HYDIN_MOUSE
ID HYDIN_MOUSE Reviewed; 5154 AA.
AC Q80W93; Q3UQ06; Q68ED1; Q8BRL9; Q8CDF4;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Hydrocephalus-inducing protein;
DE AltName: Full=Protein Hy-3;
GN Name=Hydin; Synonyms=Hy3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DISEASE, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J;
RX PubMed=12719380; DOI=10.1093/hmg/ddg122;
RA Davy B.E., Robinson M.L.;
RT "Congenital hydrocephalus in hy3 mice is caused by a frameshift mutation in
RT Hydin, a large novel gene.";
RL Hum. Mol. Genet. 12:1163-1170(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 1-113 AND 4193-5154 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain cortex, Lung, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4014-5154 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP REVIEW.
RX PubMed=17296793; DOI=10.1083/jcb.200701113;
RA Smith E.F.;
RT "Hydin seek: finding a function in ciliary motility.";
RL J. Cell Biol. 176:403-404(2007).
RN [5]
RP FUNCTION, AND DISEASE.
RX PubMed=18250199; DOI=10.1083/jcb.200710162;
RA Lechtreck K.F., Delmotte P., Robinson M.L., Sanderson M.J., Witman G.B.;
RT "Mutations in Hydin impair ciliary motility in mice.";
RL J. Cell Biol. 180:633-643(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=30535028; DOI=10.1093/jmcb/mjy014;
RA Zheng J., Liu H., Zhu L., Chen Y., Zhao H., Zhang W., Li F., Xie L.,
RA Yan X., Zhu X.;
RT "Microtubule-bundling protein Spef1 enables mammalian ciliary central
RT apparatus formation.";
RL J. Mol. Cell Biol. 11:67-77(2019).
RN [8]
RP INTERACTION WITH KIF9.
RX PubMed=32072696; DOI=10.1096/fj.201902755r;
RA Miyata H., Shimada K., Morohoshi A., Oura S., Matsumura T., Xu Z.,
RA Oyama Y., Ikawa M.;
RT "Testis-enriched kinesin KIF9 is important for progressive motility in
RT mouse spermatozoa.";
RL FASEB J. 34:5389-5400(2020).
CC -!- FUNCTION: Required for ciliary motility. {ECO:0000269|PubMed:18250199}.
CC -!- SUBUNIT: Interacts with KIF9. {ECO:0000269|PubMed:32072696}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000305}. Cytoplasm,
CC cytoskeleton, cilium axoneme {ECO:0000269|PubMed:30535028}.
CC Note=Localizes in the cilium axoneme in a SPEF1-dependent manner.
CC {ECO:0000269|PubMed:30535028}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q80W93-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80W93-2; Sequence=VSP_024701, VSP_024702;
CC Name=3;
CC IsoId=Q80W93-3; Sequence=VSP_040915, VSP_040916, VSP_040917;
CC -!- TISSUE SPECIFICITY: Expressed in brain and testis. Expressed in
CC ciliated epithelial cells lining bronchi and oviduct, and in
CC spermatocytes. {ECO:0000269|PubMed:12719380}.
CC -!- DEVELOPMENTAL STAGE: At 15 dpc, expressed in developing choroid plexus.
CC At P0, brain expression is limited to the ciliated ependymal cells.
CC {ECO:0000269|PubMed:12719380}.
CC -!- DISEASE: Note=Defects in Hydin are the cause of spontaneous
CC hydrocephalus 3, a lethal communicating hydrocephalus with perinatal
CC onset. There is lethality in the first weeks of life because of
CC hydrocephalus caused by abnormal ependymal ciliary motility. Cilia are
CC unable to bend normally, ciliary beat frequency is reduced, and the
CC cilia tend to stall. As a result, these cilia are incapable of
CC generating fluid flow. Similar defects are observed for cilia in
CC trachea. Mice do not exhibit randomization of left-right body asymmetry
CC or situs inversus. {ECO:0000269|PubMed:12719380,
CC ECO:0000269|PubMed:18250199}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-56 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO44953.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC26807.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BB664150; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the C-terminal part.; Evidence={ECO:0000305};
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DR EMBL; AY173049; AAO44953.1; ALT_INIT; mRNA.
DR EMBL; AK030144; BAC26807.1; ALT_INIT; mRNA.
DR EMBL; AK043971; BAC31720.1; -; mRNA.
DR EMBL; AK142981; BAE25238.1; -; mRNA.
