ID   HYIN_RHIRD              Reviewed;         467 AA.
AC   P0A2X0; P03868;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Indoleacetamide hydrolase;
DE            Short=IAH;
DE            EC=3.5.1.-;
DE   AltName: Full=Indole-3-acetamide hydrolase;
GN   Name=tms2; Synonyms=iaaH;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6NC.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6366736; DOI=10.1093/nar/12.3.1447;
RA   Sciaky D., Thomashow M.F.;
RT   "The sequence of the tms transcript 2 locus of the A. tumefaciens plasmid
RT   pTiA6 and characterization of the mutation in pTiA66 that is responsible
RT   for auxin attenuation.";
RL   Nucleic Acids Res. 12:1447-1461(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6584906; DOI=10.1073/pnas.81.6.1728;
RA   Klee H., Montoya A., Horodyski F., Lichtenstein C., Garfinkel D.,
RA   Fuller S., Flores C., Peschon J., Nester E., Gordon M.;
RT   "Nucleotide sequence of the tms genes of the pTiA6NC octopine Ti plasmid:
RT   two gene products involved in plant tumorigenesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:1728-1732(1984).
CC   -!- FUNCTION: Hydrolyzes indole-3-acetamide (IAM) into indole-3-acetic acid
CC       (IAA).
CC   -!- PATHWAY: Plant hormone metabolism; auxin biosynthesis.
CC   -!- SIMILARITY: Belongs to the amidase family. {ECO:0000305}.
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DR   EMBL; X00409; CAA25116.1; -; Genomic_DNA.
DR   EMBL; AF242881; AAF77122.1; -; Genomic_DNA.
DR   EMBL; K02553; AAA92549.1; -; Genomic_DNA.
DR   PIR; A04501; Q2AGAT.
DR   RefSeq; NP_059675.1; NC_002377.1.
DR   RefSeq; WP_010892363.1; NZ_QSNU01000012.1.
DR   AlphaFoldDB; P0A2X0; -.
DR   SMR; P0A2X0; -.
DR   OrthoDB; 1239251at2; -.
DR   BioCyc; MetaCyc:MON-7862; -.
DR   UniPathway; UPA00151; -.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009851; P:auxin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.90.1300.10; -; 1.
DR   InterPro; IPR000120; Amidase.
DR   InterPro; IPR020556; Amidase_CS.
DR   InterPro; IPR023631; Amidase_dom.
DR   InterPro; IPR036928; AS_sf.
DR   PANTHER; PTHR11895; PTHR11895; 1.
DR   Pfam; PF01425; Amidase; 1.
DR   SUPFAM; SSF75304; SSF75304; 1.
DR   PROSITE; PS00571; AMIDASES; 1.
PE   3: Inferred from homology;
KW   Auxin biosynthesis; Crown gall tumor; Hydrolase; Plasmid.
FT   CHAIN           1..467
FT                   /note="Indoleacetamide hydrolase"
FT                   /id="PRO_0000105241"
FT   ACT_SITE        74
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        149
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        173
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   467 AA;  49805 MW;  2E01153E3204C1DD CRC64;
     MVAITSLAQS LEHLKRKDYS CLELVETLIA RCEAAKSLNA LLATDWDGLR RSAKKIDRHG
     NAGVGLCGIP LCFKANIATG VFPTSAATPA LINHLPKIPS RVAERLFSAG ALPGASGNMH
     ELSFGITSNN YATGAVRNPW NPDLIPGGSS GGVAAAVASR LMLGGIGTDT GASVRLPAAL
     CGVVGFRPTL GRYPGDRIIP VSPTRDTPGI IAQCVADVVI LDRIISGTPE RIPPVPLKGL
     RIGLPTTYFY DDLDADVALA AETTIRLLAN KGVTFVEANI PHLDELNKGA SFPVALYEFP
     HALKQYLDDF VKTVSFSDVI KGIRSPDVAN IANAQIDGHQ ISKAEYELAR HSFRPRLQAT
     YRNYFKLNRL DAILFPTAPL VARPIGQDSS VIHNGTMLDT FKIYVRNVDP SSNAGLPGLS
     IPVCLTPDRL PVGMEIDGLA DSDQRLLAIG GALEEAIGFR YFAGLPN