I20L2_HUMAN
ID I20L2_HUMAN Reviewed; 353 AA.
AC Q9H9L3; D3DVC6; Q64KA2;
DT 16-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Interferon-stimulated 20 kDa exonuclease-like 2;
DE EC=3.1.-.-;
GN Name=ISG20L2; ORFNames=HSD-38, HSD38;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RA Yang C.B., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.;
RT "A new spermatogenesis-related gene.";
RL Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=12429849; DOI=10.1091/mbc.e02-05-0271;
RA Scherl A., Coute Y., Deon C., Calle A., Kindbeiter K., Sanchez J.-C.,
RA Greco A., Hochstrasser D.F., Diaz J.-J.;
RT "Functional proteomic analysis of human nucleolus.";
RL Mol. Biol. Cell 13:4100-4109(2002).
RN [7]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RX PubMed=18065403; DOI=10.1074/mcp.m700510-mcp200;
RA Coute Y., Kindbeiter K., Belin S., Dieckmann R., Duret L., Bezin L.,
RA Sanchez J.-C., Diaz J.-J.;
RT "ISG20L2, a novel vertebrate nucleolar exoribonuclease involved in ribosome
RT biogenesis.";
RL Mol. Cell. Proteomics 7:546-559(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: 3'-> 5'-exoribonuclease involved in ribosome biogenesis in
CC the processing of the 12S pre-rRNA. Displays a strong specificity for a
CC 3'-end containing a free hydroxyl group. {ECO:0000269|PubMed:18065403}.
CC -!- INTERACTION:
CC Q9H9L3; O00423: EML1; NbExp=3; IntAct=EBI-751335, EBI-751327;
CC Q9H9L3; P16333: NCK1; NbExp=2; IntAct=EBI-751335, EBI-389883;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:12429849,
CC ECO:0000269|PubMed:18065403}.
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DR EMBL; AY391837; AAS55433.1; -; mRNA.
DR EMBL; AK022733; BAB14212.1; -; mRNA.
DR EMBL; AL590666; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471121; EAW52919.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW52920.1; -; Genomic_DNA.
DR EMBL; BC000575; AAH00575.1; -; mRNA.
DR CCDS; CCDS1153.1; -.
DR RefSeq; NP_001290024.1; NM_001303095.1.
DR RefSeq; XP_005245573.1; XM_005245516.4.
DR RefSeq; XP_006711623.1; XM_006711560.3.
DR RefSeq; XP_006711624.1; XM_006711561.3.
DR AlphaFoldDB; Q9H9L3; -.
DR SMR; Q9H9L3; -.
DR BioGRID; 123618; 35.
DR IntAct; Q9H9L3; 22.
DR MINT; Q9H9L3; -.
DR STRING; 9606.ENSP00000323424; -.
DR iPTMnet; Q9H9L3; -.
DR PhosphoSitePlus; Q9H9L3; -.
DR BioMuta; ISG20L2; -.
DR DMDM; 42559527; -.
DR SWISS-2DPAGE; Q9H9L3; -.
DR EPD; Q9H9L3; -.
DR jPOST; Q9H9L3; -.
DR MassIVE; Q9H9L3; -.
DR MaxQB; Q9H9L3; -.
DR PaxDb; Q9H9L3; -.
DR PeptideAtlas; Q9H9L3; -.
DR PRIDE; Q9H9L3; -.
DR ProteomicsDB; 81328; -.
DR Antibodypedia; 20441; 43 antibodies from 20 providers.
DR DNASU; 81875; -.
DR Ensembl; ENST00000313146.10; ENSP00000323424.6; ENSG00000143319.17.
DR Ensembl; ENST00000368219.2; ENSP00000357202.2; ENSG00000143319.17.
DR GeneID; 81875; -.
DR KEGG; hsa:81875; -.
DR MANE-Select; ENST00000368219.2; ENSP00000357202.2; NM_001370150.2; NP_001357079.1.
DR UCSC; uc001fps.2; human.
DR CTD; 81875; -.
DR GeneCards; ISG20L2; -.
DR HGNC; HGNC:25745; ISG20L2.
DR HPA; ENSG00000143319; Tissue enriched (testis).
DR MIM; 611930; gene.
DR neXtProt; NX_Q9H9L3; -.
DR OpenTargets; ENSG00000143319; -.
DR PharmGKB; PA142671650; -.
DR VEuPathDB; HostDB:ENSG00000143319; -.
DR eggNOG; KOG2249; Eukaryota.
DR GeneTree; ENSGT00940000159724; -.
DR HOGENOM; CLU_022453_0_0_1; -.
DR InParanoid; Q9H9L3; -.
DR OMA; PCPIVDY; -.
DR OrthoDB; 1562214at2759; -.
DR PhylomeDB; Q9H9L3; -.
DR TreeFam; TF354340; -.
DR PathwayCommons; Q9H9L3; -.
DR Reactome; R-HSA-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR SignaLink; Q9H9L3; -.
DR BioGRID-ORCS; 81875; 677 hits in 1076 CRISPR screens.
DR ChiTaRS; ISG20L2; human.
DR GenomeRNAi; 81875; -.
DR Pharos; Q9H9L3; Tdark.
DR PRO; PR:Q9H9L3; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; Q9H9L3; protein.
DR Bgee; ENSG00000143319; Expressed in right testis and 197 other tissues.
DR Genevisible; Q9H9L3; HS.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000175; F:3'-5'-exoribonuclease activity; IEA:InterPro.
DR GO; GO:0004527; F:exonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0042254; P:ribosome biogenesis; IEA:UniProtKB-KW.
DR CDD; cd06149; ISG20; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR037433; ISG20_DEDDh.
DR InterPro; IPR034933; ISG20L2.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR12801:SF78; PTHR12801:SF78; 1.
DR Pfam; PF00929; RNase_T; 1.
DR SMART; SM00479; EXOIII; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW Exonuclease; Hydrolase; Nuclease; Nucleus; Reference proteome;
KW Ribosome biogenesis.
FT CHAIN 1..353
FT /note="Interferon-stimulated 20 kDa exonuclease-like 2"
FT /id="PRO_0000084121"
FT DOMAIN 178..353
FT /note="Exonuclease"
FT REGION 1..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..92
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 151..171
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 130
FT /note="N -> S (in dbSNP:rs3795737)"
FT /id="VAR_038171"
SQ SEQUENCE 353 AA; 39154 MW; 07558CDED402C195 CRC64;
MSTLLLNLDF GEPPPKKALE GNAKHRNFVK KRRLLERRGF LSKKNQPPSK APKLHSEPSK
KGETPTVDGT WKTPSFPKKK TAASSNGSGQ PLDKKAAVSW LTPAPSKKAD SVAAKVDLLG
EFQSALPKIN SHPTRSQKKS SQKKSSKKNH PQKNAPQNST QAHSENKCSG ASQKLPRKMV
AIDCEMVGTG PKGHVSSLAR CSIVNYNGDV LYDEYILPPC HIVDYRTRWS GIRKQHMVNA
TPFKIARGQI LKILTGKIVV GHAIHNDFKA LQYFHPKSLT RDTSHIPPLN RKADCPENAT
MSLKHLTKKL LNRDIQVGKS GHSSVEDAQA TMELYKLVEV EWEEHLARNP PTD