DR EMBL; BB641870; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BB664150; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC080316; AAH80316.1; -; mRNA.
DR CCDS; CCDS40476.1; -. [Q80W93-1]
DR RefSeq; NP_766504.3; NM_172916.2. [Q80W93-1]
DR SMR; Q80W93; -.
DR BioGRID; 232672; 4.
DR STRING; 10090.ENSMUSP00000046204; -.
DR iPTMnet; Q80W93; -.
DR PhosphoSitePlus; Q80W93; -.
DR MaxQB; Q80W93; -.
DR PaxDb; Q80W93; -.
DR PRIDE; Q80W93; -.
DR ProteomicsDB; 273238; -. [Q80W93-1]
DR ProteomicsDB; 273239; -. [Q80W93-2]
DR ProteomicsDB; 273240; -. [Q80W93-3]
DR Antibodypedia; 66504; 48 antibodies from 12 providers.
DR DNASU; 244653; -.
DR Ensembl; ENSMUST00000043141; ENSMUSP00000046204; ENSMUSG00000059854. [Q80W93-1]
DR GeneID; 244653; -.
DR KEGG; mmu:244653; -.
DR UCSC; uc009nkq.1; mouse. [Q80W93-3]
DR UCSC; uc009nkr.1; mouse. [Q80W93-2]
DR UCSC; uc009nks.1; mouse. [Q80W93-1]
DR CTD; 54768; -.
DR MGI; MGI:2389007; Hydin.
DR VEuPathDB; HostDB:ENSMUSG00000059854; -.
DR eggNOG; ENOG502QQ4F; Eukaryota.
DR GeneTree; ENSGT00610000086095; -.
DR HOGENOM; CLU_000116_1_0_1; -.
DR InParanoid; Q80W93; -.
DR OMA; AHLQMEV; -.
DR OrthoDB; 3322at2759; -.
DR PhylomeDB; Q80W93; -.
DR TreeFam; TF328687; -.
DR BioGRID-ORCS; 244653; 5 hits in 71 CRISPR screens.
DR ChiTaRS; Hydin; mouse.
DR PRO; PR:Q80W93; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q80W93; protein.
DR Bgee; ENSMUSG00000059854; Expressed in spermatid and 26 other tissues.
DR Genevisible; Q80W93; MM.
DR GO; GO:1990716; C:axonemal central apparatus; IDA:UniProtKB.
DR GO; GO:1990718; C:axonemal central pair projection; IMP:MGI.
DR GO; GO:0005930; C:axoneme; IBA:GO_Central.
DR GO; GO:1904158; P:axonemal central apparatus assembly; IMP:MGI.
DR GO; GO:0007420; P:brain development; IMP:MGI.
DR GO; GO:0003341; P:cilium movement; IMP:MGI.
DR GO; GO:0002064; P:epithelial cell development; IMP:MGI.
DR GO; GO:0060438; P:trachea development; IMP:MGI.
DR GO; GO:0021591; P:ventricular system development; IMP:MGI.
DR Gene3D; 2.60.40.10; -; 22.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031549; ASH.
DR InterPro; IPR033305; Hydin-like.
DR InterPro; IPR033768; Hydin_ADK.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008962; PapD-like_sf.
DR PANTHER; PTHR23053; PTHR23053; 1.
DR Pfam; PF15780; ASH; 1.
DR Pfam; PF17213; Hydin_ADK; 1.
DR SUPFAM; SSF49354; SSF49354; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Coiled coil; Cytoplasm;
KW Cytoskeleton; Reference proteome.
