ID   HYPB_HELHP              Reviewed;         242 AA.
AC   Q7VJB8;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Hydrogenase/urease maturation factor HypB {ECO:0000250|UniProtKB:O25560};
GN   Name=hypB; OrderedLocusNames=HH_0325;
OS   Helicobacter hepaticus (strain ATCC 51449 / 3B1).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=235279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51449 / 3B1;
RX   PubMed=12810954; DOI=10.1073/pnas.1332093100;
RA   Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M.,
RA   Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R.,
RA   Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B.,
RA   Shen Z., Weber J., Frosch M., Fox J.G.;
RT   "The complete genome sequence of the carcinogenic bacterium Helicobacter
RT   hepaticus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 51449 / 3B1;
RX   PubMed=17975083; DOI=10.1099/mic.0.2007/010520-0;
RA   Benoit S.L., Zbell A.L., Maier R.J.;
RT   "Nickel enzyme maturation in Helicobacter hepaticus: roles of accessory
RT   proteins in hydrogenase and urease activities.";
RL   Microbiology 153:3748-3756(2007).
CC   -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC       for nickel insertion into the metal center of the hydrogenase. Exhibits
CC       a low intrinsic GTPase activity, which is essential for nickel
CC       insertion. {ECO:0000250|UniProtKB:P0AAN3}.
CC   -!- DISRUPTION PHENOTYPE: Strains missing this gene do not produce
CC       hydrogenase or urease; addition of Ni(2+) to cell cultures compensates
CC       only poorly for this loss of function. Normal amounts of apo-urease are
CC       produced by the cells. {ECO:0000269|PubMed:17975083}.
CC   -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. HypB/HupM
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE017125; AAP76922.1; -; Genomic_DNA.
DR   RefSeq; WP_011115168.1; NC_004917.1.
DR   AlphaFoldDB; Q7VJB8; -.
DR   SMR; Q7VJB8; -.
DR   STRING; 235279.HH_0325; -.
DR   EnsemblBacteria; AAP76922; AAP76922; HH_0325.
DR   KEGG; hhe:HH_0325; -.
DR   eggNOG; COG0378; Bacteria.
DR   HOGENOM; CLU_056148_0_1_7; -.
DR   OMA; ECHLDAH; -.
DR   OrthoDB; 1134430at2; -.
DR   Proteomes; UP000002495; Chromosome.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0051604; P:protein maturation; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003495; CobW/HypB/UreG_dom.
DR   InterPro; IPR004392; Hyd_mat_HypB.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR30134; PTHR30134; 1.
DR   Pfam; PF02492; cobW; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00073; hypB; 1.
PE   3: Inferred from homology;
KW   GTP-binding; Hydrolase; Metal-binding; Nickel; Nickel insertion;
KW   Nucleotide-binding; Reference proteome; Zinc.
FT   CHAIN           1..242
FT                   /note="Hydrogenase/urease maturation factor HypB"
FT                   /id="PRO_0000372090"
FT   REGION          48..211
FT                   /note="G-domain"
FT                   /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT   BINDING         106
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250|UniProtKB:O25560"
FT   BINDING         106
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:O25560"
FT   BINDING         107
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250|UniProtKB:O25560"
FT   BINDING         142
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000250|UniProtKB:O25560"
FT   BINDING         142
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:O25560"
SQ   SEQUENCE   242 AA;  27328 MW;  B9E818F3EED77C5B CRC64;
     MNLSRNERLE NNPNLNKKSI QIVSKILSQN DLKAQELREK YKQLGVYVIN LMSSPGSGKT
     TLLESLSAFK DFTFCVLEGD LQTNRDAQRL EAKGVSAYQI TTGEACHLEA SMIETALCEL
     QKQCDMQQMD YLFIENVGNL VCPASYDVGA SLNIVLLSTP EGDDKILKYP TMFMCADAVI
     VSKADMMQYF GFSLTRVKED LGQLKANVPL FLTSNKERES IAKVRDFIEQ KRAQNYQSHH
     QF