I27RA_MOUSE
ID I27RA_MOUSE Reviewed; 623 AA.
AC O70394; E9QNY4;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Interleukin-27 receptor subunit alpha;
DE Short=IL-27 receptor subunit alpha;
DE Short=IL-27R subunit alpha;
DE Short=IL-27R-alpha;
DE Short=IL-27RA;
DE AltName: Full=Type I T-cell cytokine receptor;
DE Short=TCCR;
DE AltName: Full=WSX-1;
DE Flags: Precursor;
GN Name=Il27ra; Synonyms=Tccr, Wsx1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Spleen;
RX PubMed=9600072; DOI=10.1006/bbrc.1998.8576;
RA Sprecher C.A., Grant F.J., Baumgartner J.W., Presnell S.R., Schrader S.K.,
RA Yamagiwa T., Whitmore T.E., O'Hara P.J., Foster D.F.;
RT "Cloning and characterization of a novel class I cytokine receptor.";
RL Biochem. Biophys. Res. Commun. 246:82-90(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Spleen;
RX PubMed=11057672; DOI=10.1038/35038103;
RA Chen Q., Ghilardi N., Wang H., Baker T., Xie M.-H., Gurney A., Grewal I.S.,
RA de Sauvage F.J.;
RT "Development of Th1-type immune responses requires the type I cytokine
RT receptor TCCR.";
RL Nature 407:916-920(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Receptor for IL27. Requires IL6ST/GP130 to mediate signal
CC transduction in response to IL27. This signaling system acts through
CC STAT3 and STAT1. Involved in the regulation of Th1-type immune
CC responses. Also appears to be involved in innate defense mechanisms.
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in CD4+ and CD8+ T-cells, B-cells,
CC natural killer cells and macrophages. Highest levels in CD4+ T-cells
CC and natural killer cells. Expression highest in Th0 cells.
CC {ECO:0000269|PubMed:11057672}.
CC -!- INDUCTION: Down-regulated on differentiation of CD4+ T-cells in both
CC Th1 and TH2 cells.
CC -!- DOMAIN: The WSXWS motif appears to be necessary for proper protein
CC folding and thereby efficient intracellular transport and cell-surface
CC receptor binding.
CC -!- DOMAIN: The box 1 motif is required for JAK interaction and/or
CC activation.
CC -!- SIMILARITY: Belongs to the type I cytokine receptor family. Type 2
CC subfamily. {ECO:0000305}.
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DR EMBL; AF053005; AAC40121.1; -; mRNA.
DR EMBL; AF265243; AAG27298.1; -; mRNA.
DR EMBL; AC156028; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC032878; AAH32878.1; -; mRNA.
DR CCDS; CCDS22465.1; -.
DR RefSeq; NP_057880.2; NM_016671.3.
DR AlphaFoldDB; O70394; -.
DR SMR; O70394; -.
DR STRING; 10090.ENSMUSP00000005601; -.
DR GlyGen; O70394; 6 sites.
DR PhosphoSitePlus; O70394; -.
DR SwissPalm; O70394; -.
DR EPD; O70394; -.
DR MaxQB; O70394; -.
DR PaxDb; O70394; -.
DR PRIDE; O70394; -.
DR ProteomicsDB; 267033; -.
DR Antibodypedia; 13590; 483 antibodies from 35 providers.
DR DNASU; 50931; -.
DR Ensembl; ENSMUST00000005601; ENSMUSP00000005601; ENSMUSG00000005465.
DR GeneID; 50931; -.
DR KEGG; mmu:50931; -.
DR UCSC; uc009mlr.2; mouse.
DR CTD; 9466; -.
DR MGI; MGI:1355318; Il27ra.
DR VEuPathDB; HostDB:ENSMUSG00000005465; -.
DR eggNOG; ENOG502RF72; Eukaryota.
DR GeneTree; ENSGT00700000104610; -.
DR HOGENOM; CLU_029896_0_0_1; -.
DR InParanoid; O70394; -.
DR OMA; TCCCSLI; -.
DR OrthoDB; 204535at2759; -.
DR PhylomeDB; O70394; -.
DR TreeFam; TF338122; -.
DR Reactome; R-MMU-8984722; Interleukin-35 Signalling.
DR Reactome; R-MMU-9020956; Interleukin-27 signaling.
DR BioGRID-ORCS; 50931; 3 hits in 109 CRISPR screens.
DR ChiTaRS; Il27ra; mouse.
DR PRO; PR:O70394; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; O70394; protein.
DR Bgee; ENSMUSG00000005465; Expressed in ileal epithelium and 63 other tissues.
