ID   HYLP2_BPH45             Reviewed;         337 AA.
AC   Q54699;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   29-SEP-2021, entry version 58.
DE   RecName: Full=Hyaluronoglucosaminidase;
DE            Short=Hyaluronidase;
DE            EC=3.2.1.35;
GN   Name=HYLP2;
OS   Streptococcus pyogenes phage H10403.
OC   Viruses; unclassified bacterial viruses.
OX   NCBI_TaxID=86065;
OH   NCBI_TaxID=1314; Streptococcus pyogenes.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7622224; DOI=10.1128/iai.63.8.3015-3020.1995;
RA   Hynes W.L., Hancock L., Ferretti J.J.;
RT   "Analysis of a second bacteriophage hyaluronidase gene from Streptococcus
RT   pyogenes: evidence for a third hyaluronidase involved in extracellular
RT   enzymatic activity.";
RL   Infect. Immun. 63:3015-3020(1995).
CC   -!- FUNCTION: The hyaluronidase of this bacteriophage mediates the
CC       penetration of the hyaluronic capsule of Streptococcus pyogenes.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC         glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 69 family. {ECO:0000305}.
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DR   EMBL; U28144; AAA86895.1; -; Genomic_DNA.
DR   SMR; Q54699; -.
DR   CAZy; PL16; Polysaccharide Lyase Family 16.
DR   PRIDE; Q54699; -.
DR   BRENDA; 3.2.1.36; 8503.
DR   SABIO-RK; Q54699; -.
DR   GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0045227; P:capsule polysaccharide biosynthetic process; IEA:InterPro.
DR   GO; GO:0098996; P:disruption of host cell glycocalyx during viral entry; IEA:UniProtKB-KW.
DR   InterPro; IPR009860; Hyaluronidase_bac.
DR   InterPro; IPR041352; Mtd_N.
DR   Pfam; PF07212; Hyaluronidase_1; 1.
DR   Pfam; PF18454; Mtd_N; 1.
PE   3: Inferred from homology;
KW   Degradation of host capsule during virus entry;
KW   Degradation of host cell envelope components during virus entry;
KW   Glycosidase; Hydrolase; Virion; Virus entry into host cell.
FT   CHAIN           1..337
FT                   /note="Hyaluronoglucosaminidase"
FT                   /id="PRO_0000057724"
SQ   SEQUENCE   337 AA;  36453 MW;  770EB8E6B7F9D842 CRC64;
     MSENIPLRVQ FKRMKAAEWA RSDVILLESE IGFETDTGFA RAGDGHNRFS DLGYISPLDY
     NLLTNKPNID GLATKVETAQ KLQQKADKET VYTKAESKQE LDKKLNLKGG VMTGQLKFKP
     AATVAYSSST GGAVNIDLSS TRGAGVVVYS DNDTSDGPLM SLRTGKETFN QSALFVDYKG
     TTNAVNIAMR HATTPNFSSA LNITSGNENG SAMQLRGSEK ALGTLKITHE NPSIGADYDK
     NAARYPLILS KRQNGAGTAA QGIYINSTSG TTGKLLRIRN LSDDKFYVKS DGGFYAKETS
     QIDGNLKLKD PTANDHAATK AYVDKAISEL KKLILKK