ID   HYPA_NOSP7              Reviewed;         113 AA.
AC   Q9EYH3; B2J5X4;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Hydrogenase maturation factor HypA {ECO:0000255|HAMAP-Rule:MF_00213};
GN   Name=hypA {ECO:0000255|HAMAP-Rule:MF_00213}; OrderedLocusNames=Npun_R0358;
OS   Nostoc punctiforme (strain ATCC 29133 / PCC 73102).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=63737;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Wunschiers R., Lindblad P.;
RT   "Organization of hyp-genes in the cyanobacterium Nostoc PCC73102.";
RL   Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29133 / PCC 73102;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C., Elhai J.,
RA   Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.;
RT   "Complete sequence of chromosome of Nostoc punctiforme ATCC 29133.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_00213}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00213, ECO:0000305}.
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DR   EMBL; AF325724; AAG49380.1; -; Genomic_DNA.
DR   EMBL; CP001037; ACC79140.1; -; Genomic_DNA.
DR   RefSeq; WP_012407166.1; NC_010628.1.
DR   AlphaFoldDB; Q9EYH3; -.
DR   SMR; Q9EYH3; -.
DR   STRING; 63737.Npun_R0358; -.
DR   DNASU; 6249826; -.
DR   EnsemblBacteria; ACC79140; ACC79140; Npun_R0358.
DR   KEGG; npu:Npun_R0358; -.
DR   eggNOG; COG0375; Bacteria.
DR   HOGENOM; CLU_126929_3_0_3; -.
DR   OMA; ILLCPCG; -.
DR   OrthoDB; 1995603at2; -.
DR   PhylomeDB; Q9EYH3; -.
DR   Proteomes; UP000001191; Chromosome.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Reference proteome; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HypA"
FT                   /id="PRO_0000129044"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         70
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         86
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         88
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
SQ   SEQUENCE   113 AA;  12363 MW;  C6F668E5AC8BC59B CRC64;
     MHEFGITQNI VAIVTEHAKG AKVQRVLLEI GKLSAIMPDA IRFCFDICTQ GTVLEGATLE
     ILEIPGLGRC RQCGGEIYLD KPFGICNCGS VQLDLITGEE LKIKEIEIEE LCV