ID   HYPA_RHILV              Reviewed;         113 AA.
AC   P28154;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Hydrogenase maturation factor HypA {ECO:0000255|HAMAP-Rule:MF_00213};
DE   AltName: Full=Protein HupL;
GN   Name=hypA {ECO:0000255|HAMAP-Rule:MF_00213}; Synonyms=hupL;
OS   Rhizobium leguminosarum bv. viciae.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium.
OX   NCBI_TaxID=387;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=128c53;
RX   PubMed=8326860; DOI=10.1111/j.1365-2958.1993.tb01591.x;
RA   Rey L., Murillo J., Hernando Y., Hidalgo E., Cabrera E., Imperial J.,
RA   Ruiz-Argueso T.;
RT   "Molecular analysis of a microaerobically induced operon required for
RT   hydrogenase synthesis in Rhizobium leguminosarum biovar viciae.";
RL   Mol. Microbiol. 8:471-481(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=B10;
RA   Brito B., Palacios J.M., Imperial J., Ruiz-Argueso T., Yang W.C.,
RA   Bisseling T., Schmitt H., Kerl V., Bauer T., Kokotek W., Lotz W.;
RT   "Organization of the hup-region and its differential transcription in non-
RT   symbiotic and symbiotic cells of Rhizobium leguminosarum bv. viciae B10.";
RL   Mol. Plant Microbe Interact. 8:235-240(1997).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase.
CC       {ECO:0000255|HAMAP-Rule:MF_00213}.
CC   -!- SIMILARITY: Belongs to the HypA/HybF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00213, ECO:0000305}.
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DR   EMBL; X52974; CAA37159.1; -; Genomic_DNA.
DR   EMBL; Z36981; CAA85441.1; -; Genomic_DNA.
DR   PIR; S32873; S32873.
DR   RefSeq; WP_026242366.1; NZ_WIEG01000075.1.
DR   AlphaFoldDB; P28154; -.
DR   SMR; P28154; -.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   HAMAP; MF_00213; HypA_HybF; 1.
DR   InterPro; IPR020538; Hydgase_Ni_incorp_HypA/HybF_CS.
DR   InterPro; IPR000688; HypA/HybF.
DR   PANTHER; PTHR34535; PTHR34535; 1.
DR   Pfam; PF01155; HypA; 1.
DR   PIRSF; PIRSF004761; Hydrgn_mat_HypA; 1.
DR   TIGRFAMs; TIGR00100; hypA; 1.
DR   PROSITE; PS01249; HYPA; 1.
PE   3: Inferred from homology;
KW   Metal-binding; Nickel; Zinc.
FT   CHAIN           1..113
FT                   /note="Hydrogenase maturation factor HypA"
FT                   /id="PRO_0000129045"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         73
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         76
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         89
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
FT   BINDING         92
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00213"
SQ   SEQUENCE   113 AA;  12447 MW;  F657134559CDAC67 CRC64;
     MHEMSLMESV IEIVCETARQ NGATRVKSVR LDVGVLSHVN PDALIFCYEA VRHGTVADSA
     TLEINRIAGE GWCLDCGKTV ALEERFGACP DCGRHRVQMT AGDELKIRDM EVI