ID   HYPB_ECOL6              Reviewed;         290 AA.
AC   P0AAN4; P24190; Q46884;
DT   11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Hydrogenase maturation factor HypB {ECO:0000250|UniProtKB:P0AAN3};
GN   Name=hypB; OrderedLocusNames=c3287;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase. Exhibits
CC       a low intrinsic GTPase activity, which is essential for nickel
CC       insertion. {ECO:0000250|UniProtKB:P0AAN3}.
CC   -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. HypB/HupM
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE014075; AAN81736.1; -; Genomic_DNA.
DR   RefSeq; WP_000337665.1; NC_004431.1.
DR   AlphaFoldDB; P0AAN4; -.
DR   SMR; P0AAN4; -.
DR   STRING; 199310.c3287; -.
DR   EnsemblBacteria; AAN81736; AAN81736; c3287.
DR   GeneID; 66673404; -.
DR   KEGG; ecc:c3287; -.
DR   eggNOG; COG0378; Bacteria.
DR   HOGENOM; CLU_056148_1_0_6; -.
DR   OMA; ECHLDAH; -.
DR   BioCyc; ECOL199310:C3287-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   CDD; cd05390; HypB; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003495; CobW/HypB/UreG_dom.
DR   InterPro; IPR004392; Hyd_mat_HypB.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR30134; PTHR30134; 1.
DR   Pfam; PF02492; cobW; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00073; hypB; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Hydrolase; Metal-binding; Nickel; Nucleotide-binding; Zinc.
FT   CHAIN           1..290
FT                   /note="Hydrogenase maturation factor HypB"
FT                   /id="PRO_0000201441"
FT   REGION          106..267
FT                   /note="G-domain"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         2
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         5
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         7
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         166
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         166
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         167
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         167
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         198
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         198
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
SQ   SEQUENCE   290 AA;  31565 MW;  5B3AEB59AA6915C3 CRC64;
     MCTTCGCGEG NLYIEGDEHN PHSAFRSAPF APAARPKMKI TGIKAPEFTP SQTEEGDLHY
     GHGEAGTHAP GMSQRRMLEV EIDVLDKNNR LAERNRARFA ARKQLVLNLV SSPGSGKTTL
     LTETLMRLKD SVPCAVIEGD QQTVNDAARI RATGTPAIQV NTGKGCHLDA QMIADAAPRL
     PLDDNGILFI ENVGNLVCPA SFDLGEKHKV AVLSVTEGED KPLKYPHMFA AASLMLLNKV
     DLLPYLNFDV EKCIACAREV NPEIEIILIS ATSGEGMDQW LNWLETQRCA