ID   HYDL_STRCO              Reviewed;         627 AA.
AC   P42534; Q9S2L7;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Putative polyketide hydroxylase;
DE            EC=1.14.13.-;
DE   AltName: Full=WhiE ORF VIII;
GN   OrderedLocusNames=SCO5321; ORFNames=SC6G9.12c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-255.
RC   STRAIN=A3(2) / NRRL B-16638;
RX   PubMed=8253693; DOI=10.1128/jb.175.24.8043-8048.1993;
RA   Blanco G., Pereda A., Brian P., Mendez C., Chater K.F., Salas J.A.;
RT   "A hydroxylase-like gene product contributes to synthesis of a polyketide
RT   spore pigment in Streptomyces halstedii.";
RL   J. Bacteriol. 175:8043-8048(1993).
CC   -!- FUNCTION: Involved in developmentally regulated synthesis of a compound
CC       biosynthetically related to polyketide antibiotics which is essential
CC       for spore color in Streptococcus coelicolor.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the PheA/TfdB FAD monooxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; AL939123; CAB45603.1; -; Genomic_DNA.
DR   EMBL; X74213; CAA52289.1; -; Genomic_DNA.
DR   PIR; T35608; T35608.
DR   RefSeq; NP_629463.1; NC_003888.3.
DR   RefSeq; WP_011030172.1; NZ_VNID01000040.1.
DR   AlphaFoldDB; P42534; -.
DR   SMR; P42534; -.
DR   STRING; 100226.SCO5321; -.
DR   PRIDE; P42534; -.
DR   GeneID; 1100761; -.
DR   KEGG; sco:SCO5321; -.
DR   PATRIC; fig|100226.15.peg.5407; -.
DR   eggNOG; COG0654; Bacteria.
DR   HOGENOM; CLU_009665_14_2_11; -.
DR   InParanoid; P42534; -.
DR   OMA; GWCLCSQ; -.
DR   PhylomeDB; P42534; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   FAD; Flavoprotein; Oxidoreductase; Reference proteome.
FT   CHAIN           1..627
FT                   /note="Putative polyketide hydroxylase"
FT                   /id="PRO_0000214041"
FT   REGION          370..469
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         22..51
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   BINDING         309..319
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        60
FT                   /note="R -> A (in Ref. 2; CAA52289)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        145
FT                   /note="L -> LH (in Ref. 2; CAA52289)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        234
FT                   /note="C -> S (in Ref. 2; CAA52289)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   627 AA;  64558 MW;  746E84A2A9E9511C CRC64;
     MNERADRSGG AAPGGDRTHR VPVLVVGGSL VGLSTSVFLG RLGVRHTLVE RHAGTSIHPR
     GRGNNVRTME IFRVAGTEPD IRRAAATLAD NHGILQAPTL AGDAGEWLFK QIDPGGGLAR
     FSPSSWCLCS QNDLEPELLT HATNLGGDLR FGTELLSFEA DTEGVTAIVK SRETGEHTTI
     RADYLVAADG PRSPVREQLG IGQSGPGDLF HNVSITFRSR RLADVVGDRR FIVCYLTDEN
     ADGALLPVDN RENWVFHAPW HPEQGETVED FTDERCAAHI RRAIGDPDLD VEITGKAPWH
     AAQRVARSYR SGRVLLAGDS AHEMSPTGAF GSNTGIQDAH NLAWKLAAVL EGWAGEALLD
     TYDTERRPVA EATSARAAHR SVEHSHPGFA PPPVAGGGGP GAGTPGGAGR GTGGPGGPGG
     PGGLGGPGGP GGTGGPGGPG GPGGPDGPRG AGGAPGGGPG GGPGGGGPQR GILNVALGYR
     YPRGAVVGAD PATPVVPEGL DLTGAPGSRA PHLWVRRGQD RLSTLDLYED SLVLLSDAAQ
     PTGWHEAAAG VAAGMRVPLK SYRVGGSPGA DLNPDDEETD WARAHGVTRG GAVLVRPDGF
     VAWRSPGPAP DPESMLRQVV GTVLARS