ID   HYIN_AGRRH              Reviewed;         466 AA.
AC   Q09102;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Indoleacetamide hydrolase;
DE            Short=IAH;
DE            EC=3.5.1.-;
DE   AltName: Full=Indole-3-acetamide hydrolase;
GN   Name=aux2;
OS   Agrobacterium rhizogenes.
OG   Plasmid pRiA4.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A4;
RX   PubMed=1932811; DOI=10.1094/mpmi-4-155;
RA   Camilleri C., Jouanin L.;
RT   "The TR-DNA region carrying the auxin synthesis genes of the Agrobacterium
RT   rhizogenes agropine-type plasmid pRiA4: nucleotide sequence analysis and
RT   introduction into tobacco plants.";
RL   Mol. Plant Microbe Interact. 4:155-162(1991).
CC   -!- FUNCTION: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid
CC       (IAA).
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; M61151; AAA22079.1; -; Genomic_DNA.
DR   PIR; I39707; I39707.
DR   AlphaFoldDB; Q09102; -.
DR   SMR; Q09102; -.
DR   STRING; 359.CN09_18945; -.
DR   eggNOG; COG0154; Bacteria.
DR   UniPathway; UPA00151; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Hydrolase; Plasmid.
FT   CHAIN           1..466
FT                   /note="Indoleacetamide hydrolase"
FT                   /id="PRO_0000105240"
FT   ACT_SITE        74
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        149
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        173
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   466 AA;  49534 MW;  7375A5994B903995 CRC64;
     MVTLSSITET LKCLRERKYS CFELIETIIA RCEAARSLNA FLETDWAHLR WTASKIDQHG
     GAGVGLAGVP LCFKANIATG RFAATAGTPG LQNHKPKTPA GVARQLLAAG ALPGASGNMH
     ELSFGITSNN FATGAVRNPW NPSLIPGGSS GGVAAAVAGR LMLGGVGTDT GASVRLPAAL
     CGVVGFRPTV GRYPTDGIVP VSPTRDTPGV IAQNVPDVIL LDGIICGRPP VNQTVRLKGL
     RIGLPTAYFY NDLEPDVALA AETIIRVLAR KDVTFVEADI PDLAHHNEGV SFPTAIYEFP
     LSLEHYIQNF VEGVSFSEVV RAIRSPDVAS ILNAQLSDNL ISKSEYCLAR RFFRPRLQAA
     YHSYFKAHQL DAILFPTAPL TAKPIGHDLS VIHNGSMTDT FKIFVRNVDP SSNAGLPGLS
     LPVSLSSNGL PIGMEIDGSA SSDERLLAIG LAIEEAIDFR HRPTLS