DR Genevisible; O70394; MM.
DR GO; GO:0005737; C:cytoplasm; TAS:Reactome.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0042022; C:interleukin-12 receptor complex; IBA:GO_Central.
DR GO; GO:0072536; C:interleukin-23 receptor complex; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR GO; GO:0019955; F:cytokine binding; IBA:GO_Central.
DR GO; GO:0004896; F:cytokine receptor activity; IBA:GO_Central.
DR GO; GO:0045509; F:interleukin-27 receptor activity; IDA:MGI.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:MGI.
DR GO; GO:0002692; P:negative regulation of cellular extravasation; IGI:BHF-UCL.
DR GO; GO:0032700; P:negative regulation of interleukin-17 production; IGI:BHF-UCL.
DR GO; GO:0032715; P:negative regulation of interleukin-6 production; IGI:BHF-UCL.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISO:MGI.
DR GO; GO:2000408; P:negative regulation of T cell extravasation; IGI:BHF-UCL.
DR GO; GO:2000317; P:negative regulation of T-helper 17 type immune response; IGI:BHF-UCL.
DR GO; GO:0032720; P:negative regulation of tumor necrosis factor production; IGI:BHF-UCL.
DR GO; GO:0002829; P:negative regulation of type 2 immune response; IMP:MGI.
DR GO; GO:0042104; P:positive regulation of activated T cell proliferation; IBA:GO_Central.
DR GO; GO:0032729; P:positive regulation of interferon-gamma production; IMP:MGI.
DR GO; GO:0002827; P:positive regulation of T-helper 1 type immune response; IMP:MGI.
DR GO; GO:0048302; P:regulation of isotype switching to IgG isotypes; IMP:MGI.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR SMART; SM00060; FN3; 3.
DR SUPFAM; SSF49265; SSF49265; 2.
DR PROSITE; PS50853; FN3; 4.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Receptor; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..623
FT /note="Interleukin-27 receptor subunit alpha"
FT /id="PRO_0000010877"
FT TOPO_DOM 25..510
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 511..531
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 532..623
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 30..124
FT /note="Fibronectin type-III 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 125..225
FT /note="Fibronectin type-III 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 316..412
FT /note="Fibronectin type-III 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 413..505
FT /note="Fibronectin type-III 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT MOTIF 211..215
FT /note="WSXWS motif"
FT MOTIF 552..560
FT /note="Box 1 motif"
FT CARBOHYD 46
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 296
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 305
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 360
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 461
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 156..158
FT /note="VLI -> ALT (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT AAH32878)"
FT /evidence="ECO:0000305"
FT CONFLICT 170
FT /note="T -> A (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT AAH32878)"
FT /evidence="ECO:0000305"
FT CONFLICT 476
FT /note="L -> S (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT AAH32878)"
FT /evidence="ECO:0000305"
FT CONFLICT 497
FT /note="N -> D (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT AAH32878)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 623 AA; 69093 MW; D683D282480242F0 CRC64;
MNRLRVARLT PLELLLSLMS LLLGTRPHGS PGPLQCYSVG PLGILNCSWE PLGDLETPPV
LYHQSQKYHP NRVWEVKVPS KQSWVTIPRE QFTMADKLLI WGTQKGRPLW SSVSVNLETQ
MKPDTPQIFS QVDISEEATL EATVQWAPPV WPPQKVLICQ FRYKECQAET WTRLEPQLKT
DGLTPVEMQN LEPGTCYQVS GRCQVENGYP WGEWSSPLSF QTPFLDPEDV WVSGTVCETS
GKRAALLVWK DPRPCVQVTY TVWFGAGDIT TTQEEVPCCK SPVPAWMEWA VVSPGNSTSW
VPPTNLSLVC LAPESAPCDV GVSSADGSPG IKVTWKQGTR KPLEYVVDWA QDGDSLDKLN
WTRLPPGNLS TLLPGEFKGG VPYRITVTAV YSGGLAAAPS VWGFREELVP LAGPAVWRLP
DDPPGTPVVA WGEVPRHQLR GQATHYTFCI QSRGLSTVCR NVSSQTQTAT LPNLHLGSFK
LWVTVSTVAG QGPPGPNLSL HLPDNRIRWK ALPWFLSLWG LLLMGCGLSL ASTRCLQARC
LHWRHKLLPQ WIWERVPDPA NSNSGQPYIK EVSLPQPPKD GPILEVEEVE LQPVVESPKA
SAPIYSGYEK HFLPTPEELG LLV
