE   AltName: Full=Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase;
DE   AltName: Full=Diadenosine tetraphosphatase;
GN   Name=apaH; OrderedLocusNames=STM0088;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RA   Smith R.L., Ahuja D., Maguire M.E.;
RL   Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- FUNCTION: Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield
CC       ADP. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = 2 ADP + 2
CC         H(+); Xref=Rhea:RHEA:24252, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58141, ChEBI:CHEBI:456216; EC=3.6.1.41;
CC   -!- SIMILARITY: Belongs to the Ap4A hydrolase family. {ECO:0000305}.
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DR   EMBL; U24176; AAA79340.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL19052.1; -; Genomic_DNA.
DR   RefSeq; NP_459093.1; NC_003197.2.
DR   RefSeq; WP_000257211.1; NC_003197.2.
DR   AlphaFoldDB; P0A1B1; -.
DR   SMR; P0A1B1; -.
DR   STRING; 99287.STM0088; -.
DR   PaxDb; P0A1B1; -.
DR   EnsemblBacteria; AAL19052; AAL19052; STM0088.
DR   GeneID; 1251606; -.
DR   KEGG; stm:STM0088; -.
DR   PATRIC; fig|99287.12.peg.91; -.
DR   HOGENOM; CLU_056184_2_0_6; -.
DR   OMA; INAFTRM; -.
DR   PhylomeDB; P0A1B1; -.
DR   BioCyc; SENT99287:STM0088-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0008803; F:bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity; IBA:GO_Central.
DR   GO; GO:0016791; F:phosphatase activity; IBA:GO_Central.
DR   GO; GO:0110154; P:RNA decapping; IBA:GO_Central.
DR   CDD; cd07422; MPP_ApaH; 1.
DR   Gene3D; 3.60.21.10; -; 1.
DR   HAMAP; MF_00199; ApaH; 1.
DR   InterPro; IPR004617; ApaH.
DR   InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR   InterPro; IPR029052; Metallo-depent_PP-like.
DR   Pfam; PF00149; Metallophos; 1.
DR   PIRSF; PIRSF000903; B5n-ttraPtase_sm; 1.
DR   SUPFAM; SSF56300; SSF56300; 1.
DR   TIGRFAMs; TIGR00668; apaH; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..282
FT                   /note="Bis(5'-nucleosyl)-tetraphosphatase, symmetrical"
FT                   /id="PRO_0000198009"
FT   CONFLICT        110
FT                   /note="V -> I (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        168
FT                   /note="S -> R (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        171
FT                   /note="A -> G (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        185
FT                   /note="Y -> F (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        198
FT                   /note="A -> E (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        202
FT                   /note="N -> E (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        218..220
FT                   /note="SEA -> AEE (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        252
FT                   /note="E -> T (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        271..282
FT                   /note="QMDMGEGEAVNA -> HKDLGEAAAS (in Ref. 1; AAA79340)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   282 AA;  31431 MW;  B5B701E39CA7869E CRC64;
     MATYLIGDVH GCYDELIALL QQVEFTPDTD TLWLTGDLVA RGPGSLDVLR YVKSLGNSVR
     LVLGNHDLHL LAVFAGISRN KPKDRLTPLL EAPDADELLN WLRRQPLLQV DEEKKLVMAH
     AGITPQWDLQ TAKECARDVE AVLSSDSYPF FLDAMYGDMP NNWSPELSGL ARLRFITNAF
     TRMRYCFPNG QLDMYSKASP ENAPAPLKPW FAIPGPVSEA YSIAFGHWAS LEGKGTPEGI
     YALDTGCCWG GELTCLRWED KQYFVQPSNR QMDMGEGEAV NA