HYCE_ECOLI
ID HYCE_ECOLI Reviewed; 569 AA.
AC P16431; Q2MAA8;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Formate hydrogenlyase subunit 5;
DE Short=FHL subunit 5;
DE AltName: Full=Hydrogenase-3 component E;
DE Flags: Precursor;
GN Name=hycE; Synonyms=hevE; OrderedLocusNames=b2721, JW2691;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=2187144; DOI=10.1111/j.1365-2958.1990.tb00590.x;
RA Boehm R., Sauter M., Boeck A.;
RT "Nucleotide sequence and expression of an operon in Escherichia coli coding
RT for formate hydrogenlyase components.";
RL Mol. Microbiol. 4:231-243(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROTEOLYTIC PROCESSING.
RX PubMed=8125094; DOI=10.1111/j.1432-1033.1994.tb18634.x;
RA Rossmann R., Sauter M., Lottspeich F., Boeck A.;
RT "Maturation of the large subunit (HYCE) of Escherichia coli hydrogenase 3
RT requires nickel incorporation followed by C-terminal processing at
RT Arg537.";
RL Eur. J. Biochem. 220:377-384(1994).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000305};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000305};
CC -!- SUBUNIT: FHL comprises of a formate dehydrogenase, unidentified
CC electron carriers and a hydrogenase (isoenzyme 3). In this non-energy
CC conserving pathway molecular hydrogen and carbodioxide from formate are
CC released.
CC -!- INTERACTION:
CC P16431; P0A700: hypA; NbExp=14; IntAct=EBI-552702, EBI-6290024;
CC P16431; P0A9K9: slyD; NbExp=7; IntAct=EBI-552702, EBI-369251;
CC -!- SIMILARITY: Belongs to the complex I 49 kDa subunit family.
CC {ECO:0000305}.
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DR EMBL; X17506; CAA35550.1; -; Genomic_DNA.
DR EMBL; U29579; AAA69231.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75763.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76798.1; -; Genomic_DNA.
DR PIR; S08623; S08623.
DR RefSeq; NP_417201.1; NC_000913.3.
DR RefSeq; WP_001288122.1; NZ_SSUR01000024.1.
DR AlphaFoldDB; P16431; -.
DR SMR; P16431; -.
DR BioGRID; 4261447; 11.
DR BioGRID; 851718; 2.
DR ComplexPortal; CPX-317; Formate hydrogenlyase-H/Hydrogenase-3 complex.
DR DIP; DIP-9975N; -.
DR IntAct; P16431; 18.
DR MINT; P16431; -.
DR STRING; 511145.b2721; -.
DR TCDB; 3.D.1.9.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P16431; -.
DR PRIDE; P16431; -.
DR EnsemblBacteria; AAC75763; AAC75763; b2721.
DR EnsemblBacteria; BAE76798; BAE76798; BAE76798.
DR GeneID; 947396; -.
DR KEGG; ecj:JW2691; -.
DR KEGG; eco:b2721; -.
DR PATRIC; fig|1411691.4.peg.4020; -.
DR EchoBASE; EB0473; -.
DR eggNOG; COG3261; Bacteria.
DR eggNOG; COG3262; Bacteria.
DR HOGENOM; CLU_015134_3_1_6; -.
DR InParanoid; P16431; -.
DR OMA; CDPCFTC; -.
DR PhylomeDB; P16431; -.
DR BioCyc; EcoCyc:HYCELARGE-MON; -.
DR BioCyc; MetaCyc:HYCELARGE-MON; -.
DR PRO; PR:P16431; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IPI:ComplexPortal.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IDA:EcoCyc.
DR GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR GO; GO:0019645; P:anaerobic electron transport chain; IDA:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IDA:ComplexPortal.
DR GO; GO:0015944; P:formate oxidation; IDA:ComplexPortal.
DR GO; GO:0006007; P:glucose catabolic process; IDA:ComplexPortal.
DR Gene3D; 1.10.645.10; -; 1.
DR Gene3D; 3.30.460.80; -; 1.
DR InterPro; IPR001135; NADH_Q_OxRdtase_suD.
DR InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR InterPro; IPR014029; NADH_UbQ_OxRdtase_49kDa_CS.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR029014; NiFe-Hase_large.
DR Pfam; PF00329; Complex1_30kDa; 1.
DR Pfam; PF00346; Complex1_49kDa; 2.
DR Pfam; PF00374; NiFeSe_Hases; 1.
DR SUPFAM; SSF143243; SSF143243; 1.
DR SUPFAM; SSF56762; SSF56762; 1.
DR PROSITE; PS00535; COMPLEX1_49K; 1.
PE 1: Evidence at protein level;
KW 4Fe-4S; Iron; Iron-sulfur; Metal-binding; NAD; Nickel; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..537
FT /note="Formate hydrogenlyase subunit 5"
FT /id="PRO_0000019985"
FT PROPEP 538..569
FT /id="PRO_0000019986"
SQ SEQUENCE 569 AA; 64980 MW; 38A2BCCB01B27463 CRC64;
MSEEKLGQHY LAALNEAFPG VVLDHAWQTK DQLTVTVKVN YLPEVVEFLY YKQGGWLSVL
FGNDERKLNG HYAVYYVLSM EKGTKCWITV RVEVDANKPE YPSVTPRVPA AVWGEREVRD
MYGLIPVGLP DERRLVLPDD WPDELYPLRK DSMDYRQRPA PTTDAETYEF INELGDKKNN
VVPIGPLHVT SDEPGHFRLF VDGENIIDAD YRLFYVHRGM EKLAETRMGY NEVTFLSDRV
CGICGFAHST AYTTSVENAM GIQVPERAQM IRAILLEVER LHSHLLNLGL ACHFTGFDSG
FMQFFRVRET SMKMAEILTG ARKTYGLNLI GGIRRDLLKD DMIQTRQLAQ QMRREVQELV
DVLLSTPNME QRTVGIGRLD PEIARDFSNV GPMVRASGHA RDTRADHPFV GYGLLPMEVH
SEQGCDVISR LKVRINEVYT ALNMIDYGLD NLPGGPLMVE GFTYIPHRFA LGFAEAPRGD
DIHWSMTGDN QKLYRWRCRA ATYANWPTLR YMLRGNTVSD APLIIGSLDP CYSCTDRMTV
VDVRKKKSKV VPYKELERYS IERKNSPLK
