HYFD_ECOLI
ID HYFD_ECOLI Reviewed; 479 AA.
AC P77416; P76973;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Hydrogenase-4 component D;
DE EC=1.-.-.-;
GN Name=hyfD {ECO:0000303|PubMed:9387241}; OrderedLocusNames=b2484, JW2469;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND POSSIBLE FUNCTION.
RC STRAIN=K12;
RX PubMed=9387241; DOI=10.1099/00221287-143-11-3633;
RA Andrews S.C., Berks B.C., McClay J., Ambler A., Quail M.A., Golby P.,
RA Guest J.R.;
RT "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-
RT translocating formate hydrogenlyase system.";
RL Microbiology 143:3633-3647(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Possible component of hydrogenase 4.
CC {ECO:0000305|PubMed:9387241}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; M63654; AAB88566.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75537.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16372.1; -; Genomic_DNA.
DR PIR; C65024; C65024.
DR RefSeq; NP_416979.1; NC_000913.3.
DR RefSeq; WP_000429104.1; NZ_LN832404.1.
DR AlphaFoldDB; P77416; -.
DR SMR; P77416; -.
DR BioGRID; 4259198; 19.
DR ComplexPortal; CPX-6028; Formate hydrogenlyase-H/Hydrogenase-4 complex.
DR STRING; 511145.b2484; -.
DR TCDB; 3.D.1.9.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P77416; -.
DR PRIDE; P77416; -.
DR DNASU; 947290; -.
DR EnsemblBacteria; AAC75537; AAC75537; b2484.
DR EnsemblBacteria; BAA16372; BAA16372; BAA16372.
DR GeneID; 947290; -.
DR KEGG; ecj:JW2469; -.
DR KEGG; eco:b2484; -.
DR PATRIC; fig|1411691.4.peg.4255; -.
DR EchoBASE; EB3964; -.
DR eggNOG; COG1009; Bacteria.
DR HOGENOM; CLU_007100_0_1_6; -.
DR InParanoid; P77416; -.
DR OMA; YVAHHIT; -.
DR PhylomeDB; P77416; -.
DR BioCyc; EcoCyc:MON0-141; -.
DR PRO; PR:P77416; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0003954; F:NADH dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0019645; P:anaerobic electron transport chain; IC:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IC:ComplexPortal.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0015944; P:formate oxidation; IC:ComplexPortal.
DR GO; GO:0006007; P:glucose catabolic process; IC:ComplexPortal.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR003945; NU5C-like.
DR InterPro; IPR001516; Proton_antipo_N.
DR PANTHER; PTHR42829; PTHR42829; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..479
FT /note="Hydrogenase-4 component D"
FT /id="PRO_0000118227"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 369..389
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 479 AA; 51755 MW; 61777107CC9100F5 CRC64;
MENLALTTLL LPFIGALVVS FSPQRRAAEW GVLFAALTTL CMLSLISAFY QADKVAVTLT
LVNVGDVALF GLVIDRVSTL ILFVVVFLGL LVTIYSTGYL TDKNREHPHN GTNRYYAFLL
VFIGAMAGLV LSSTLLGQLL FFEITGGCSW ALISYYQSDK AQRSALKALL ITHIGSLGLY
LAAATLFLQT GTFALSAMSE LHGDARYLVY GGILFAAWGK SAQLPMQAWL PDAMEAPTPI
SAYLHAASMV KVGVYIFARA IIDGGNIPHV IGGVGMVMAL VTILYGFLMY LPQQDMKRLL
AWSTITQLGW MFFGLSLSIF GSRLALEGSI AYIVNHAFAK SLFFLVAGAL SYSCGTRLLP
RLRGVLHTLP LPGVGFCVAA LAITGVPPFN GFFSKFPLFA AGFALSVEYW ILLPAMILLM
IESVASFAWF IRWFGRVVPG KPSEAVADAA PLPGSMRLVL IVLIVMSLIS SVIAATWLQ
