ID   HYPA2_BRADU             Reviewed;         113 AA.
AC   Q45256;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Hydrogenase maturation factor HypA 2 {ECO:0000255|HAMAP-Rule:MF_00213};
GN   Name=hypA2 {ECO:0000255|HAMAP-Rule:MF_00213}; Synonyms=hypA;
GN   OrderedLocusNames=bll6932;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=8305450; DOI=10.1016/0005-2728(94)90163-5;
RA   Fu C., Maier R.J.;
RT   "Nucleotide sequences of two hydrogenase-related genes (hypA and hypB) from
RT   Bradyrhizobium japonicum, one of which (hypB) encodes an extremely
RT   histidine-rich region and guanine nucleotide-binding domains.";
RL   Biochim. Biophys. Acta 1184:135-138(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_00213}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00213, ECO:0000305}.
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DR   EMBL; L24513; AAA17762.1; -; Unassigned_DNA.
DR   EMBL; BA000040; BAC52197.1; -; Genomic_DNA.
DR   RefSeq; NP_773572.1; NC_004463.1.
DR   RefSeq; WP_011089670.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q45256; -.
DR   SMR; Q45256; -.
DR   STRING; 224911.27355213; -.
DR   EnsemblBacteria; BAC52197; BAC52197; BAC52197.
DR   GeneID; 64026688; -.
DR   KEGG; bja:bll6932; -.
DR   PATRIC; fig|224911.44.peg.6966; -.
DR   eggNOG; COG0375; Bacteria.
DR   HOGENOM; CLU_126929_0_0_5; -.
DR   InParanoid; Q45256; -.
DR   OMA; ILLCPCG; -.
DR   PhylomeDB; Q45256; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0016151; F:nickel cation binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IBA:GO_Central.
DR   GO; GO:0051604; P:protein maturation; IBA:GO_Central.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   TIGRFAMs; TIGR00100; hypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Reference proteome; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HypA 2"
FT                   /id="PRO_0000129035"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         76
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
SQ   SEQUENCE   113 AA;  12331 MW;  5B2CFA98F1142601 CRC64;
     MHEMALCEGI IGIVEEEARK RAFAKVNVVC LEIGALSHVA PEALQFCFEA VAARTIAQGA
     KLEIVETPGT AWCMACSKSV EIKQRYEPCP SCGGYQLQVT GGEEMRVREL EVD