HYCA_ECOLI
ID HYCA_ECOLI Reviewed; 153 AA.
AC P0AEV4; P16427; Q2MAA4;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Formate hydrogenlyase regulatory protein HycA;
GN Name=hycA; Synonyms=hevA; OrderedLocusNames=b2725, JW2695;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=2187144; DOI=10.1111/j.1365-2958.1990.tb00590.x;
RA Boehm R., Sauter M., Boeck A.;
RT "Nucleotide sequence and expression of an operon in Escherichia coli coding
RT for formate hydrogenlyase components.";
RL Mol. Microbiol. 4:231-243(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RX PubMed=1625581; DOI=10.1111/j.1365-2958.1992.tb00873.x;
RA Sauter M., Boehm R., Boeck A.;
RT "Mutational analysis of the operon (hyc) determining hydrogenase 3
RT formation in Escherichia coli.";
RL Mol. Microbiol. 6:1523-1532(1992).
RN [5]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
RN [6]
RP FUNCTION, REGULON, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=12426353; DOI=10.1128/jb.184.23.6642-6653.2002;
RA Skibinski D.A., Golby P., Chang Y.S., Sargent F., Hoffman R., Harper R.,
RA Guest J.R., Attwood M.M., Berks B.C., Andrews S.C.;
RT "Regulation of the hydrogenase-4 operon of Escherichia coli by the
RT sigma(54)-dependent transcriptional activators FhlA and HyfR.";
RL J. Bacteriol. 184:6642-6653(2002).
CC -!- FUNCTION: Regulatory protein for the formate hydrogenlyase system.
CC Could act by directly interacting with FhlA or by preventing the
CC binding of FhlA to the upstream activatory sequence (PubMed:1625581).
CC Also down-regulates expression of the hyf operon (PubMed:12426353).
CC {ECO:0000269|PubMed:12426353, ECO:0000269|PubMed:1625581}.
CC -!- DISRUPTION PHENOTYPE: Increased expression of the hyf operon
CC (PubMed:12426353). {ECO:0000269|PubMed:12426353}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X17506; CAA35546.1; -; Genomic_DNA.
DR EMBL; U29579; AAA69235.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75767.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76802.1; -; Genomic_DNA.
DR PIR; S08619; S08619.
DR RefSeq; NP_417205.1; NC_000913.3.
DR RefSeq; WP_000158056.1; NZ_STEB01000027.1.
DR AlphaFoldDB; P0AEV4; -.
DR BioGRID; 4262102; 11.
DR BioGRID; 851525; 1.
DR IntAct; P0AEV4; 1.
DR STRING; 511145.b2725; -.
DR PaxDb; P0AEV4; -.
DR PRIDE; P0AEV4; -.
DR EnsemblBacteria; AAC75767; AAC75767; b2725.
DR EnsemblBacteria; BAE76802; BAE76802; BAE76802.
DR GeneID; 66673406; -.
DR GeneID; 947193; -.
DR KEGG; ecj:JW2695; -.
DR KEGG; eco:b2725; -.
DR PATRIC; fig|1411691.4.peg.4016; -.
DR EchoBASE; EB0469; -.
DR eggNOG; ENOG50305VH; Bacteria.
DR HOGENOM; CLU_115336_0_0_6; -.
DR OMA; HAMSCAA; -.
DR PhylomeDB; P0AEV4; -.
DR BioCyc; EcoCyc:EG10474-MON; -.
DR PRO; PR:P0AEV4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR InterPro; IPR021285; Tscrpt_reg_HycA.
DR Pfam; PF11046; HycA_repressor; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..153
FT /note="Formate hydrogenlyase regulatory protein HycA"
FT /id="PRO_0000084100"
SQ SEQUENCE 153 AA; 17627 MW; CB61EC856450A0AD CRC64;
MTIWEISEKA DYIAQRHRRL QDQWHIYCNS LVQGITLSKA RLHHAMSCAP DKELCFVLFE
HFRIYVTLAD GFNSHTIEYY VETKDGEDKQ RIAQAQLSID GMIDGKVNIR DREQVLEHYL
EKIAGVYDSL YTAIENNVPV NLSQLVKGQS PAA
