HYPA_HELPY
ID HYPA_HELPY Reviewed; 117 AA.
AC P0A0U4; O25539;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Hydrogenase/urease maturation factor HypA {ECO:0000305};
GN Name=hypA {ECO:0000303|PubMed:11123699}; OrderedLocusNames=HP_0869;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 43504;
RX PubMed=11123699; DOI=10.1046/j.1365-2958.2001.02244.x;
RA Olson J.W., Mehta N.S., Maier R.J.;
RT "Requirement of nickel metabolism proteins HypA and HypB for full activity
RT of both hydrogenase and urease in Helicobacter pylori.";
RL Mol. Microbiol. 39:176-182(2001).
RN [3]
RP FUNCTION, SUBUNIT, INTERACTION WITH HYPB, NICKEL-BINDING, AND MUTAGENESIS
RP OF HIS-2; HIS-17; HIS-24; HIS-79 AND HIS-95.
RC STRAIN=ATCC 43504;
RX PubMed=12533448; DOI=10.1128/jb.185.3.726-734.2003;
RA Mehta N., Olson J.W., Maier R.J.;
RT "Characterization of Helicobacter pylori nickel metabolism accessory
RT proteins needed for maturation of both urease and hydrogenase.";
RL J. Bacteriol. 185:726-734(2003).
RN [4] {ECO:0007744|PDB:2KDX}
RP STRUCTURE BY NMR IN COMPLEX WITH ZINC, AND NICKEL-BINDING.
RX PubMed=19621959; DOI=10.1021/ja900543y;
RA Xia W., Li H., Sze K.H., Sun H.;
RT "Structure of a nickel chaperone, HypA, from Helicobacter pylori reveals
RT two distinct metal binding sites.";
RL J. Am. Chem. Soc. 131:10031-10040(2009).
CC -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC for nickel insertion into the metal center of the hydrogenase. Is also
CC required for urease maturation. {ECO:0000269|PubMed:11123699,
CC ECO:0000269|PubMed:12533448}.
CC -!- SUBUNIT: Monomer and homodimer. Forms a complex with HypB.
CC {ECO:0000269|PubMed:12533448}.
CC -!- DISRUPTION PHENOTYPE: Mutant has negligible hydrogenase activity and is
CC severely impaired in urease activity. {ECO:0000269|PubMed:11123699}.
CC -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC Rule:MF_00213, ECO:0000305}.
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DR EMBL; AE000511; AAD07910.1; -; Genomic_DNA.
DR PIR; E64628; E64628.
DR RefSeq; NP_207663.1; NC_000915.1.
DR RefSeq; WP_000545280.1; NC_018939.1.
DR PDB; 2KDX; NMR; -; A=1-117.
DR PDBsum; 2KDX; -.
DR AlphaFoldDB; P0A0U4; -.
DR BMRB; P0A0U4; -.
DR SMR; P0A0U4; -.
DR IntAct; P0A0U4; 19.
DR MINT; P0A0U4; -.
DR STRING; 85962.C694_04450; -.
DR PaxDb; P0A0U4; -.
DR DNASU; 899398; -.
DR EnsemblBacteria; AAD07910; AAD07910; HP_0869.
DR KEGG; hpy:HP_0869; -.
DR PATRIC; fig|85962.47.peg.923; -.
DR eggNOG; COG0375; Bacteria.
DR OMA; DCHQESE; -.
DR PhylomeDB; P0A0U4; -.
DR BioCyc; MetaCyc:HP_RS04240-MON; -.
DR EvolutionaryTrace; P0A0U4; -.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0016151; F:nickel cation binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IBA:GO_Central.
DR GO; GO:0051604; P:protein maturation; IBA:GO_Central.
DR HAMAP; MF_00213; HypA_HybF; 1.
DR InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR InterPro; IPR000688; HypA/HybF.
DR PANTHER; PTHR34535; PTHR34535; 1.
DR Pfam; PF01155; HypA; 1.
DR PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR TIGRFAMs; TIGR00100; hypA; 1.
DR PROSITE; PS01249; HYPA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Metal-binding; Nickel; Reference proteome; Zinc.
FT CHAIN 1..117
FT /note="Hydrogenase/urease maturation factor HypA"
FT /id="PRO_0000129042"
FT BINDING 2
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213,
FT ECO:0000305|PubMed:12533448, ECO:0000305|PubMed:19621959"
FT BINDING 3
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000305|PubMed:19621959"
FT BINDING 40
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000305|PubMed:19621959"
FT BINDING 74
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213,
FT ECO:0000269|PubMed:19621959, ECO:0007744|PDB:2KDX"
FT BINDING 77
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213,
FT ECO:0000269|PubMed:19621959, ECO:0007744|PDB:2KDX"
FT BINDING 91
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213,
FT ECO:0000269|PubMed:19621959, ECO:0007744|PDB:2KDX"
FT BINDING 94
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00213,
FT ECO:0000269|PubMed:19621959, ECO:0007744|PDB:2KDX"
FT MUTAGEN 2
FT /note="H->A: Cannot bind nickel. Lacks hydrogenase activity
FT and has only 2% of the urease activity."
FT /evidence="ECO:0000269|PubMed:12533448"
FT MUTAGEN 17
FT /note="H->A: Does not affect nickel-binding ability."
FT /evidence="ECO:0000269|PubMed:12533448"
FT MUTAGEN 24
FT /note="H->A: Does not affect nickel-binding ability."
FT /evidence="ECO:0000269|PubMed:12533448"
FT MUTAGEN 79
FT /note="H->A: Does not affect nickel-binding ability."
FT /evidence="ECO:0000269|PubMed:12533448"
FT MUTAGEN 95
FT /note="H->A: Does not affect nickel-binding ability."
FT /evidence="ECO:0000269|PubMed:12533448"
FT HELIX 2..20
FT /evidence="ECO:0007829|PDB:2KDX"
FT STRAND 28..34
FT /evidence="ECO:0007829|PDB:2KDX"
FT HELIX 41..51
FT /evidence="ECO:0007829|PDB:2KDX"
FT HELIX 52..54
FT /evidence="ECO:0007829|PDB:2KDX"
FT TURN 56..58
FT /evidence="ECO:0007829|PDB:2KDX"
FT STRAND 63..68
FT /evidence="ECO:0007829|PDB:2KDX"
FT STRAND 71..73
FT /evidence="ECO:0007829|PDB:2KDX"
FT STRAND 75..78
FT /evidence="ECO:0007829|PDB:2KDX"
FT STRAND 92..96
FT /evidence="ECO:0007829|PDB:2KDX"
FT STRAND 100..105
FT /evidence="ECO:0007829|PDB:2KDX"
FT STRAND 108..113
FT /evidence="ECO:0007829|PDB:2KDX"
SQ SEQUENCE 117 AA; 13202 MW; BA67A69E2EC35506 CRC64;
MHEYSVVSSL IALCEEHAKK NQAHKIERVV VGIGERSAMD KSLFVSAFET FREESLVCKD
AILDIVDEKV ELECKDCSHV FKPNALDYGV CEKCHSKNVI ITQGNEMRLL SLEMLAE
