HYBB_ECOLI
ID HYBB_ECOLI Reviewed; 392 AA.
AC P37180; Q2M9K0;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Probable Ni/Fe-hydrogenase 2 b-type cytochrome subunit;
GN Name=hybB; OrderedLocusNames=b2995, JW5494;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / TG1;
RX PubMed=8021226; DOI=10.1128/jb.176.14.4416-4423.1994;
RA Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T.,
RA Peck H.D. Jr., Przybyla A.E.;
RT "Cloning, sequencing, and mutational analysis of the hyb operon encoding
RT Escherichia coli hydrogenase 2.";
RL J. Bacteriol. 176:4416-4423(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Probable b-type cytochrome.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the NrfD family. {ECO:0000305}.
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DR EMBL; U09177; AAA21590.1; -; Genomic_DNA.
DR EMBL; U28377; AAA69162.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U00096; AAC76031.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77056.1; -; Genomic_DNA.
DR PIR; A65086; A65086.
DR RefSeq; NP_417469.1; NC_000913.3.
DR RefSeq; WP_000017703.1; NZ_STEB01000001.1.
DR AlphaFoldDB; P37180; -.
DR SMR; P37180; -.
DR BioGRID; 4262376; 6.
DR ComplexPortal; CPX-282; Hydrogenase-2 complex.
DR STRING; 511145.b2995; -.
DR PaxDb; P37180; -.
DR PRIDE; P37180; -.
DR EnsemblBacteria; AAC76031; AAC76031; b2995.
DR EnsemblBacteria; BAE77056; BAE77056; BAE77056.
DR GeneID; 948615; -.
DR KEGG; ecj:JW5494; -.
DR KEGG; eco:b2995; -.
DR PATRIC; fig|1411691.4.peg.3734; -.
DR EchoBASE; EB1748; -.
DR eggNOG; COG5557; Bacteria.
DR HOGENOM; CLU_049007_0_0_6; -.
DR InParanoid; P37180; -.
DR OMA; AIEVCAY; -.
DR PhylomeDB; P37180; -.
DR BioCyc; EcoCyc:EG11800-MON; -.
DR BioCyc; MetaCyc:EG11800-MON; -.
DR PRO; PR:P37180; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0044569; C:[Ni-Fe] hydrogenase complex; IBA:GO_Central.
DR GO; GO:0009326; C:formate dehydrogenase complex; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:ComplexPortal.
DR GO; GO:0036397; F:formate dehydrogenase (quinone) activity; IBA:GO_Central.
DR GO; GO:0033748; F:hydrogenase (acceptor) activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019645; P:anaerobic electron transport chain; IDA:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IDA:ComplexPortal.
DR GO; GO:0015944; P:formate oxidation; IBA:GO_Central.
DR InterPro; IPR005614; NrfD_fam.
DR Pfam; PF03916; NrfD; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Electron transport; Heme; Iron;
KW Membrane; Metal-binding; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..392
FT /note="Probable Ni/Fe-hydrogenase 2 b-type cytochrome
FT subunit"
FT /id="PRO_0000159326"
FT TOPO_DOM 1..11
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..34
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 56..58
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..90
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..133
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..168
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..207
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..249
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 271..281
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..333
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..392
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 306..307
FT /note="KL -> NV (in Ref. 1; AAA21590/AAA69162)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 392 AA; 43602 MW; 8A25CDCB3EDB67FA CRC64;
MSHDPQPLGG KIISKPVMIF GPLIVICMLL IVKRLVFGLG SVSDLNGGFP WGVWIAFDLL
IGTGFACGGW ALAWAVYVFN RGQYHPLVRP ALLASLFGYS LGGLSITIDV GRYWNLPYFY
IPGHFNVNSV LFETAVCMTI YIGVMALEFA PALFERLGWK VSLQRLNKVM FFIIALGALL
PTMHQSSMGS LMISAGYKVH PLWQSYEMLP LFSLLTAFIM GFSIVIFEGS LVQAGLRGNG
PDEKSLFVKL TNTISVLLAI FIVLRFGELI YRDKLSLAFA GDFYSVMFWI EVLLMLFPLV
VLRVAKLRND SRMLFLSALS ALLGCATWRL TYSLVAFNPG GGYAYFPTWE ELLISIGFVA
IEICAYIVLI RLLPILPPLK QNDHNRHEAS KA
