ID   HYBF_PROVU              Reviewed;         113 AA.
AC   P20926;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Hydrogenase maturation factor HybF {ECO:0000255|HAMAP-Rule:MF_02099};
GN   Name=hybF {ECO:0000255|HAMAP-Rule:MF_02099};
OS   Proteus vulgaris.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Proteus.
OX   NCBI_TaxID=585;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3308458; DOI=10.1111/j.1432-1033.1987.tb13362.x;
RA   Cole S.T.;
RT   "Nucleotide sequence and comparative analysis of the frd operon encoding
RT   the fumarate reductase of Proteus vulgaris. Extensive sequence divergence
RT   of the membrane anchors and absence of an frd-linked ampC cephalosporinase
RT   gene.";
RL   Eur. J. Biochem. 167:481-488(1987).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_02099}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. HybF subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02099}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X06151; CAA29510.1; -; Genomic_DNA.
DR   PIR; S00118; S00118.
DR   AlphaFoldDB; P20926; -.
DR   SMR; P20926; -.
DR   STRING; 585.DR95_2064; -.
DR   eggNOG; COG0375; Bacteria.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   HAMAP; MF_02099; HybF_subfam; 1.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR039002; HybF.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   TIGRFAMs; TIGR00100; hypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HybF"
FT                   /id="PRO_0000129068"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         3
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         76
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
SQ   SEQUENCE   113 AA;  12620 MW;  17C4D773C6A1B2DD CRC64;
     MHEITLCQSA FEIIDSQAKL NNAQKVKSVW MEISALSCVE VSALEFCFDI VCRDTIAQGC
     QLHIEVIPAK AWCWDCHQVV TVSTFNAGCP ACGSQNLRVE NDDAMRIKQI EIE