ID   HYFLC_HYBFL             Reviewed;          32 AA.
AC   P84649;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Cyclotide Hyfl-C;
OS   Hybanthus floribundus (Greenviolet).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Violaceae; Hybanthus.
OX   NCBI_TaxID=343459;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RX   PubMed=16199617; DOI=10.1105/tpc.105.034678;
RA   Simonsen S.M., Sando L., Ireland D.C., Colgrave M.L., Bharathi R.,
RA   Goeransson U., Craik D.J.;
RT   "A continent of plant defense peptide diversity: cyclotides in Australian
RT   Hybanthus (Violaceae).";
RL   Plant Cell 17:3176-3189(2005).
CC   -!- FUNCTION: Probably participates in a plant defense mechanism.
CC       {ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000250|UniProtKB:P56879}.
CC   -!- PTM: This is a cyclic peptide. {ECO:0000255|PROSITE-ProRule:PRU00395,
CC       ECO:0000269|PubMed:16199617}.
CC   -!- SIMILARITY: Belongs to the cyclotide family. Moebius subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00395}.
CC   -!- CAUTION: This peptide is cyclic. The start position was chosen by
CC       similarity to OAK1 (kalata-B1) for which the DNA sequence is known.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P84649; -.
DR   SMR; P84649; -.
DR   GO; GO:0051715; P:cytolysis in another organism; IEA:UniProt.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   InterPro; IPR005535; Cyclotide.
DR   InterPro; IPR012324; Cyclotide_moebius_CS.
DR   InterPro; IPR036146; Cyclotide_sf.
DR   Pfam; PF03784; Cyclotide; 1.
DR   PIRSF; PIRSF037891; Cycloviolacin; 1.
DR   SUPFAM; SSF57038; SSF57038; 1.
DR   PROSITE; PS51052; CYCLOTIDE; 1.
DR   PROSITE; PS60009; CYCLOTIDE_MOEBIUS; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Knottin; Plant defense.
FT   PEPTIDE         1..32
FT                   /note="Cyclotide Hyfl-C"
FT                   /id="PRO_0000044707"
FT   DISULFID        6..22
FT                   /evidence="ECO:0000250|UniProtKB:P56879,
FT                   ECO:0000255|PROSITE-ProRule:PRU00395"
FT   DISULFID        10..24
FT                   /evidence="ECO:0000250|UniProtKB:P56879,
FT                   ECO:0000255|PROSITE-ProRule:PRU00395"
FT   DISULFID        15..29
FT                   /evidence="ECO:0000250|UniProtKB:P56879,
FT                   ECO:0000255|PROSITE-ProRule:PRU00395"
FT   CROSSLNK        1..32
FT                   /note="Cyclopeptide (Gly-Asn)"
FT                   /evidence="ECO:0000269|PubMed:16199617"
SQ   SEQUENCE   32 AA;  3509 MW;  786F0DFEF6CF2B4B CRC64;
     GSPRQCAETC FIGKCYTEEL GCTCTAFLCM KN