HYCD_ECOLI
ID HYCD_ECOLI Reviewed; 307 AA.
AC P16430; Q2MAA7;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Formate hydrogenlyase subunit 4;
DE Short=FHL subunit 4;
DE AltName: Full=Hydrogenase 3 component D;
GN Name=hycD; Synonyms=hevD; OrderedLocusNames=b2722, JW2692;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=2187144; DOI=10.1111/j.1365-2958.1990.tb00590.x;
RA Boehm R., Sauter M., Boeck A.;
RT "Nucleotide sequence and expression of an operon in Escherichia coli coding
RT for formate hydrogenlyase components.";
RL Mol. Microbiol. 4:231-243(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- SUBUNIT: FHL comprises of a formate dehydrogenase, unidentified
CC electron carriers and a hydrogenase (isoenzyme 3). In this non-energy
CC conserving pathway molecular hydrogen and carbodioxide from formate are
CC released.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X17506; CAA35549.1; -; Genomic_DNA.
DR EMBL; U29579; AAA69232.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75764.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76799.1; -; Genomic_DNA.
DR PIR; S08622; S08622.
DR RefSeq; NP_417202.1; NC_000913.3.
DR RefSeq; WP_000115224.1; NZ_LN832404.1.
DR AlphaFoldDB; P16430; -.
DR SMR; P16430; -.
DR BioGRID; 4261429; 6.
DR ComplexPortal; CPX-317; Formate hydrogenlyase-H/Hydrogenase-3 complex.
DR IntAct; P16430; 1.
DR STRING; 511145.b2722; -.
DR TCDB; 3.D.1.9.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR jPOST; P16430; -.
DR PaxDb; P16430; -.
DR PRIDE; P16430; -.
DR EnsemblBacteria; AAC75764; AAC75764; b2722.
DR EnsemblBacteria; BAE76799; BAE76799; BAE76799.
DR GeneID; 948994; -.
DR KEGG; ecj:JW2692; -.
DR KEGG; eco:b2722; -.
DR PATRIC; fig|1411691.4.peg.4019; -.
DR EchoBASE; EB0472; -.
DR eggNOG; COG0650; Bacteria.
DR HOGENOM; CLU_015134_2_1_6; -.
DR InParanoid; P16430; -.
DR OMA; KIMARIQ; -.
DR PhylomeDB; P16430; -.
DR BioCyc; EcoCyc:HYCD-MON; -.
DR BioCyc; MetaCyc:HYCD-MON; -.
DR PRO; PR:P16430; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IPI:ComplexPortal.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0019645; P:anaerobic electron transport chain; IDA:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IDA:ComplexPortal.
DR GO; GO:0015944; P:formate oxidation; IDA:ComplexPortal.
DR GO; GO:0006007; P:glucose catabolic process; IDA:ComplexPortal.
DR InterPro; IPR001694; NADH_UbQ_OxRdtase_su1/FPO.
DR InterPro; IPR018086; NADH_UbQ_OxRdtase_su1_CS.
DR Pfam; PF00146; NADHdh; 1.
DR PROSITE; PS00667; COMPLEX1_ND1_1; 1.
DR PROSITE; PS00668; COMPLEX1_ND1_2; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..307
FT /note="Formate hydrogenlyase subunit 4"
FT /id="PRO_0000117534"
FT TOPO_DOM 1..2
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..67
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..93
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..167
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..253
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..284
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..307
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 307 AA; 33029 MW; 2A3F410620E82AC9 CRC64;
MSVLYPLIQA LVLFAVAPLL SGITRVARAR LHNRRGPGVL QEYRDIIKLL GRQSVGPDAS
GWVFRLTPYV MVGVMLTIAT ALPVVTVGSP LPQLGDLITL LYLFAIARFF FAISGLDTGS
PFTAIGASRE AMLGVLVEPM LLLGLWVAAQ VAGSTNISNI TDTVYHWPLS QSIPLVLALC
ACAFATFIEM GKLPFDLAEA EQELQEGPLS EYSGSGFGVM KWGISLKQLV VLQMFVGVFI
PWGQMETFTA GGLLLALVIA IVKLVVGVLV IALFENSMAR LRLDITPRIT WAGFGFAFLA
FVSLLAA
