ID   HYSA_CUTAC              Reviewed;         812 AA.
AC   P0CZ00; Q59634; Q6AAT4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Hyaluronate lyase {ECO:0000303|PubMed:9115089};
DE            EC=4.2.2.1 {ECO:0000250|UniProtKB:Q54873};
DE   AltName: Full=Hyaluronidase {ECO:0000303|PubMed:9115089};
DE            Short=HYase;
DE   Flags: Precursor;
OS   Cutibacterium acnes (Propionibacterium acnes).
OC   Bacteria; Actinobacteria; Propionibacteriales; Propionibacteriaceae;
OC   Cutibacterium.
OX   NCBI_TaxID=1747;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=49/51;
RX   PubMed=9115089; DOI=10.1139/m97-044;
RA   Steiner B.M., Romero-Steiner S., Cruce D., George R.;
RT   "Cloning and sequencing of the hyaluronate lyase gene from
RT   Propionibacterium acnes.";
RL   Can. J. Microbiol. 43:315-321(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[hyaluronan](n) = n 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-
CC         acetyl-D-glucosamine + H2O; Xref=Rhea:RHEA:50240, Rhea:RHEA-
CC         COMP:12583, ChEBI:CHEBI:15377, ChEBI:CHEBI:132151,
CC         ChEBI:CHEBI:132153; EC=4.2.2.1;
CC         Evidence={ECO:0000250|UniProtKB:Q54873};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:9115089}.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of the
CC       signal peptide cleavage has been experimentally proven.
CC       {ECO:0000255|PROSITE-ProRule:PRU00648, ECO:0000269|PubMed:9115089}.
CC   -!- SIMILARITY: Belongs to the polysaccharide lyase 8 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA51650.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U15927; AAA51650.1; ALT_FRAME; Genomic_DNA.
DR   AlphaFoldDB; P0CZ00; -.
DR   SMR; P0CZ00; -.
DR   CAZy; PL8; Polysaccharide Lyase Family 8.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0030340; F:hyaluronate lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 1.50.10.100; -; 1.
DR   Gene3D; 2.60.220.10; -; 1.
DR   Gene3D; 2.70.98.10; -; 1.
DR   InterPro; IPR008929; Chondroitin_lyas.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR038970; Lyase_8.
DR   InterPro; IPR011071; Lyase_8-like_C.
DR   InterPro; IPR012970; Lyase_8_alpha_N.
DR   InterPro; IPR003159; Lyase_8_central_dom.
DR   InterPro; IPR006311; TAT_signal.
DR   PANTHER; PTHR38481; PTHR38481; 1.
DR   Pfam; PF02278; Lyase_8; 1.
DR   Pfam; PF08124; Lyase_8_N; 1.
DR   SUPFAM; SSF48230; SSF48230; 1.
DR   SUPFAM; SSF49863; SSF49863; 1.
DR   SUPFAM; SSF74650; SSF74650; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Lyase; Secreted; Signal.
FT   SIGNAL          1..32
FT                   /note="Tat-type signal"
FT                   /evidence="ECO:0000269|PubMed:9115089"
FT   CHAIN           33..812
FT                   /note="Hyaluronate lyase"
FT                   /id="PRO_0000410486"
FT   ACT_SITE        222
FT                   /evidence="ECO:0000250|UniProtKB:Q54873"
FT   ACT_SITE        272
FT                   /evidence="ECO:0000250|UniProtKB:Q54873"
FT   ACT_SITE        281
FT                   /evidence="ECO:0000250|UniProtKB:Q54873"
SQ   SEQUENCE   812 AA;  87758 MW;  D72FB62E1439CB96 CRC64;
     MFGTPSRRTF LTASALSAMA LAASPTVTDA IAAPGPDSWS ALCERWIDII TGRRAARTSD
     PRARAIIAKT DRKVAEILTD LVSGSSRQTV LISADLRKEQ SPFITKTARA IESMACGWAT
     PGSSYHKDPE ILSACIEGLR DFCRLRYNPS QDEYGNWWDW EDGASRAVAD VMCILHDVLP
     PEVMSAAAAG IDHFIPDPWF QQPGSVKPTA NPVQPVVSTG ANRMDLTRAV MCRSIATGDE
     KRLRHAVDGL PDAWRVTTEG DGFRADGGFI QHSHIPYTGG YGDVLFSGLA MLFPLVSGMR
     FDIDESARKA FHDQVERGFI PVMYNGQILD DVRGRSISRI NESAAMHGIS IARAMLMMAD
     ALPTHRAEQW RGIVHGWMAR NTFDHLSEPS TLVDISLFDA AAKAPRPGVV DAELLRVHGP
     SRPATADWLI TVSNCSDRIA WYEYGNGENE WAYRTSQGMR YLLLPGDMGQ YEDGYWATVD
     YSAPTGTTVD STPLKRAVGA SWAAKTPTNE WSGGLASGSW SAAASHITSQ DSALKARRLW
     VGLKDAMVEL TTDVTTDASR AITVVEHRKV ASSSTKLLVD GNRVSSATSF QNPRWAHLDG
     VGGYVFATDT DLSADVATRK GTWIDVNPSR KVKGADEVIE RAYASLHGHP PRSSSPWALL
     PTASRSHTMA LATRPGVEPF TVLRNDGNRP GRASAGALLT KDPTVVTTLA FWKPATCGGV
     AVNRPALVQT RESANQMEVV IVEPTQKRGS LTVTIEGSWK VKTADSHVDV SCENAAGTLH
     VDTAGLGGQS VRVTLARQVT QTPSGGGRHD RA