FT CHAIN 1..5154
FT /note="Hydrocephalus-inducing protein"
FT /id="PRO_0000284845"
FT REGION 409..800
FT /note="Interaction with KIF9"
FT /evidence="ECO:0000269|PubMed:32072696"
FT REGION 997..1033
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1966..1988
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2193..2222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2383..2423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2521..2572
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2706..2762
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1948..1977
FT /evidence="ECO:0000255"
FT COILED 2308..2444
FT /evidence="ECO:0000255"
FT COILED 2543..2588
FT /evidence="ECO:0000255"
FT COMPBIAS 997..1012
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1013..1031
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1974..1988
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2204..2222
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2383..2412
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2542..2572
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2716..2742
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..38
FT /note="MTLKIKCVANYIKEKIPNVLFLCDPEARLQQLTASVPL -> MDSAVQQKVA
FT RKKCRD (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_040915"
FT VAR_SEQ 489..501
FT /note="GREIRLPLRIRGE -> VRSTEDGILGPLQ (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_040916"
FT VAR_SEQ 502..5154
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_040917"
FT VAR_SEQ 1333
FT /note="A -> V (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024701"
FT VAR_SEQ 1334..5154
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024702"
FT CONFLICT 97
FT /note="F -> S (in Ref. 2; BB641870)"
FT /evidence="ECO:0000305"
FT CONFLICT 107
FT /note="Q -> H (in Ref. 2; BB641870)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="I -> M (in Ref. 2; BAE25238)"
FT /evidence="ECO:0000305"
FT CONFLICT 1264
FT /note="A -> T (in Ref. 2; BAE25238)"
FT /evidence="ECO:0000305"
FT CONFLICT 4370
FT /note="L -> F (in Ref. 3; AAH80316)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 5154 AA; 581523 MW; 64BD17692EAFD2BA CRC64;
MTLKIKCVAN YIKEKIPNVL FLCDPEARLQ QLTASVPLCE QRKYFKQTNK RLEESMGAMH
RKMAKVISGL QSKVLPPMSP KVVTEEEVNR MLTPSEFLKE MSLTTEQKLA STRIICRPQI
TELLDMGETT HQKFSRVDLD QALFQPFPSE IIFQNYSPCE VYEVPLVLRN NDKIPRMVKV
VEESSPYFKI ISPKDIGHKV APGVPSVFRI LFTPEENKDY AHMLTCITER EKFIVPVKAR
GARAILDFPD ELNFSTCPVK YNTQKVLLVR NIGNKDSMFH LKTRSPYSVE PTGGILNVGE
SMQLEVDFEP QTVGSHDGKL IVTYDTGEMV FVCLYGVAID VNIRLDKNSL IMEKTYISLA
NQRSITIHNR TNIIAHFQWK VFATEEEEDK EKYKICDGLN KEEKQETSMI LEDSVLDPSL
