ID   APAM_APICC              Reviewed;          46 AA.
AC   Q86QT2;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Apamin {ECO:0000312|EMBL:AAO19578.1};
DE   Flags: Precursor;
OS   Apis cerana cerana (Oriental honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=94128;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Zhang S.F., Shi W.J., Cheng J.A., Zhang C.X.;
RT   "Cloning and comparison of the genes encoding precursor of apamin from the
RT   venom of honeybees and wasps.";
RL   Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Neurotoxin that blocks voltage-independent calcium-activated
CC       potassium channels (KCNN1=SK1, KCNN2=SK2, KCNN3=SK3).
CC       {ECO:0000250|UniProtKB:P01500}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P01500}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
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DR   EMBL; AY164489; AAO19578.1; -; mRNA.
DR   AlphaFoldDB; Q86QT2; -.
DR   BMRB; Q86QT2; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR035361; Bee_toxin.
DR   Pfam; PF17454; Bee_toxin; 1.
PE   3: Inferred from homology;
KW   Amidation; Calcium-activated potassium channel impairing toxin;
KW   Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   PEPTIDE         28..45
FT                   /note="Apamin"
FT                   /id="PRO_0000247528"
FT   REGION          40..41
FT                   /note="Essential for toxin activity"
FT                   /evidence="ECO:0000250|UniProtKB:P01500"
FT   MOD_RES         45
FT                   /note="Histidine amide"
FT                   /evidence="ECO:0000250|UniProtKB:P01500"
FT   DISULFID        28..38
FT                   /evidence="ECO:0000250|UniProtKB:P01500"
FT   DISULFID        30..42
FT                   /evidence="ECO:0000250|UniProtKB:P01500"
SQ   SEQUENCE   46 AA;  5107 MW;  10694A107CD0E0D3 CRC64;
     MISMLRCISL FLSVILITGY FVTPVMSCNC KAPETALCAR RCQQHG