ID   HYIN1_AGRVS             Reviewed;         462 AA.
AC   Q04557; B9K453;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Indoleacetamide hydrolase;
DE            Short=IAH;
DE            EC=3.5.1.-;
DE   AltName: Full=Indole-3-acetamide hydrolase;
GN   Name=iaaH; Synonyms=tms2; OrderedLocusNames=Avi_8287;
OS   Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain
OS   S4)).
OG   Plasmid pTiS4.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=311402;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1465104; DOI=10.1007/bf00279373;
RA   Canaday J., Gerard J.-C., Crouzet P., Otten L.;
RT   "Organization and functional analysis of three T-DNAs from the vitopine Ti
RT   plasmid pTiS4.";
RL   Mol. Gen. Genet. 235:292-303(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S4 / ATCC BAA-846;
RX   PubMed=19251847; DOI=10.1128/jb.01779-08;
RA   Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K.,
RA   Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L.,
RA   Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A.,
RA   Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M.,
RA   Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N.,
RA   Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W.;
RT   "Genome sequences of three Agrobacterium biovars help elucidate the
RT   evolution of multichromosome genomes in bacteria.";
RL   J. Bacteriol. 191:2501-2511(2009).
CC   -!- FUNCTION: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid
CC       (IAA).
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; M91609; AAA98148.1; -; Genomic_DNA.
DR   EMBL; CP000637; ACM39707.1; -; Genomic_DNA.
DR   PIR; S30104; S30104.
DR   RefSeq; WP_012649066.1; NC_011982.1.
DR   AlphaFoldDB; Q04557; -.
DR   SMR; Q04557; -.
DR   PRIDE; Q04557; -.
DR   EnsemblBacteria; ACM39707; ACM39707; Avi_8287.
DR   KEGG; avi:Avi_8287; -.
DR   HOGENOM; CLU_009600_0_3_5; -.
DR   OMA; CGGDTMN; -.
DR   OrthoDB; 1239251at2; -.
DR   UniPathway; UPA00151; -.
DR   Proteomes; UP000001596; Plasmid pTiS4.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Crown gall tumor; Hydrolase; Plasmid;
KW   Reference proteome.
FT   CHAIN           1..462
FT                   /note="Indoleacetamide hydrolase"
FT                   /id="PRO_0000105244"
FT   ACT_SITE        74
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        149
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        173
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   462 AA;  49810 MW;  A4B78DAB34BBFAE5 CRC64;
     MVEISSISQT LRALRRKQYS CRDLVESLVS RSESATHLNA FAATDWLHLR NEADRVDRNG
     SGGVGLMGIP LCFKANIATG IFPTSAGTQG LLRHKPAIPA KIVERLHSAG ALIGASGNMH
     ELSFGITNDN KTFGPARNPW NQALISGGSS GGVAVSVAAN LMLGGIGTDT GASVRLPAAL
     CGVVGFRPTF GNYPTDGIVP VSPSRDTPGL IVRSVEDAVL LDRIIRNKCP TQNMSLKGLR
     LGLPRSHFFD NLEPHVAAAS ERAIRRLATN QMTFVEADIP NVAELTRKVS LPVAIYEFPR
     ALMAYLSFHG IGNTFDELIQ NIQDPQVYDL VQSQLSKGLI SESVYRRALR CYKPRLKATY
     ENYYSSNGLD AVLFPSVPLT AKPVGLQTTL VHNGVETDTF GIFVRNLDPS SNIGLPSLSV
     PVSLTPDRLP VGIQIEGPFG SDDMVLAIGR AVEEMVKFGQ EY