RERLSIISRT FENQRKLVQG DSMLFLDHVF TIEPPEGDVW PNSSAEITVY FNPLEAKLYQ
QTVYCDISGR EIRLPLRIRG EGMGPKLHFN FELLDIGKVF IGSAHCYEAI LSNKGSIDAL
FNVIPPTSAL GACFVFNPKE GIIEPSGVQA VQISFSSTIL GYFEEEFLID VNGSPEPVKM
TIRGCVIGPT FHFNVPALNF GNVSYGFPHT LMCSLNNTSL VPMTFKLRVR GDGEGMSSIP
SYSQESDSKQ WSGINTEMPT TKPKEFTISP NSGTIRAQGF TAIKVTLCSN TVQKYELALV
VDVEGIGEEV LALLITARCV VPKLQLVTTE VDFGRCFLKY PYEKTIQLVN HDDLPGCYKV
LPQLYENSPP VLLSSPSPCG VISPHSTVSI PLALETQVIG AHRSIVYISV FGSQEAPLAC
NIQSIGEGPV IFIHPTQIDF GNIYVLKDTS RILQLSNQSF IPAVFRVRMA NKKSLWTVKP
SEGAVPAEDD IPLTLTANLD DIVTFKDTVI LEIKHSNTYR IPIQATGIGS TIVSDKPFAP
ELNLGAHFSL DTHYYRFKLT NKGRRVQQLF WMNDDFRPKE KQSKKEPGKK GSTSSSRRQS
KASQEPTDNG NPVFQLYPVR MELYPGQTID VILEGYSATP KKVKEKLVCQ AIVGTQKGKS
LLMSVNIICE FIAPIIQLSA KQLLYRLEKK PNSNLESDYQ SLVIKNITTL PVNMLLSTTR
PFFICETDKS LLPATPKPIK LEVDEEKHLL IKFDPSYRND LNNWVAEEVL SIKYLEHPQV
DNLGLRAEVH YPNLSFEVMD IDFGCILNDT EVIRYIMITN CSPLVVKFRW FFLVDEEENQ
IRFAPCMKPY SAFLSQMESI AATSVTASSL AAPHTVEYTE MDFSDSIKTI LMDDEAGPEE
IKKPATSTMV SEAIKYSSAG TERSQSQPDY PECMWIYEQD EMLSIGIEEV FDILPLYGVL
RPYSSHQISF TFYGHCDIIA RAKALCEVEG GPTYEVLLKG EASLVNYSFD TKDINYGLQL
FDHVTEREIT LKNTGKVGFE FNVLSNYRSS QRNLLPGVPL ILPLSGFIQS NKEQVLKVYY
LPGIPEVFQR NFQIQIAHLV PENITLYGEG IFPRISLDLH RNLQGNEKYE HFLEQAKKNV
EKEYTKYEAV DQYEVMAEEL PEEETAEISA HVQMEVERLI VQDYALEHQR SISNNTEDIY
FSQRSCRKLT KVQLPEYILD FGYIVLGDVR THIVKITNTS HFPVSFHAEK QVLHETGFST
ELDRVKNLPY YETETFEVRC DPQGANLPVG NKEVILPIKV YGGPTIHLCL QATVIIPSMT
LSCNKIEFAT IQCGQCMVET IQLSNHLQVP CEWFVHTPKS TNKLDKHMPK YLRRKLQAEM
IPKSRIFEIQ PTSGILDPGE RANVQVKFMP KEEKLYSQSL LFQIGQSSQK LTLLAQGQGL
EPRLEFSPSV LELGPLLPFA SGDEAEVIVR NPCNFPIEFY SLEFDQQYLL EEKMLRQLKG
YDSYNTLLLP PRNPGEKLPP EVYDYFKEMK KSKEEHMKAK YMENLENEEE EMNTSDQGTT
STKRTSISRG ISVTSNLEER HLTIEAKNYL DEDDYEESLE KLTFQTDKMQ STDSHSVEEV
GEVESNPVSK AIARHLGIDI SAEGRLAKNR KGIAIIVHGT PLSGKTANAI SMAKFYNAAC
LNIDSIVLEA LSDTNNILGI RARELCIRAA IEQSMREAEE SAHESSMTQN TVVPARLSTE
NLGRFTSELT LITQEYKVPK TVRGSVMLPK GKADSHFTGS QKQHHQHQSE TPQVQISSSP
LLPGPTHRRL SVSASIGGET GLMSCVLPDD LLIQILAERI QLSNCFRGVV FDGLDTLFAR
NAPSALHCLL KAIGSREHIY VINMSQDYVV MKAQEKAKKE QEENKRKEAL AKEKERLQTL
DEDEYDALTA EEKVAFDRDV RQALRERKKR ELERLAKEMQ EKKLQQELER QKEEDELKRK
VKRPKAGPAA KEEPPLKKAQ GATNKQLAAV AKIELKMESI ERKVSVREHA TLEETTRKKK
AMTEYPLLIP ISQEQEDSEG DFLKDSDKNL AQKFKIYDMC LKDVQNILMY WDRKQGMMVP
HTGTDEMSHE ADDQRQAPSG GGGRKGRKDR ERERLEKERA EKERLEREKA ERERLEKLKA
LEERSDVEGE GEEEHEGKKD LGVPFINIQS PDFEGVSWKQ ALESDKLPKG DQILDILGLG
SSGPPIPPPV LFSIISYPAK RQSLVATEIL KHFVFVIPPN DDIPLMDEKK DPEGDSDIFL
NTIITKAQEE QPSPPKGSKQ KLKDKPEQVR ETQKEKRRTY SSRKGLPGGT SGSIVPMSDI
