HYFF_ECOLI
ID HYFF_ECOLI Reviewed; 526 AA.
AC P77437;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Hydrogenase-4 component F;
DE EC=1.-.-.-;
GN Name=hyfF {ECO:0000303|PubMed:9387241}; OrderedLocusNames=b2486, JW2471;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND POSSIBLE FUNCTION.
RC STRAIN=K12;
RX PubMed=9387241; DOI=10.1099/00221287-143-11-3633;
RA Andrews S.C., Berks B.C., McClay J., Ambler A., Quail M.A., Golby P.,
RA Guest J.R.;
RT "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-
RT translocating formate hydrogenlyase system.";
RL Microbiology 143:3633-3647(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Possible component of hydrogenase 4.
CC {ECO:0000305|PubMed:9387241}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the complex I subunit 5 family. {ECO:0000305}.
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DR EMBL; M63654; AAB88568.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75539.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16374.1; -; Genomic_DNA.
DR PIR; E65024; E65024.
DR RefSeq; NP_416981.1; NC_000913.3.
DR RefSeq; WP_000122577.1; NZ_LN832404.1.
DR AlphaFoldDB; P77437; -.
DR SMR; P77437; -.
DR BioGRID; 4262930; 13.
DR ComplexPortal; CPX-6028; Formate hydrogenlyase-H/Hydrogenase-4 complex.
DR IntAct; P77437; 1.
DR STRING; 511145.b2486; -.
DR TCDB; 3.D.1.9.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P77437; -.
DR PRIDE; P77437; -.
DR DNASU; 946962; -.
DR EnsemblBacteria; AAC75539; AAC75539; b2486.
DR EnsemblBacteria; BAA16374; BAA16374; BAA16374.
DR GeneID; 946962; -.
DR KEGG; ecj:JW2471; -.
DR KEGG; eco:b2486; -.
DR PATRIC; fig|1411691.4.peg.4253; -.
DR EchoBASE; EB3966; -.
DR eggNOG; COG0651; Bacteria.
DR HOGENOM; CLU_007100_10_1_6; -.
DR InParanoid; P77437; -.
DR OMA; VEAAWKY; -.
DR PhylomeDB; P77437; -.
DR BioCyc; EcoCyc:MON0-143; -.
DR PRO; PR:P77437; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0009326; C:formate dehydrogenase complex; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0019645; P:anaerobic electron transport chain; IC:ComplexPortal.
DR GO; GO:0009061; P:anaerobic respiration; IC:ComplexPortal.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0015944; P:formate oxidation; IC:ComplexPortal.
DR GO; GO:0006007; P:glucose catabolic process; IC:ComplexPortal.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR001516; Proton_antipo_N.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Oxidoreductase;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..526
FT /note="Hydrogenase-4 component F"
FT /id="PRO_0000118228"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 322..342
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 526 AA; 56767 MW; 229068F616880775 CRC64;
MSYSVMFALL LLTPLLFSLL CFACRKRRLS ATRTVTVLHS LGITLLLILA LWVVQTAADA
GEIFAAGLWL HIDGLGGLFL AILGVIGFLT GIYSIGYMRH EVAHGELSPV TLCDYYGFFH
LFLFTMLLVV TSNNLIVMWA AIEATTLSSA FLVGIYGQRS SLEAAWKYII ICTVGVAFGL
FGTVLVYANA ASVMPQAEMA IFWSEVLKQS SLLDPTLMLL AFVFLLIGFG TKTGLFPMHA
WLPDAHSEAP SPVSALLSAV LLNCALLVLI RYYIIICQAI GSDFPNRLLL IFGMLSVAVA
AFFILVQRDI KRLLAYSSVE NMGLVAVELG IGGPLGIFAA LLHILNHSLA KTLLFCGSGN
VLLKYGTRDL NVVCGMLKIM PFTAVLFGGG ALALAGMPPF NIFLSEFMTI TAGLARNHLL
IIVLLLLLLT LVLAGLVRMA ARVLMAKPPQ AVNRGDLGWL TTSPMVILLV MMLAMGTHIP
QPVIRILAGA STIVLSGTHD LPAQRSTWHD FLPSGTASVS EKHSER
