ID   HYUB_PSESN              Reviewed;         592 AA.
AC   Q01263; O70096;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Putative D-/L-hydantoinase subunit B {ECO:0000305|PubMed:1732229};
DE            EC=3.5.2.- {ECO:0000305|PubMed:1732229};
DE   AltName: Full=Hydantoin utilization protein B {ECO:0000303|PubMed:1732229};
GN   Name=hyuB {ECO:0000303|PubMed:1732229};
OS   Pseudomonas sp. (strain NS671).
OG   Plasmid pHN671.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=29441;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=NS671;
RX   PubMed=1732229; DOI=10.1128/jb.174.3.962-969.1992;
RA   Watabe K.;
RT   "Cloning and sequencing of the genes involved in the conversion of 5-
RT   substituted hydantoins to the corresponding L-amino acids from the native
RT   plasmid of Pseudomonas sp. strain NS671.";
RL   J. Bacteriol. 174:962-969(1992).
CC   -!- FUNCTION: Involved in the asymmetric conversion of racemic 5-
CC       substituted hydantoins to the corresponding L-amino acids. HyuA and
CC       HyuB are both required for the conversion of D- and L-5-substituted
CC       hydantoins to corresponding N-carbamoyl-D- and N-carbamoyl-L-amino
CC       acids, respectively. {ECO:0000269|PubMed:1732229}.
CC   -!- SUBUNIT: May form a complex with HyuA. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the HyuB family. {ECO:0000305}.
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DR   EMBL; M72717; AAA25846.1; -; Genomic_DNA.
DR   EMBL; D10494; BAA01378.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q01263; -.
DR   SMR; Q01263; -.
DR   GO; GO:0016810; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; IDA:UniProtKB.
DR   GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003692; Hydantoinase_B.
DR   InterPro; IPR045079; Oxoprolinase_fam.
DR   PANTHER; PTHR11365; PTHR11365; 1.
DR   Pfam; PF02538; Hydantoinase_B; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Hydrolase; Plasmid.
FT   CHAIN           1..592
FT                   /note="Putative D-/L-hydantoinase subunit B"
FT                   /id="PRO_0000208581"
SQ   SEQUENCE   592 AA;  64858 MW;  B35D2F832F865198 CRC64;
     MSKIHTDLKK IDPITVQVVL GSLENVAVEM GHKLARMSYS SIIRESEDFG CALVDVRGQQ
     LCESSHSTPL QSGPIPGYIK GIREIMEDRN DTFNQGDVIM HNSPYHGASH GPDVGFCIPV
     FYKDELIGFS VTTAHHLDIG SSTPGSCGIV DAVDAYAEGL QFKAIKVYDQ GVKNRYVWDI
     LKDNIRAPKL VVGDMEAQIA AARIGAQRYI EIIEKYGLDT VQAASEELMN YSEKMMRDAI
     KKLPDGEYTA EGFLDGYLDS DDPAKKDLRI NVTVKVDGSD LTVDLTGTSP QVTDKPINMP
     LLGTVDIAIY LTLRSILLDS TVYGNFPQNS GLIRPIKIVA PKGTLCNPIF PAPTIARFNS
     GNAVADTLMK ALAQVVPHQV SAGVGNLQVV AFSGQSNENY WVYMDIMEGS YGGRYGKDGM
     DAVDTLYANT RNNPIEDIES HYPLRVNRYE LRDNDSAPGK WRGGIGSIRE VSFLADGSFS
     VEADGHKYAP WGFDDGQDGY VGSLSIRDNE TNELVQLPSK LPNRHAQSGS TIQLVGPCGG
     GYGNPLEREP EKVLSDYLDG FITKEKALVE YGVTITDSEE IDYEKTNELR KV