ID   APA_MYCBO               Reviewed;         325 AA.
AC   O30620; A0A1R3Y0C8; X2BJF2;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Alanine and proline-rich secreted protein Apa;
DE   AltName: Full=45/47 kDa antigen;
DE   AltName: Full=FAP-B;
DE   AltName: Full=Fibronectin attachment protein;
DE   Flags: Precursor;
GN   Name=apa; OrderedLocusNames=BQ2027_MB1891;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BCG;
RA   Zhao W., Schorey J.S., Bong-Mastek M., Brown E.J., Ratliff T.L.;
RT   "Identification, sequence and characterization of the M. bovis BCG
RT   fibronectin attachment protein.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the Apa family. {ECO:0000305}.
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DR   EMBL; AF013569; AAB71842.1; -; Genomic_DNA.
DR   EMBL; LT708304; SIU00495.1; -; Genomic_DNA.
DR   RefSeq; NP_855543.1; NC_002945.3.
DR   RefSeq; WP_003409337.1; NC_002945.4.
DR   AlphaFoldDB; O30620; -.
DR   SMR; O30620; -.
DR   EnsemblBacteria; SIU00495; SIU00495; BQ2027_MB1891.
DR   GeneID; 45425833; -.
DR   PATRIC; fig|233413.5.peg.2072; -.
DR   OMA; WVESDAS; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050840; F:extracellular matrix binding; IEA:InterPro.
DR   InterPro; IPR010801; FAP.
DR   Pfam; PF07174; FAP; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Repeat; Secreted; Signal.
FT   SIGNAL          1..39
FT                   /evidence="ECO:0000250"
FT   CHAIN           40..325
FT                   /note="Alanine and proline-rich secreted protein Apa"
FT                   /id="PRO_0000020743"
FT   REPEAT          85..88
FT                   /note="1"
FT   REPEAT          94..97
FT                   /note="2"
FT   REPEAT          104..107
FT                   /note="3"
FT   REGION          38..110
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          85..107
FT                   /note="3 X 4 AA approximate repeats of [DA]-P-N-A"
FT   REGION          286..325
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        39..107
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        49
FT                   /note="O-linked (Man...) threonine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        57
FT                   /note="O-linked (Man...) threonine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        66
FT                   /note="O-linked (Man) threonine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        316
FT                   /note="O-linked (Man...) threonine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   325 AA;  32687 MW;  D3419CA5547D91E9 CRC64;
     MHQVDPNLTR RKGRLAALAI AAMASASLVT VAVPATANAD PEPAPPVPTT AASPPSTAAA
     PPAPATPVAP PPPAAANTPN AQPGDPNAAP PPADPNAPPP PVIAPNAPQP VRIDNPVGGF
     SFALPAGWVE SDAAHLDYGS ALLSKTTGDP PFPGQPPPVA NDTRIVLGRL DQKLYASAEA
     TDSKAAARLG SDMGEFYMPY PGTRINQETV SLDANGVSGS ASYYEVKFSD PSKPNGQIWT
     GVIGSPAANA PDAGPPQRWF VVWLGTANNP VDKGAAKALA ESIRPLVAPP PAPAPAPAEP
     APAPAPAGEV APTPTTPTPQ RTLPA