ID   HYBF_ECO57              Reviewed;         113 AA.
AC   Q8XBW1;
DT   28-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Hydrogenase maturation factor HybF {ECO:0000255|HAMAP-Rule:MF_02099};
GN   Name=hybF {ECO:0000255|HAMAP-Rule:MF_02099};
GN   OrderedLocusNames=Z4345, ECs3876;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_02099}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. HybF subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02099}.
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DR   EMBL; AE005174; AAG58128.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37299.1; -; Genomic_DNA.
DR   PIR; D85958; D85958.
DR   PIR; D91113; D91113.
DR   RefSeq; NP_311903.1; NC_002695.1.
DR   RefSeq; WP_000544954.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8XBW1; -.
DR   SMR; Q8XBW1; -.
DR   STRING; 155864.EDL933_4213; -.
DR   EnsemblBacteria; AAG58128; AAG58128; Z4345.
DR   EnsemblBacteria; BAB37299; BAB37299; ECs_3876.
DR   GeneID; 916300; -.
DR   KEGG; ece:Z4345; -.
DR   KEGG; ecs:ECs_3876; -.
DR   PATRIC; fig|386585.9.peg.4043; -.
DR   eggNOG; COG0375; Bacteria.
DR   HOGENOM; CLU_126929_0_0_6; -.
DR   OMA; ILLCPCG; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   HAMAP; MF_02099; HybF_subfam; 1.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR039002; HybF.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   TIGRFAMs; TIGR00100; hypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Reference proteome; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HybF"
FT                   /id="PRO_0000129065"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         3
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         76
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
SQ   SEQUENCE   113 AA;  12683 MW;  E8AA0C4A40CEABF8 CRC64;
     MHELSLCQSA VEIIQRQAEQ HDVKRVTAVW LEIGALSCVE ESAVRFSFEI VCHGTVAQGC
     DLHIVYKPAQ AWCWDCSQVV EVHQHDAQCP LCHGERLRVD TGDSLIVKSI EVE