DQNSFDGEHS QEKFIRLNHF RWIVPPNGEV TLRVHFSSLD VGNYDQTFNF ELLGTRRQYQ
LYCRGVCTYP YICRDPKVVF SQRKKDMKLK EVVVKKYVVS MEKFYFGPLL CGKSRDKYKS
SLFPGNMETL TILNDSPMVV EAYFCFQHDI KASTYFLEPV NMTLKPNEKQ ALNVWAYPTA
VGIFEDSIVC CIKENPEPAI FKLSCQGIRP EIEVEPRQLH FDRLLLHRKE TKIVILRNVT
PLPVAWRISN LEHLGEDFTV SMMQGTMLPK GEYGLQVHFQ PSKPVNIKKA IRIEVLDAEN
LVGVVQIENI LIFAESYDIA LDITFPKGAE GGLDFGTLRV MEEVKQTLQL KNRGKYEIIF
SFTVDTLGVL PTNLSSMISV QPKRGTLASI DKPTTVQVFF RARKEVKIDC QPILRCQIIE
PTLPEGEIIA SIPIKFSVNA VYSKFTISPS SIINFGALIC GTRKSITFTI ENQGIIDFKY
ALYRLTGESP ILQKKITSHM RHGRTRESES FYKPGATKMA KFSDTVQKDT NIANQARFTH
GMFTVYPGFG SIPSGGQQVI TIECFADPVG RCEEFLAIDI SDRDPRENPA GIPYTLLAEA
CLPAFVTDNN ILIFEEHQIC TSPNLYHILQ TIQSGGLFVE DENKFIFCNV LVGHQAKARF
KISNVGKISC DINIVIKPIS NKPANRITDT FDVEPSKMCI GSRSHAFVTV LFTPQTMQTY
QCIFEATLDG LPSNIARSRG LVFDIVGEGT LPRVTVIRPT LYNQHGNPLL LFKRLLLGHS
EKLPLILKNN GTIPAQLHVD LRDQLGVFSL KGRPTTSYIY IMEENKPNEK VKKAHTASLV
VSPGDTAEFD VFFHSNKIGR MTGTIHLSVI NNQYEETMIH LVGESYEDDI TLDNIHGLIS
STSQESSDKS EVIEIAEEST MEDLVTAALM EHIQFGYCHI GISYNVSFTI TNHSQVNVIR
FEWPLLATLS FSPQIGHLHP GCSKDVVVTL KSESPITLKK MCVKCKLSKI MFQLPVDQVP
DWDDRMRTVK WVDAPRNTPL TLNTKRKVIE MDPEPAHSVV EENYRELRIQ FSANVDFASY
QCETTEVFFK ETLVYQTRVF EFDLVNTGQV LLEFCWISEE ASKAVSFAMP ERQGSSQKEL
SQGSGSSLDS ALDRWTEASP SPFSVEPPSG VVPVGKTQKL KVKFSPMDIG EFESSLYCQI
PNLPLGEQGP ILITKGRSVL PFCHFDLKES DYISGHRRNP ELRGPGAGPL EPNTRVIEFT
SVGIGGKNVQ TFTILNPTNS TYSFCWTSEE TESLQHPPAF VCLTEKGIIH PEKKAEIIFQ
FLPAHLDITE EFWTFSIPEH NISVPFLLVG KTTDPLISLD KSHLNFSSLL IGREARETVK
IINKEEQGFN FAFQDNSRYS EGFNNSLIVC PMEGWIPPLS RFPVDIFFTP KQEGDVNFNL
ICNIKKKAHP LTLNVKAEGY TMNAEVKCRD RMGTTILLTS TQVNTINFYE VELNECVQCE
FSFINTGKFN FSYQAELSGP KLLLQYLDFT PTDSSVDVGQ SEPANLSFQP YQKCVLKGLE
LKIKISHGPT FVCNILGCAV SPAVHFSFTS HNFGTCFIYQ AGMPPYKQIL VVTNKEETSM
SLDCLYTNTP HIEVNFNVDV IKPGKTLEIP ITFYPREAIS YRELIPFEIN GLSQQTVEIK
GKGTEMKLLV LDPANRIVKL GAVLPGQVVR KTVSLVNNSL AQLTFNHSVL FSIPELQEAK
VITLEPFHTI TMKPKEVCKL EITFAPKKRV PPFSEEVFME WMGLLRPLFL LSGCCQALEI
SLDQEHLPFG PVVYQTQATR RILIMNTGDV GARFKWDVKK LKPHFSISPE EGYIISGTEV
ALEVTYHPTE IGKESLYKNI LCFIQGGNPL CLTLSGTCVG PPVVKEVVNF NCQVRSRHTQ
TILLSNRSNQ TWNLHPIFEG EHWEGPEFIT LEAHQQNKPY EITYKPRTMN LENRKHQGTL
FFPLPDGTGW LYALHGTAEL PKAVANIYRE VPCKTPYTEL LPISNWLNKP QRFRVIVEIL
KPEKTDLSVT LKGLDYIDVL SGSKKDYKLN FFSYKEGLYT AKVIFRNEVT NEFLYYTVSF
RVTPSGIIKT IQMTSPVRQS VSASIKLENP LPYSVTFSTE CKLSDISLPS QFAVPPNSEG
TFSFEFQPLK AGELCGRLTL HNSDLGYYQY ELALKALPAP PEKPVHFQTV LGSSQSILAK
FTNYTRLKTE YYCKTDCSDF HTEKVINAAP GAQGGTEVSV EVFFEPSHLG ETKGILCLSS
LIGGEYIIPL FGIALPPKPQ GPFLIRAGYN IIIPFKNVFL HATSFSFIVE NPAFSIRAAE
TVRPKKINNI TVYFEGNPSG SKTPITSKLI VTCPQCEGTE SGIKWVYYLK GITP
