ID   I5_VACCW                Reviewed;          79 AA.
AC   P12924; Q76ZU8;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   02-JUN-2021, entry version 62.
DE   RecName: Full=Protein I5;
DE   AltName: Full=Protein VP13K;
GN   OrderedLocusNames=VACWR074; ORFNames=I5L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2835495; DOI=10.1128/jvi.62.6.1889-1897.1988;
RA   Schmitt J.F.C., Stunnenberg H.G.;
RT   "Sequence and transcriptional analysis of the vaccinia virus HindIII I
RT   fragment.";
RL   J. Virol. 62:1889-1897(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 1-11.
RC   STRAIN=IHD-J;
RX   PubMed=8030247; DOI=10.1006/viro.1994.1406;
RA   Takahashi T., Oie M., Ichihashi Y.;
RT   "N-terminal amino acid sequences of vaccinia virus structural proteins.";
RL   Virology 202:844-852(1994).
RN   [4]
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=18701595; DOI=10.1128/jvi.01355-08;
RA   Sood C.L., Ward J.M., Moss B.;
RT   "Vaccinia virus encodes I5, a small hydrophobic virion membrane protein
RT   that enhances replication and virulence in mice.";
RL   J. Virol. 82:10071-10078(2008).
RN   [5]
RP   SUBCELLULAR LOCATION, AND TOPOLOGY.
RX   PubMed=19077320; DOI=10.1186/1743-422x-5-148;
RA   Unger B., Nichols R.J., Stanitsa E.S., Traktman P.;
RT   "Functional characterization of the vaccinia virus I5 protein.";
RL   Virol. J. 5:148-148(2008).
CC   -!- FUNCTION: Envelope protein.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000269|PubMed:18701595,
CC       ECO:0000269|PubMed:19077320}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:18701595, ECO:0000269|PubMed:19077320}.
CC       Note=Probably localizes to the membrane of mature virions (MV).
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:18701595}.
CC   -!- SIMILARITY: Belongs to the Chordopoxvirinae I5 family. {ECO:0000305}.
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DR   EMBL; J03399; AAB59807.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89353.1; -; Genomic_DNA.
DR   PIR; E29889; WZVZI5.
DR   RefSeq; YP_232956.1; NC_006998.1.
DR   DNASU; 3707607; -.
DR   GeneID; 3707607; -.
DR   KEGG; vg:3707607; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006803; Poxvirus_I5.
DR   Pfam; PF04713; Pox_I5; 1.
DR   PIRSF; PIRSF003768; VAC_I5L; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Late protein; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT   INIT_MET        1
FT                   /note="Removed; partial"
FT   CHAIN           2..79
FT                   /note="Protein I5"
FT                   /id="PRO_0000099576"
FT   TOPO_DOM        2..8
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..47
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        69..79
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   79 AA;  8744 MW;  37F4826B71CF04C3 CRC64;
     MVDAITVLTA IGITVLMLLM VISGAALIVK ELNPNDIFTM QSLKFNRAVT IFKYIGLFIY
     IPGTIILYAT YVKSLLMKS