ID   HYIN_BRADU              Reviewed;         524 AA.
AC   P59385;
DT   28-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   28-FEB-2003, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Indoleacetamide hydrolase;
DE            Short=IAH;
DE            EC=3.5.1.-;
DE   AltName: Full=Indole-3-acetamide hydrolase;
GN   Name=bam; OrderedLocusNames=bll0246;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid
CC       (IAA). {ECO:0000250}.
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; BA000040; BAC45511.1; -; Genomic_DNA.
DR   RefSeq; NP_766886.1; NC_004463.1.
DR   RefSeq; WP_011083078.1; NZ_CP011360.1.
DR   AlphaFoldDB; P59385; -.
DR   SMR; P59385; -.
DR   STRING; 224911.27348493; -.
DR   EnsemblBacteria; BAC45511; BAC45511; BAC45511.
DR   GeneID; 64020107; -.
DR   KEGG; bja:bll0246; -.
DR   PATRIC; fig|224911.44.peg.8766; -.
DR   eggNOG; COG0154; Bacteria.
DR   HOGENOM; CLU_009600_0_4_5; -.
DR   InParanoid; P59385; -.
DR   OMA; TIAHGND; -.
DR   PhylomeDB; P59385; -.
DR   UniPathway; UPA00151; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Hydrolase; Reference proteome.
FT   CHAIN           1..524
FT                   /note="Indoleacetamide hydrolase"
FT                   /id="PRO_0000105246"
FT   REGION          1..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..22
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        29..48
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        133
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        208
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        232
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   524 AA;  56011 MW;  8AC656E2FC382869 CRC64;
     MAKKTASKKK SVSRKVTKTS SKKATARKGA VAKAAKKSVK KAAPRKSATA RRPKGPVWQW
     SAVDTAAAIR SGAISAVETV EAHLDRMRAV NPRLNAVVVD LSKEALKAAH AADKQRAKGG
     ELGLLHGVPI TIKENVDYEG RPNFNGVPAN KDYIAPSDAP VVRNLKKAGA IVIGLTNTPE
     FSFRGFTDNP LHGLTLNPWD PDITCGGSSG GAGSAVAAGI GTIAHGNDIG GSLRWPAHCN
     GVATIKPTQG RIPAFNGSAT AERPMLAHLM SAQGPLARHV ADVRLALEVM SQRDPRDPWW
     VPAPLVGPKP KGPIKVALAK IPDDMDVDPA VAAALRRAAD HLERSGYRVS EVEVPDINGV
     WQTWCDIITN ETMVMQEAAM LKVTSEDFHN AWNGMKAKAN VLDLKAWMQA TAARNGHIRA
     WQLFFEDYPV VLAPTTVSPT PGPRDDTISA ERVQEVFWGG IRFISAINVL GLPGAVVPVA
     LHEGKPIGVQ LITGRYREDL ALDAAAAIEN RAGVLAHRLW ETMD