ID   HYPA_AZOCH              Reviewed;         113 AA.
AC   Q43948;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Hydrogenase maturation factor HypA {ECO:0000255|HAMAP-Rule:MF_00213};
DE   AltName: Full=Protein HupA;
GN   Name=hypA {ECO:0000255|HAMAP-Rule:MF_00213}; Synonyms=hupA;
OS   Azotobacter chroococcum mcd 1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8486288; DOI=10.1016/0378-1119(93)90616-b;
RA   Tibelius K.H., Du L., Tito D., Stejskal F.;
RT   "The Azotobacter chroococcum hydrogenase gene cluster: sequences and
RT   genetic analysis of four accessory genes, hupA, hupB, hupY and hupC.";
RL   Gene 127:53-61(1993).
RN   [2]
RP   ERRATUM OF PUBMED:8486288.
RA   Tibelius K.H., Du L., Tito D., Stejskal F.;
RL   Gene 134:141-141(1993).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_00213}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00213, ECO:0000305}.
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DR   EMBL; L00674; AAA22132.1; -; Genomic_DNA.
DR   PIR; JN0646; JN0646.
DR   AlphaFoldDB; Q43948; -.
DR   SMR; Q43948; -.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   TIGRFAMs; TIGR00100; hypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HypA"
FT                   /id="PRO_0000129032"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         76
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
SQ   SEQUENCE   113 AA;  12593 MW;  F55C86782BBF27D2 CRC64;
     MHEMSIAEGI VQLLEEQAAA QRFERVKAVW LEIGPLAAVE VESLRFCFEA VTRGSLAEGA
     RLEIVELPGR AWCLGCNASV AIRQRYDACP QCGSYRLQVT QGDELRVKEL EVE