HYFE_ECOLI
ID HYFE_ECOLI Reviewed; 216 AA.
AC P0AEW1; P77524;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Hydrogenase-4 component E;
DE EC=1.-.-.-;
GN Name=hyfE {ECO:0000303|PubMed:9387241}; OrderedLocusNames=b2485, JW2470;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND POSSIBLE FUNCTION.
RC STRAIN=K12;
RX PubMed=9387241; DOI=10.1099/00221287-143-11-3633;
RA Andrews S.C., Berks B.C., McClay J., Ambler A., Quail M.A., Golby P.,
RA Guest J.R.;
RT "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-
RT translocating formate hydrogenlyase system.";
RL Microbiology 143:3633-3647(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Possible component of hydrogenase 4.
CC {ECO:0000305|PubMed:9387241}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:9278503};
CC Multi-pass membrane protein.
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DR EMBL; M63654; AAB88567.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75538.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16373.1; -; Genomic_DNA.
DR PIR; D65024; D65024.
DR RefSeq; NP_416980.1; NC_000913.3.
DR RefSeq; WP_000147987.1; NZ_LN832404.1.
DR AlphaFoldDB; P0AEW1; -.
DR SMR; P0AEW1; -.
DR BioGRID; 4263214; 171.
DR ComplexPortal; CPX-6028; Formate hydrogenlyase-H/Hydrogenase-4 complex.
DR DIP; DIP-48020N; -.
DR IntAct; P0AEW1; 2.
DR STRING; 511145.b2485; -.
DR TCDB; 3.D.1.9.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P0AEW1; -.
DR PRIDE; P0AEW1; -.
DR EnsemblBacteria; AAC75538; AAC75538; b2485.
DR EnsemblBacteria; BAA16373; BAA16373; BAA16373.
DR GeneID; 66673650; -.
DR GeneID; 945298; -.
DR KEGG; ecj:JW2470; -.
DR KEGG; eco:b2485; -.
DR PATRIC; fig|1411691.4.peg.4254; -.
DR EchoBASE; EB3965; -.
DR eggNOG; COG4237; Bacteria.
DR HOGENOM; CLU_088957_2_0_6; -.
DR InParanoid; P0AEW1; -.
DR OMA; VFGYCLM; -.
DR PhylomeDB; P0AEW1; -.
DR BioCyc; EcoCyc:MON0-142; -.
DR PRO; PR:P0AEW1; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0019645; P:anaerobic electron transport chain; IC:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IC:ComplexPortal.
DR GO; GO:0015944; P:formate oxidation; IC:ComplexPortal.
DR GO; GO:0006007; P:glucose catabolic process; IC:ComplexPortal.
DR InterPro; IPR038730; HyfE-like.
DR InterPro; IPR039428; NUOK/Mnh_C1-like.
DR PANTHER; PTHR38601; PTHR38601; 1.
DR Pfam; PF00420; Oxidored_q2; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..216
FT /note="Hydrogenase-4 component E"
FT /id="PRO_0000084115"
FT TOPO_DOM 1..3
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..92
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..122
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..150
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..175
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..216
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255, ECO:0000269|PubMed:9278503"
SQ SEQUENCE 216 AA; 23361 MW; 141A76B9961D8F4F CRC64;
MTGSMIVNNL AGLMMLTSLF VISVKSYRLS CGFYACQSLV LVSIFATLSC LFAAEQLLIW
SASAFITKVL LVPLIMTYAA RNIPQNIPEK ALFGPAMMAL LAALIVLLCA FVVQPVKLPM
ATGLKPALAV ALGHFLLGLL CIVSQRNILR QIFGYCLMEN GSHLVLALLA WRAPELVEIG
IATDAIFAVI VMVLLARKIW RTHGTLDVNN LTALKG
