ID   HYLP1_BPH44             Reviewed;         371 AA.
AC   P15316;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Hyaluronoglucosaminidase;
DE            Short=Hyaluronidase;
DE            EC=3.2.1.35;
GN   Name=HYLP1; Synonyms=HYLP;
OS   Streptococcus pyogenes phage H4489A.
OC   Viruses; unclassified bacterial viruses.
OX   NCBI_TaxID=12366;
OH   NCBI_TaxID=1314; Streptococcus pyogenes.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2643574; DOI=10.1128/iai.57.2.533-539.1989;
RA   Hynes W.L., Ferretti J.J.;
RT   "Sequence analysis and expression in Escherichia coli of the hyaluronidase
RT   gene of Streptococcus pyogenes bacteriophage H4489A.";
RL   Infect. Immun. 57:533-539(1989).
CC   -!- FUNCTION: The hyaluronidase of this bacteriophage mediates the
CC       penetration of the hyaluronic capsule of Streptococcus pyogenes.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC         glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 69 family. {ECO:0000305}.
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DR   EMBL; M19348; AAA98101.1; -; Genomic_DNA.
DR   PIR; A30566; HUBPHA.
DR   PDB; 6WV2; X-ray; 2.21 A; A/B/C=87-371.
DR   PDB; 6WWX; X-ray; 2.20 A; A/B/C=87-371.
DR   PDB; 6WXA; X-ray; 2.30 A; A/B/C=87-371.
DR   PDB; 6X3M; X-ray; 3.58 A; A/B/C/D/E/F/G/H/I=1-371.
DR   PDBsum; 6WV2; -.
DR   PDBsum; 6WWX; -.
DR   PDBsum; 6WXA; -.
DR   PDBsum; 6X3M; -.
DR   SMR; P15316; -.
DR   CAZy; PL16; Polysaccharide Lyase Family 16.
DR   BRENDA; 4.2.2.1; 7637.
DR   GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:InterPro.
DR   GO; GO:0098996; P:disruption of host cell glycocalyx during viral entry; IEA:UniProtKB-KW.
DR   InterPro; IPR009860; Hyaluronidase_bac.
DR   InterPro; IPR041352; Mtd_N.
DR   Pfam; PF07212; Hyaluronidase_1; 1.
DR   Pfam; PF18454; Mtd_N; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Degradation of host capsule during virus entry;
KW   Degradation of host cell envelope components during virus entry;
KW   Glycosidase; Hydrolase; Virion; Virus entry into host cell.
FT   CHAIN           1..371
FT                   /note="Hyaluronoglucosaminidase"
FT                   /id="PRO_0000057723"
FT   REGION          54..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           107..116
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   HELIX           120..122
FT                   /evidence="ECO:0007829|PDB:6WV2"
FT   HELIX           126..133
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          142..144
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          148..150
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          153..155
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   TURN            161..163
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          165..170
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          178..183
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          192..197
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          206..211
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          213..215
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          217..222
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          233..238
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          245..250
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          253..263
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          265..268
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   TURN            269..274
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          276..283
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   TURN            286..288
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          295..300
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          305..307
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          309..314
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          317..322
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          328..330
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   STRAND          334..338
FT                   /evidence="ECO:0007829|PDB:6WWX"
FT   HELIX           354..371
FT                   /evidence="ECO:0007829|PDB:6WWX"
SQ   SEQUENCE   371 AA;  39519 MW;  09D0DBC5F292B893 CRC64;
     MTENIPLRVQ FKRMSADEWA RSDVILLEGE IGFETDTGFA KFGDGQNTFS KLKYLTGPKG
     PKGDTGLQGK TGGTGPRGPA GKPGTTDYDQ LQNKPDLGAF AQKEETNSKI TKLESSKADK
     SAVYSKAESK IELDKKLSLT GGIVTGQLQF KPNKSGIKPS SSVGGAINID MSKSEGAAMV
     MYTNKDTTDG PLMILRSDKD TFDQSAQFVD YSGKTNAVNI VMRQPSAPNF SSALNITSAN
     EGGSAMQIRG VEKALGTLKI THENPNVEAK YDENAAALSI DIVKKQKGGK GTAAQGIYIN
     STSGTAGKML RIRNKNEDKF YVGPDGGFHS GANSTVAGNL TVKDPTSGKH AATKDYVDEK
     IAELKKLILK K