ID   I6_VACCW                Reviewed;         382 AA.
AC   P68462; P12925; Q76ZU7;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   23-FEB-2022, entry version 43.
DE   RecName: Full=Telomere-binding protein I6;
GN   OrderedLocusNames=VACWR075; ORFNames=I6L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2835495; DOI=10.1128/jvi.62.6.1889-1897.1988;
RA   Schmitt J.F.C., Stunnenberg H.G.;
RT   "Sequence and transcriptional analysis of the vaccinia virus HindIII I
RT   fragment.";
RL   J. Virol. 62:1889-1897(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 115-124; 262-274 AND 276-289, AND FUNCTION.
RX   PubMed=11581377; DOI=10.1128/jvi.75.21.10090-10105.2001;
RA   DeMasi J., Du S., Lennon D., Traktman P.;
RT   "Vaccinia virus telomeres: interaction with the viral I1, I6, and K4
RT   proteins.";
RL   J. Virol. 75:10090-10105(2001).
RN   [4]
RP   FUNCTION, AND MUTAGENESIS OF 14-LYS--LYS-18; 56-GLU--LYS-60;
RP   103-ASP--ASP-105; 145-GLU--GLU-147; 196-LYS--ARG-198; 305-LYS-ARG-306;
RP   359-LYS--LYS-361 AND 364-LYS-LYS-365.
RX   PubMed=14512543; DOI=10.1128/jvi.77.20.10929-10942.2003;
RA   Grubisha O., Traktman P.;
RT   "Genetic analysis of the vaccinia virus I6 telomere-binding protein
RT   uncovers a key role in genome encapsidation.";
RL   J. Virol. 77:10929-10942(2003).
CC   -!- FUNCTION: Binds to the hairpin form of the viral telomeric sequence.
CC       Might direct genome encapsidation into the virus particle.
CC       {ECO:0000269|PubMed:11581377, ECO:0000269|PubMed:14512543}.
CC   -!- SUBCELLULAR LOCATION: Virion. Note=Present in the virus core.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae I6 family. {ECO:0000305}.
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DR   EMBL; J03399; AAB59808.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89354.1; -; Genomic_DNA.
DR   PIR; F29889; WZVZI6.
DR   RefSeq; YP_232957.1; NC_006998.1.
DR   DNASU; 3707608; -.
DR   GeneID; 3707608; -.
DR   KEGG; vg:3707608; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR007674; Poxvirus_36kDa_major_membrane.
DR   InterPro; IPR022219; Poxvirus_I6_C.
DR   Pfam; PF12562; DUF3746; 1.
DR   Pfam; PF04595; Pox_I6; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; DNA-binding; Reference proteome; Virion.
FT   CHAIN           1..382
FT                   /note="Telomere-binding protein I6"
FT                   /id="PRO_0000099579"
FT   MUTAGEN         14..18
FT                   /note="KPTKK->APTAA: Reduced plaque size at 32 degrees
FT                   Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
FT   MUTAGEN         56..60
FT                   /note="EDVLK->AAVLA: Reduced plaque size at 32 degrees
FT                   Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
FT   MUTAGEN         103..105
FT                   /note="DDD->AAA: No change in amounts of infectious virus
FT                   produced during a single round of infection at 32 degrees
FT                   Celsius or 40 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
FT   MUTAGEN         145..147
FT                   /note="EKE->AAA: No change in amounts of infectious virus
FT                   produced during a single round of infection at 32 degrees
FT                   Celsius or 40 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
FT   MUTAGEN         196..198
FT                   /note="KER->AAA: No change in amounts of infectious virus
FT                   produced during a single round of infection at 32 degrees
FT                   Celsius or 40 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
FT   MUTAGEN         305..306
FT                   /note="KR->AA: No change in amounts of infectious virus
FT                   produced during a single round of infection at 32 degrees
FT                   Celsius or 40 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
FT   MUTAGEN         359..361
FT                   /note="KKK->AAA: 100-fold reduction in viral yield at 40
FT                   degrees Celsius. Failure in genome encapsidation at 40
FT                   degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
FT   MUTAGEN         364..365
FT                   /note="KK->AA: Reduced plaque size at 32 degrees Celsius."
FT                   /evidence="ECO:0000269|PubMed:14512543"
SQ   SEQUENCE   382 AA;  43454 MW;  4D0F72666DEE1307 CRC64;
     MNNFVKQVAS KSLKPTKKLS PSDEVISLNE CIISFNLDNF YYCNDGLFTK PINTPEDVLK
     SLLIMESFAY EKMIIKGLIK ILISRAYIND IYFTPFGWLT GVDDDPETHV VIKIIFNSSL
     ISIKSQVIEY LKPYNVNNLS VLTTEKELSI NTFNVPDSIP MSIISFFPFD TDFILVILFF
     GVYNDSYCGI SYISPKERLP YIIEILKPLV SEINMLSDEI GRTSSIRIFN STSVKKFPTN
     TLTSICEIVY SFDESSFPTP KTFTPLNASP YIPKKIVSLL DLPSNVEIKA ISRGGVDFIT
     HINNKRLNTI LVIAKDNFLK NSTFSGTFIK ENIIWKGIYT YRIIKSSFPV PTIKSVTNKK
     KICKKHCFVN SQYTTRTLSH IL