HYCC_ECOLI
ID HYCC_ECOLI Reviewed; 608 AA.
AC P16429; Q2MAA6; Q46882;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Formate hydrogenlyase subunit 3;
DE Short=FHL subunit 3;
DE AltName: Full=Hydrogenase-3 component C;
GN Name=hycC; Synonyms=hevC; OrderedLocusNames=b2723, JW2693;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=2187144; DOI=10.1111/j.1365-2958.1990.tb00590.x;
RA Boehm R., Sauter M., Boeck A.;
RT "Nucleotide sequence and expression of an operon in Escherichia coli coding
RT for formate hydrogenlyase components.";
RL Mol. Microbiol. 4:231-243(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- SUBUNIT: FHL comprises of a formate dehydrogenase, unidentified
CC electron carriers and a hydrogenase (isoenzyme 3). In this non-energy
CC conserving pathway molecular hydrogen and carbodioxide from formate are
CC released.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; X17506; CAA35548.1; -; Genomic_DNA.
DR EMBL; U29579; AAA69233.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75765.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76800.1; -; Genomic_DNA.
DR PIR; G65052; G65052.
DR RefSeq; NP_417203.1; NC_000913.3.
DR RefSeq; WP_001274396.1; NZ_LN832404.1.
DR AlphaFoldDB; P16429; -.
DR SMR; P16429; -.
DR BioGRID; 4262104; 8.
DR ComplexPortal; CPX-317; Formate hydrogenlyase-H/Hydrogenase-3 complex.
DR DIP; DIP-9973N; -.
DR IntAct; P16429; 2.
DR STRING; 511145.b2723; -.
DR TCDB; 3.D.1.9.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P16429; -.
DR PRIDE; P16429; -.
DR EnsemblBacteria; AAC75765; AAC75765; b2723.
DR EnsemblBacteria; BAE76800; BAE76800; BAE76800.
DR GeneID; 945327; -.
DR KEGG; ecj:JW2693; -.
DR KEGG; eco:b2723; -.
DR PATRIC; fig|1411691.4.peg.4018; -.
DR EchoBASE; EB0471; -.
DR eggNOG; COG0651; Bacteria.
DR HOGENOM; CLU_007100_8_2_6; -.
DR InParanoid; P16429; -.
DR OMA; PPLWWGV; -.
DR PhylomeDB; P16429; -.
DR BioCyc; EcoCyc:HYCC-MON; -.
DR BioCyc; MetaCyc:HYCC-MON; -.
DR PRO; PR:P16429; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IPI:ComplexPortal.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0019645; P:anaerobic electron transport chain; IDA:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IDA:ComplexPortal.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015944; P:formate oxidation; IDA:ComplexPortal.
DR GO; GO:0006007; P:glucose catabolic process; IDA:ComplexPortal.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..608
FT /note="Formate hydrogenlyase subunit 3"
FT /id="PRO_0000118048"
FT TRANSMEM 10..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..476
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 502..521
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 227
FT /note="A -> T (in Ref. 1; CAA35548)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 608 AA; 64077 MW; 7E4F4C63626289B8 CRC64;
MSAISLINSG VAWFVAAAVL AFLFSFQKAL SGWIAGIGGA VGSLYTAAAG FTVLTGAVGV
SGALSLVSYD VQISPLNAIW LITLGLCGLF VSLYNIDWHR HAQVKCNGLQ INMLMAAAVC
AVIASNLGMF VVMAEIMALC AVFLTSNSKE GKLWFALGRL GTLLLAIACW LLWQRYGTLD
LRLLDMRMQQ LPLGSDIWLL GVIGFGLLAG IIPLHGWVPQ AHANASAPAA ALFSTVVMKI
GLLGILTLSL LGGNAPLWWG IALLVLGMIT AFVGGLYALV EHNIQRLLAY HTLENIGIIL
LGLGAGVTGI ALEQPALIAL GLVGGLYHLL NHSLFKSVLF LGAGSVWFRT GHRDIEKLGG
IGKKMPVISI AMLVGLMAMA ALPPLNGFAG EWVIYQSFFK LSNSGAFVAR LLGPLLAVGL
AITGALAVMC MAKVYGVTFL GAPRTKEAEN ATCAPLLMSV SVVALAICCV IGGVAAPWLL
PMLSAAVPLP LEPANTTVSQ PMITLLLIAC PLLPFIIMAI CKGDRLPSRS RGAAWVCGYD
HEKSMVITAH GFAMPVKQAF APVLKLRKWL NPVSLVPGWQ CEGSALLFRR MALVELAVLV
VIIVSRGA
