HYPA_CUPNH
ID HYPA_CUPNH Reviewed; 119 AA.
AC P31901; Q7WXU2;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Hydrogenase maturation factor HypA {ECO:0000255|HAMAP-Rule:MF_00213};
GN Name=hypA {ECO:0000255|HAMAP-Rule:MF_00213}; OrderedLocusNames=PHG012;
OS Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS / H16 / Stanier 337) (Ralstonia eutropha).
OG Plasmid megaplasmid pHG1.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Cupriavidus.
OX NCBI_TaxID=381666;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX PubMed=12948488; DOI=10.1016/s0022-2836(03)00894-5;
RA Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G.;
RT "Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid
RT encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis.";
RL J. Mol. Biol. 332:369-383(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 76-119.
RX PubMed=8352644; DOI=10.1007/bf00249034;
RA Dernedde J., Eitinger M., Friedrich B.;
RT "Analysis of a pleiotropic gene region involved in formation of
RT catalytically active hydrogenases in Alcaligenes eutrophus H16.";
RL Arch. Microbiol. 159:545-553(1993).
CC -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC for nickel insertion into the metal center of the hydrogenase.
CC {ECO:0000255|HAMAP-Rule:MF_00213}.
CC -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC Rule:MF_00213, ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY305378; AAP85768.1; -; Genomic_DNA.
DR EMBL; X70183; CAA49730.1; -; Genomic_DNA.
DR PIR; S29975; S29975.
DR RefSeq; WP_011153937.1; NZ_CP039289.1.
DR AlphaFoldDB; P31901; -.
DR SMR; P31901; -.
DR STRING; 381666.PHG012; -.
DR EnsemblBacteria; AAP85768; AAP85768; PHG012.
DR GeneID; 39976540; -.
DR KEGG; reh:PHG012; -.
DR eggNOG; COG0375; Bacteria.
DR HOGENOM; CLU_126929_0_0_4; -.
DR OMA; HEMTICE; -.
DR OrthoDB; 1995603at2; -.
DR Proteomes; UP000008210; Plasmid megaplasmid pHG1.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR HAMAP; MF_00213; HypA_HybF; 1.
DR InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR InterPro; IPR000688; HypA/HybF.
DR PANTHER; PTHR34535; PTHR34535; 1.
DR Pfam; PF01155; HypA; 1.
DR PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR PROSITE; PS01249; HYPA; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nickel; Plasmid; Reference proteome; Zinc.
FT CHAIN 1..119
FT /note="Hydrogenase maturation factor HypA"
FT /id="PRO_0000129030"
FT BINDING 2
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 73
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 76
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 89
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT BINDING 92
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
SQ SEQUENCE 119 AA; 12858 MW; 88D9397E2710E939 CRC64;
MHELSLAGGI LQLLEDAARR ERFARVTLLR LEAGKLCGVE VRALRFALEA IASGTCLEGA
CIEIEEPEGQ AWCLQCNAAV ALAERGAPCS GCGGYRLQPT AGTELRVMDM LVEDHWCPE
