ID   HYPB_AZOVI              Reviewed;         303 AA.
AC   P31880;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Hydrogenase maturation factor HypB {ECO:0000250|UniProtKB:P0AAN3};
GN   Name=hypB;
OS   Azotobacter vinelandii.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Azotobacter.
OX   NCBI_TaxID=354;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW;
RX   PubMed=1610901; DOI=10.1016/0167-4781(92)90077-d;
RA   Chen J.C., Mortenson L.E.;
RT   "Identification of six open reading frames from a region of the Azotobacter
RT   vinelandii genome likely involved in dihydrogen metabolism.";
RL   Biochim. Biophys. Acta 1131:199-202(1992).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase. Exhibits
CC       a low intrinsic GTPase activity, which is essential for nickel
CC       insertion. {ECO:0000250|UniProtKB:P0AAN3}.
CC   -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. HypB/HupM
CC       subfamily. {ECO:0000305}.
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DR   EMBL; X63650; CAA45184.1; -; Genomic_DNA.
DR   EMBL; L23970; AAA19509.1; -; Unassigned_DNA.
DR   PIR; S23440; S23440.
DR   RefSeq; WP_012703489.1; NZ_FPKM01000029.1.
DR   AlphaFoldDB; P31880; -.
DR   SMR; P31880; -.
DR   OMA; GRSCHLD; -.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   CDD; cd05390; HypB; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003495; CobW/HypB/UreG_dom.
DR   InterPro; IPR004392; Hyd_mat_HypB.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR30134; PTHR30134; 1.
DR   Pfam; PF02492; cobW; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00073; hypB; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Hydrolase; Metal-binding; Nickel; Nucleotide-binding; Zinc.
FT   CHAIN           1..303
FT                   /note="Hydrogenase maturation factor HypB"
FT                   /id="PRO_0000201436"
FT   REGION          18..63
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          99..260
FT                   /note="G-domain"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         5
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         7
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         159
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         159
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         160
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         160
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         191
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         191
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
SQ   SEQUENCE   303 AA;  33186 MW;  743E6775527D1922 CRC64;
     MCTVCGCAEG ETRIEGEHHH HGHDHHHHEH PFVRRPAPAE AAPPAAGGPN LHFGQGPARA
     HAPGLSQSRM LRIEQDILGK NDRYAAENRA RFEALSLFVL NLVSSPGSGK TSLLTRTIEL
     LGRRRPLAVI EGDQQTDHDA ARIRATGVPA VQINTGKGCH LDAHMVGHAL ARLDDLEGGL
     LFIENVGNLV CPAAFDLGEA HKVAILSVTE GEDKPLKYPD MFHAADLMLL NKTDLLPHLD
     FDVEACIAYA RRVNPDIEVI RVSARSGEGM GEWLAWIERQ RGTRLRARID ALREQARALE
     ALL