HYBG_ECOLI
ID HYBG_ECOLI Reviewed; 82 AA.
AC P0AAM7; P37185; Q2M9K5;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Hydrogenase maturation factor HybG {ECO:0000305};
DE AltName: Full=Hydrogenase-2 operon protein HybG {ECO:0000305};
GN Name=hybG {ECO:0000303|PubMed:8021226}; OrderedLocusNames=b2990, JW2958;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / TG1;
RX PubMed=8021226; DOI=10.1128/jb.176.14.4416-4423.1994;
RA Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T.,
RA Peck H.D. Jr., Przybyla A.E.;
RT "Cloning, sequencing, and mutational analysis of the hyb operon encoding
RT Escherichia coli hydrogenase 2.";
RL J. Bacteriol. 176:4416-4423(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, INTERACTION WITH HYBC, AND DISRUPTION PHENOTYPE.
RX PubMed=11292801; DOI=10.1128/jb.183.9.2817-2822.2001;
RA Blokesch M., Magalon A., Boeck A.;
RT "Interplay between the specific chaperone-like proteins HybG and HypC in
RT maturation of hydrogenases 1, 2, and 3 from Escherichia coli.";
RL J. Bacteriol. 183:2817-2822(2001).
RN [5]
RP PROBABLE FUNCTION, AND INTERACTION WITH HYPD; HYPE; HYBC AND HYAB.
RX PubMed=16412426; DOI=10.1016/j.febslet.2005.12.063;
RA Butland G., Zhang J.W., Yang W., Sheung A., Wong P., Greenblatt J.F.,
RA Emili A., Zamble D.B.;
RT "Interactions of the Escherichia coli hydrogenase biosynthetic proteins:
RT HybG complex formation.";
RL FEBS Lett. 580:677-681(2006).
RN [6]
RP FUNCTION.
RX PubMed=23851071; DOI=10.1016/j.febslet.2013.06.055;
RA Soboh B., Stripp S.T., Bielak C., Lindenstrauss U., Braussemann M.,
RA Javaid M., Hallensleben M., Granich C., Herzberg M., Heberle J.,
RA Sawers R.G.;
RT "The [NiFe]-hydrogenase accessory chaperones HypC and HybG of Escherichia
RT coli are iron- and carbon dioxide-binding proteins.";
RL FEBS Lett. 587:2512-2516(2013).
CC -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Involved
CC in the biosynthesis of the Fe(CN)(2)CO cofactor (PubMed:16412426). HybG
CC delivers iron-bound CO(2) to HypD where reduction to CO probably occurs
CC (PubMed:23851071). In complex with HypD, accepts the cyanide ligand
CC generated by HypF and HypE, and also coordinates the carbon monoxide
CC ligand (By similarity). Involved in the maturation of the hydrogenase
CC 2. Also participates in the maturation of hydrogenase 1
CC (PubMed:11292801). {ECO:0000250|UniProtKB:P0AAM3,
CC ECO:0000269|PubMed:11292801, ECO:0000269|PubMed:23851071,
CC ECO:0000305|PubMed:16412426}.
CC -!- PATHWAY: Protein modification; [NiFe] hydrogenase maturation.
CC {ECO:0000305}.
CC -!- SUBUNIT: Forms a complex with HypD and HypE (PubMed:16412426).
CC Interacts with the precursor form of HybC (pre-HybC), the large subunit
CC of hydrogenase 2, and with HyaB, the large subunit of hydrogenase 1
CC (PubMed:11292801, PubMed:16412426). {ECO:0000269|PubMed:11292801,
CC ECO:0000269|PubMed:16412426}.
CC -!- INTERACTION:
CC P0AAM7; P0ACE0: hybC; NbExp=2; IntAct=EBI-562426, EBI-549849;
CC P0AAM7; P24192: hypD; NbExp=7; IntAct=EBI-562426, EBI-552711;
CC P0AAM7; P43674: ycaL; NbExp=2; IntAct=EBI-562426, EBI-9128400;
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene abolishes the generation of
CC active hydrogenase 2, but only reduces hydrogenase 1 activity. It does
CC not affect the maturation of hydrogenase 3. Deletion of both hybG and
CC hypC completely abolishes hydrogenase activity.
CC {ECO:0000269|PubMed:11292801}.
CC -!- SIMILARITY: Belongs to the HupF/HypC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U09177; AAA21595.1; -; Genomic_DNA.
DR EMBL; U28377; AAA69157.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76026.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77051.1; -; Genomic_DNA.
DR PIR; G55516; G55516.
DR RefSeq; NP_417464.1; NC_000913.3.
DR RefSeq; WP_000334896.1; NZ_STEB01000001.1.
DR AlphaFoldDB; P0AAM7; -.
DR SMR; P0AAM7; -.
DR BioGRID; 4262372; 7.
DR BioGRID; 852328; 4.
DR ComplexPortal; CPX-5283; Hybg-HypDE Ni-hydrogenase maturation complex.
DR DIP; DIP-36429N; -.
DR IntAct; P0AAM7; 11.
DR STRING; 511145.b2990; -.
DR PaxDb; P0AAM7; -.
DR EnsemblBacteria; AAC76026; AAC76026; b2990.
DR EnsemblBacteria; BAE77051; BAE77051; BAE77051.
DR GeneID; 67415062; -.
DR GeneID; 948020; -.
DR KEGG; ecj:JW2958; -.
DR KEGG; eco:b2990; -.
DR PATRIC; fig|1411691.4.peg.3739; -.
DR EchoBASE; EB1753; -.
DR eggNOG; COG0298; Bacteria.
DR HOGENOM; CLU_159381_1_1_6; -.
DR InParanoid; P0AAM7; -.
DR OMA; HYPARVD; -.
DR PhylomeDB; P0AAM7; -.
DR BioCyc; EcoCyc:EG11805-MON; -.
DR UniPathway; UPA00335; -.
DR PRO; PR:P0AAM7; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1902670; F:carbon dioxide binding; IDA:EcoCyc.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0005506; F:iron ion binding; IDA:EcoCyc.
DR GO; GO:0051604; P:protein maturation; IDA:EcoCyc.
DR InterPro; IPR019812; Hydgase_assmbl_chp_CS.
DR InterPro; IPR001109; Hydrogenase_HupF/HypC.
DR PANTHER; PTHR35177; PTHR35177; 1.
DR Pfam; PF01455; HupF_HypC; 1.
DR PRINTS; PR00445; HUPFHYPC.
DR TIGRFAMs; TIGR00074; hypC_hupF; 1.
DR PROSITE; PS01097; HUPF_HYPC; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..82
FT /note="Hydrogenase maturation factor HybG"
FT /id="PRO_0000201403"
FT SITE 2
FT /note="Important for interaction with HypD and the
FT precursor form of hydrogenase"
FT /evidence="ECO:0000250|UniProtKB:P0AAM3"
SQ SEQUENCE 82 AA; 8808 MW; 8E9736BFC4EFD626 CRC64;
MCIGVPGQVL AVGEDIHQLA QVEVCGIKRD VNIALICEGN PADLLGQWVL VHVGFAMSII
DEDEAKATLD ALRQMDYDIT SA
