ID   HYIN2_AGRVI             Reviewed;         467 AA.
AC   P25016;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Indoleacetamide hydrolase;
DE            Short=IAH;
DE            EC=3.5.1.-;
DE   AltName: Full=Indole-3-acetamide hydrolase;
GN   Name=TA-iaaH;
OS   Agrobacterium vitis (Rhizobium vitis).
OG   Plasmid pTiTM4.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=373;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TM4;
RX   PubMed=1868204; DOI=10.1007/bf00023438;
RA   Bonnard G., Vincent F., Otten L.;
RT   "Sequence of Agrobacterium tumefaciens biotype III auxin genes.";
RL   Plant Mol. Biol. 16:733-738(1991).
CC   -!- FUNCTION: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid
CC       (IAA).
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
CC   -!- CAUTION: The plasmid pTiTM4 carries two T-regions, the TA and TB
CC       region, both of which have an IaaH gene, with low homology between
CC       them. Only the TB-IaaH gene seems to be functional. {ECO:0000305}.
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DR   EMBL; X56185; CAA39649.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; P25016; -.
DR   SMR; P25016; -.
DR   UniPathway; UPA00151; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Crown gall tumor; Hydrolase; Plasmid.
FT   CHAIN           1..467
FT                   /note="Indoleacetamide hydrolase"
FT                   /id="PRO_0000105245"
FT   ACT_SITE        74
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        149
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        173
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   467 AA;  49942 MW;  8ABC04ADB58A1700 CRC64;
     MVPITSLAQT LERLRRKDYS CLELVETLIA RCEAAKALNA LLATDWGGLR RRAKKIDRHG
     NAGVGLRGIP LCFKANIATG IFPTTAATPA LINHLPKIPS RIAERLFSAG ALPGASGNMH
     ELSFGITSNN YATGAVRNPW NPSLIPGGSS GGVAAAVASR LMLGGIGTDT GASVRLPAAL
     CGVVGFRPTL GRYPRDRIIP VSPTRDTAGI IAQCVADVVI LDQVISGRPA RILPIPLKGL
     RIGLPTTYFY DDLDADVAFA AETTIRLLAN RGVTFVEADI PHLEDLNSGA SLPIALYEFP
     HALKQYLDDF VGTVSFSDVI KEIRSPDVAN IVNAQIDGHQ ISNAEYELAR QSFRPRLQAA
     YRNYFRLYRL DAILFPTAPL AAKAIGQDSS VIHNGSMVNT FKIYVRNVDP SSNAGLPGLS
     LPVGLTPDRL PVGMEIDGLA GSDHRLLAIG AALEKAINFR SFPDVLN