ID   HYIN_PANAY              Reviewed;         460 AA.
AC   Q47860;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Indoleacetamide hydrolase;
DE            Short=IAH;
DE            EC=3.5.1.-;
DE   AltName: Full=Indole-3-acetamide hydrolase;
GN   Name=iaaH;
OS   Pantoea agglomerans pv. gypsophilae (Erwinia herbicola).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Pantoea; Pantoea agglomerans group.
OX   NCBI_TaxID=48984;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PD713;
RA   Ophir Y., Kunik T., Manulis S.M., Lichter A.L., Barash I.B., Gafni Y.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid
CC       (IAA).
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; L33866; AAC17186.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q47860; -.
DR   SMR; Q47860; -.
DR   UniPathway; UPA00151; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Hydrolase.
FT   CHAIN           1..460
FT                   /note="Indoleacetamide hydrolase"
FT                   /id="PRO_0000105248"
FT   ACT_SITE        71
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        146
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        169
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   460 AA;  49331 MW;  B7C883773B727E94 CRC64;
     MYRKQPLESL CRDLRNGTVS AAEIREQALS AEARLKHLNC FIRDGDAEEQ FSKASDAFKG
     GALWGIPVSF KDNICVKDLP VTAGTPGMTG CIATHDAFIV KKLKSLGAVV AGKNNMHELS
     FGITSVNVQW GTAGNPAAPG YCAGGSSGGC VSRPWSAGLY RSPSDTGGSV RIPAAFCGIA
     GFRPTTGRWA SSGIIPVSHT KDAPGLLTRT VKDAAFLYGL ISGSNTGEPS CEVRKLPCRV
     GLPASLWTGL DDHVERACFT AIRRMQDAGF DCVELDDGGI YALNETLTFT IPLYEFFTDF
     PRALLGLGWE QKIDAVFSHI ADPHVRKIIH AHLAEELISR AEAASAVRDI GRLRLQMNAL
     FQRQNIGLLA YPRHRVTSPC QVPPLSHVTG RSFFAEVIVN TDLASNAALP SVSLPVAPPG
     ALPVGLSFDA PTGQDLFLLK TAAHIEMLLG TSEPDIRTYW