ID   HYPA_CERSP              Reviewed;         113 AA.
AC   Q9REM0;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Hydrogenase maturation factor HypA {ECO:0000255|HAMAP-Rule:MF_00213};
GN   Name=hypA {ECO:0000255|HAMAP-Rule:MF_00213};
OS   Cereibacter sphaeroides (Rhodobacter sphaeroides).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Cereibacter.
OX   NCBI_TaxID=1063;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=601;
RA   Colbeau A., Chabert J., Wu J.-Q., Vignais P.M.;
RT   "Hup gene cluster from Rhodobacter sphaeroides.";
RL   Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=601;
RA   Xu D.Q., Wu Y.Q.;
RT   "Cloning of the hup gene cluster from Rhodobacter sphaeroides 601.";
RL   Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_00213}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00213, ECO:0000305}.
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DR   EMBL; AF214145; AAF19997.1; -; Genomic_DNA.
DR   EMBL; AJ315650; CAC42236.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9REM0; -.
DR   SMR; Q9REM0; -.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   TIGRFAMs; TIGR00100; hypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HypA"
FT                   /id="PRO_0000129048"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         76
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
SQ   SEQUENCE   113 AA;  12312 MW;  841858D2613E4312 CRC64;
     MHEMSLCEGI RGIVEDQARR HGFATVKVLR LEIGRFAGVE KAALGFAFDV VMRGSAAEGA
     RLEILELPGR ALCYDCGEEA AIQDRFDPCP LCGGGRLMPV GGDEMRIKDM EVQ