ID   HYIN_PSESY              Reviewed;         446 AA.
AC   P52831;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Indoleacetamide hydrolase;
DE            Short=IAH;
DE            EC=3.5.1.-;
DE   AltName: Full=Indole-3-acetamide hydrolase;
GN   Name=iaaH;
OS   Pseudomonas syringae pv. syringae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Y30;
RX   PubMed=8113177; DOI=10.1128/jb.176.5.1374-1382.1994;
RA   Mazzola M., White F.F.;
RT   "A mutation in the indole-3-acetic acid biosynthesis pathway of Pseudomonas
RT   syringae pv. syringae affects growth in Phaseolus vulgaris and syringomycin
RT   production.";
RL   J. Bacteriol. 176:1374-1382(1994).
CC   -!- FUNCTION: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid
CC       (IAA).
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; U04358; AAA17679.1; -; Unassigned_DNA.
DR   PIR; B53376; B53376.
DR   AlphaFoldDB; P52831; -.
DR   SMR; P52831; -.
DR   UniPathway; UPA00151; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Hydrolase.
FT   CHAIN           1..446
FT                   /note="Indoleacetamide hydrolase"
FT                   /id="PRO_0000105249"
FT   ACT_SITE        71
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        146
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        170
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   446 AA;  47708 MW;  923321538BFD8DAB CRC64;
     MREMITLESL CRALADEQIA AEELRERALD TEARLTLLNC FIREGDAVSQ FGEADQARKG
     TSLWGVPVSF KDNICVRGLP LTAGTRGMSG FIADQDAAIV SQLKALGAVV AGKNNMHELS
     FGVTSINPHW GAVGNPVAPG YCAGGSSGGS AAAVASGIVP LSVGTDTGGS IRIPAAFCGI
     TGFRPTTGRL STAGIIPVSH TKDCVGLLTR TAGDAEFVYG LLSGKQQSFP LNRTGPCRIG
     LPVSMWSDLD GEVERACINA LSLLRKTGFE FVEIDDADIV ELNQTLTFTV PLYEFFADFA
     QSLLSLGWKH GIHHIFAQVD DANVKGIINH HLGEGAIKPA HYLSSLQNGE LLKRKMDELF
     ARHHIKLLGY PTVPCRVPHL DHADRPEFFS QAIRNTDLAS NAMLPSITIP VGPEGRLPVG
     LSFDAPRARD AFLLSNVSLI EKVLKG