HYPB_METJA
ID HYPB_METJA Reviewed; 221 AA.
AC Q57884;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Probable hydrogenase maturation factor HypB {ECO:0000305};
GN Name=hypB {ECO:0000303|PubMed:16807243}; OrderedLocusNames=MJ0442;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2] {ECO:0007744|PDB:2HF8, ECO:0007744|PDB:2HF9}
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH ZINC, AND SUBUNIT.
RX PubMed=16807243; DOI=10.1074/jbc.m600809200;
RA Gasper R., Scrima A., Wittinghofer A.;
RT "Structural insights into HypB, a GTP-binding protein that regulates metal
RT binding.";
RL J. Biol. Chem. 281:27492-27502(2006).
CC -!- FUNCTION: Involved in the maturation of [NiFe] hydrogenases. Required
CC for nickel insertion into the metal center of the hydrogenase. Exhibits
CC a low intrinsic GTPase activity, which is essential for nickel
CC insertion. {ECO:0000250|UniProtKB:P0AAN3}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:16807243}.
CC -!- SIMILARITY: Belongs to the SIMIBI class G3E GTPase family. HypB/HupM
CC subfamily. {ECO:0000305}.
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DR EMBL; L77117; AAB98429.1; -; Genomic_DNA.
DR PIR; B64355; B64355.
DR RefSeq; WP_010869941.1; NC_000909.1.
DR PDB; 2HF8; X-ray; 2.10 A; A/B=1-221.
DR PDB; 2HF9; X-ray; 1.90 A; A/B=1-221.
DR PDBsum; 2HF8; -.
DR PDBsum; 2HF9; -.
DR AlphaFoldDB; Q57884; -.
DR SMR; Q57884; -.
DR STRING; 243232.MJ_0442; -.
DR EnsemblBacteria; AAB98429; AAB98429; MJ_0442.
DR GeneID; 1451302; -.
DR KEGG; mja:MJ_0442; -.
DR eggNOG; arCOG01231; Archaea.
DR HOGENOM; CLU_056148_0_1_2; -.
DR InParanoid; Q57884; -.
DR OMA; ECHLDAH; -.
DR OrthoDB; 76245at2157; -.
DR PhylomeDB; Q57884; -.
DR EvolutionaryTrace; Q57884; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0016151; F:nickel cation binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IBA:GO_Central.
DR GO; GO:0051604; P:protein maturation; IBA:GO_Central.
DR CDD; cd05390; HypB; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003495; CobW/HypB/UreG_dom.
DR InterPro; IPR004392; Hyd_mat_HypB.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR30134; PTHR30134; 1.
DR Pfam; PF02492; cobW; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00073; hypB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; GTP-binding; Hydrolase; Metal-binding; Nickel;
KW Nucleotide-binding; Reference proteome; Zinc.
FT CHAIN 1..221
FT /note="Probable hydrogenase maturation factor HypB"
FT /id="PRO_0000201445"
FT REGION 35..196
FT /note="G-domain"
FT /evidence="ECO:0000250|UniProtKB:P0AAN3"
FT BINDING 95
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250|UniProtKB:O25560"
FT BINDING 95
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000269|PubMed:16807243,
FT ECO:0007744|PDB:2HF8"
FT BINDING 96
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250|UniProtKB:O25560"
FT BINDING 96
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000269|PubMed:16807243,
FT ECO:0007744|PDB:2HF8"
FT BINDING 100
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000269|PubMed:16807243,
FT ECO:0007744|PDB:2HF8, ECO:0007744|PDB:2HF9"
FT BINDING 104
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000269|PubMed:16807243,
FT ECO:0007744|PDB:2HF8, ECO:0007744|PDB:2HF9"
FT BINDING 127
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000250|UniProtKB:O25560"
FT BINDING 127
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000269|PubMed:16807243,
FT ECO:0007744|PDB:2HF8"
FT HELIX 13..30
FT /evidence="ECO:0007829|PDB:2HF9"
FT STRAND 34..41
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 46..57
FT /evidence="ECO:0007829|PDB:2HF9"
FT TURN 58..60
FT /evidence="ECO:0007829|PDB:2HF9"
FT STRAND 63..69
FT /evidence="ECO:0007829|PDB:2HF9"
FT TURN 70..72
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 73..80
FT /evidence="ECO:0007829|PDB:2HF9"
FT TURN 81..83
FT /evidence="ECO:0007829|PDB:2HF9"
FT STRAND 85..90
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 99..106
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 111..113
FT /evidence="ECO:0007829|PDB:2HF9"
FT STRAND 115..120
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 127..130
FT /evidence="ECO:0007829|PDB:2HF9"
FT STRAND 136..143
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:2HF9"
FT TURN 148..153
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 155..158
FT /evidence="ECO:0007829|PDB:2HF9"
FT STRAND 162..167
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 169..171
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 172..175
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 179..189
FT /evidence="ECO:0007829|PDB:2HF9"
FT STRAND 193..197
FT /evidence="ECO:0007829|PDB:2HF9"
FT TURN 200..202
FT /evidence="ECO:0007829|PDB:2HF9"
FT HELIX 206..219
FT /evidence="ECO:0007829|PDB:2HF9"
SQ SEQUENCE 221 AA; 24340 MW; 0BB5415386A81138 CRC64;
MHLVGVLDIA KDILKANKRL ADKNRKLLNK HGVVAFDFMG AIGSGKTLLI EKLIDNLKDK
YKIACIAGDV IAKFDAERME KHGAKVVPLN TGKECHLDAH LVGHALEDLN LDEIDLLFIE
NVGNLICPAD FDLGTHKRIV VISTTEGDDT IEKHPGIMKT ADLIVINKID LADAVGADIK
KMENDAKRIN PDAEVVLLSL KTMEGFDKVL EFIEKSVKEV K
