ID   HYBF_SHIB3              Reviewed;         113 AA.
AC   B2U195;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Hydrogenase maturation factor HybF {ECO:0000255|HAMAP-Rule:MF_02099};
GN   Name=hybF {ECO:0000255|HAMAP-Rule:MF_02099};
GN   OrderedLocusNames=SbBS512_E3418;
OS   Shigella boydii serotype 18 (strain CDC 3083-94 / BS512).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=344609;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 3083-94 / BS512;
RA   Rasko D.A., Rosovitz M., Maurelli A.T., Myers G., Seshadri R., Cer R.,
RA   Jiang L., Ravel J., Sebastian Y.;
RT   "Complete sequence of Shigella boydii serotype 18 strain BS512.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_02099}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. HybF subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_02099}.
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DR   EMBL; CP001063; ACD06820.1; -; Genomic_DNA.
DR   RefSeq; WP_000544953.1; NC_010658.1.
DR   AlphaFoldDB; B2U195; -.
DR   SMR; B2U195; -.
DR   STRING; 344609.SbBS512_E3418; -.
DR   EnsemblBacteria; ACD06820; ACD06820; SbBS512_E3418.
DR   GeneID; 58459877; -.
DR   KEGG; sbc:SbBS512_E3418; -.
DR   HOGENOM; CLU_126929_0_0_6; -.
DR   OMA; ILLCPCG; -.
DR   Proteomes; UP000001030; Chromosome.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   HAMAP; MF_02099; HybF_subfam; 1.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR039002; HybF.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   TIGRFAMs; TIGR00100; hypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HybF"
FT                   /id="PRO_1000099898"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         3
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         76
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02099"
SQ   SEQUENCE   113 AA;  12697 MW;  70919C4A40CEABE6 CRC64;
     MHELSLCQSA VEIIQRQAEQ HDVKRVTAVW LEIGALSCVE ESAVRFSFEI VCHGTVAQGC
     DLHIVYKPAQ AWCWDCSQVV EIHQHDAQCP LCHGERLRVD TGDSLIVKSI EVE