ID   HYUA_PSESN              Reviewed;         690 AA.
AC   Q01262; O70104;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=Putative D-/L-hydantoinase subunit A {ECO:0000305|PubMed:1732229};
DE            EC=3.5.2.- {ECO:0000305|PubMed:1732229};
DE   AltName: Full=Hydantoin utilization protein A {ECO:0000303|PubMed:1732229};
GN   Name=hyuA {ECO:0000303|PubMed:1732229};
OS   Pseudomonas sp. (strain NS671).
OG   Plasmid pHN671.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=29441;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=NS671;
RX   PubMed=1732229; DOI=10.1128/jb.174.3.962-969.1992;
RA   Watabe K.;
RT   "Cloning and sequencing of the genes involved in the conversion of 5-
RT   substituted hydantoins to the corresponding L-amino acids from the native
RT   plasmid of Pseudomonas sp. strain NS671.";
RL   J. Bacteriol. 174:962-969(1992).
CC   -!- FUNCTION: Involved in the asymmetric conversion of racemic 5-
CC       substituted hydantoins to the corresponding L-amino acids. HyuA and
CC       HyuB are both required for the conversion of D- and L-5-substituted
CC       hydantoins to corresponding N-carbamoyl-D- and N-carbamoyl-L-amino
CC       acids, respectively. {ECO:0000269|PubMed:1732229}.
CC   -!- SUBUNIT: May form a complex with HyuB. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the HyuA family. {ECO:0000305}.
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DR   EMBL; M72717; AAA25845.1; -; Genomic_DNA.
DR   EMBL; D10494; BAA01377.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q01262; -.
DR   SMR; Q01262; -.
DR   GO; GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IDA:UniProtKB.
DR   GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR008040; Hydant_A_N.
DR   InterPro; IPR002821; Hydantoinase_A.
DR   InterPro; IPR045079; Oxoprolinase_fam.
DR   PANTHER; PTHR11365; PTHR11365; 1.
DR   Pfam; PF05378; Hydant_A_N; 1.
DR   Pfam; PF01968; Hydantoinase_A; 1.
DR   SUPFAM; SSF53067; SSF53067; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Hydrolase; Plasmid.
FT   CHAIN           1..690
FT                   /note="Putative D-/L-hydantoinase subunit A"
FT                   /id="PRO_0000208580"
SQ   SEQUENCE   690 AA;  75622 MW;  CC7C0552A12DECA4 CRC64;
     MKLFGVDVGG TFTDIIFSDT ETRVTAIHKV PTTLDDPSTG VVQGILELCD RQYIDRTAID
     HVFHGTTIAT NAILEYDGAK TGMITTEGYR DIIHIGRHQR PQNYSIMQEI PWQDRPLVQR
     RHRLAIAERM GPVKGQVITP VQEDQVRGAV ATLKERGVDS IIVNFLFSYT NPEHEQRVKE
     IIEEEYPEAF VTISSEVSPQ FREFERFTTA SINGFVGPKV KNYIQNLEQS LKDSGISAEL
     HIMCSNGGVA TPKTVSEKPV NTLLSGPAAG ILGGAWAGEL TNRQKLITFD VGGTSADIGI
     ITDSGYGESS ARDTWIAGYP VMVPMIDIHT IGAGGGSIAH IDEGGAFKVG PRSAGSRPGP
     ACYGHGGLKP TVSDANVVLG RIDEHNFLGG EMKIYTNAAY KVIDELAGQL DLSRERTAEG
     VLQIMNNNMA NAIREKTIQK GEDPREFSLV AFGGAGPLHA VEVAQILNIP EVIIPLYPGI
     NSATGLLTTD LKYDVIKTEF MMSTNMDFSG LNEDLAGLET QLINQLKEDG VSKQDIRILR
     SADCRYAGQG YELRVDLPDV FLDEETIVDA LNNFHESHKA EYGHNFTDSP IEFVNIRVTG
     TGYMPKIEKQ AIHHEYQLED ALLKTGDATF NIDGSLVKVE INFYQREKIP VGAEFNGPCI
     VLQKDTTTVI PPNCTAYIEE YGNMIIKVGV