I7_VACCW
ID I7_VACCW Reviewed; 423 AA.
AC P12926; Q6RHZ8; Q76ZU6;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Core protease I7;
DE EC=3.4.22.-;
GN OrderedLocusNames=VACWR076; ORFNames=I7L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RX PubMed=2835495; DOI=10.1128/jvi.62.6.1889-1897.1988;
RA Schmitt J.F.C., Stunnenberg H.G.;
RT "Sequence and transcriptional analysis of the vaccinia virus HindIII I
RT fragment.";
RL J. Virol. 62:1889-1897(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-215.
RX PubMed=1994576; DOI=10.1016/0042-6822(91)90491-s;
RA Fathi Z., Condit R.C.;
RT "Genetic and molecular biological characterization of a vaccinia virus
RT temperature-sensitive complementation group affecting a virion component.";
RL Virology 181:258-272(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Rozanov D.V., Strongin A.Y.;
RT "Vaccinia virus strain WR cysteine proteinase I7L.";
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND ACTIVE SITES.
RX PubMed=12163618; DOI=10.1128/jvi.76.17.8973-8976.2002;
RA Byrd C.M., Bolken T.C., Hruby D.E.;
RT "The vaccinia virus I7L gene product is the core protein proteinase.";
RL J. Virol. 76:8973-8976(2002).
RN [6]
RP CHARACTERIZATION, AND MUTAGENESIS OF HIS-241; TRP-242; ASP-248; ASP-258;
RP GLN-322; CYS-328 AND GLY-329.
RX PubMed=14512576; DOI=10.1128/jvi.77.20.11279-11283.2003;
RA Byrd C.M., Bolken T.C., Hruby D.E.;
RT "Molecular dissection of the vaccinia virus I7L core protein proteinase.";
RL J. Virol. 77:11279-11283(2003).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF HIS-241 AND CYS-328.
RX PubMed=15163727; DOI=10.1128/jvi.78.12.6335-6343.2004;
RA Ansarah-Sobrinho C., Moss B.;
RT "Role of the I7 protein in proteolytic processing of vaccinia virus
RT membrane and core components.";
RL J. Virol. 78:6335-6343(2004).
CC -!- FUNCTION: Late protein responsible for processing most or all of the
CC viral core and membrane proteins known to undergo morphogenesis-
CC associated proteolysis. These proteolytic events are involved in the
CC transformation of immature virions (IV) into mature virions (MV).
CC Probably cleaves at least the A3, A10, L4, and A17 precursors
CC preferentially at Ala-Gly-|-Ala motifs. Also seems to process Ala-
CC Gly-|-Ser and Ala-Gly-|-Thr motifs (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:12163618, ECO:0000269|PubMed:15163727}.
CC -!- SUBCELLULAR LOCATION: Virion. Note=Present in the virion core.
CC -!- INDUCTION: Expressed late in the viral replicative cycle.
CC {ECO:0000269|PubMed:2835495}.
CC -!- SIMILARITY: Belongs to the peptidase C57 family. {ECO:0000305}.
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DR EMBL; J03399; AAB59809.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89355.1; -; Genomic_DNA.
DR EMBL; AY497361; AAR88657.1; -; Genomic_DNA.
DR PIR; G29889; WZVZI7.
DR RefSeq; YP_232958.1; NC_006998.1.
DR MEROPS; C57.001; -.
DR DNASU; 3707609; -.
DR GeneID; 3707609; -.
DR KEGG; vg:3707609; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR004970; Peptidase_C57.
DR Pfam; PF03290; Peptidase_C57; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Late protein; Protease; Reference proteome; Thiol protease;
KW Virion.
FT CHAIN 1..423
FT /note="Core protease I7"
FT /id="PRO_0000099583"
FT ACT_SITE 241
FT /evidence="ECO:0000269|PubMed:12163618"
FT ACT_SITE 248
FT /evidence="ECO:0000269|PubMed:12163618"
FT ACT_SITE 328
FT /evidence="ECO:0000269|PubMed:12163618"
FT MUTAGEN 241
FT /note="H->A: Loss of cleavage of A10, A3, A17 and L4."
FT /evidence="ECO:0000269|PubMed:14512576,
FT ECO:0000269|PubMed:15163727"
FT MUTAGEN 242
FT /note="W->A: Loss of cleavage of A10, A3, and L4."
FT /evidence="ECO:0000269|PubMed:14512576"
FT MUTAGEN 248
FT /note="D->A: Loss of cleavage of A10, A3, and L4."
FT /evidence="ECO:0000269|PubMed:14512576"
FT MUTAGEN 258
FT /note="D->A: No effect on cleavage of A3L, and L4. Loss of
FT cleavage of A10L."
FT /evidence="ECO:0000269|PubMed:14512576"
FT MUTAGEN 322
FT /note="Q->A: Loss of cleavage of A10, A3, and L4."
FT /evidence="ECO:0000269|PubMed:14512576"
FT MUTAGEN 328
FT /note="C->A: Loss of cleavage of A10, A3, A17 and L4."
FT /evidence="ECO:0000269|PubMed:14512576,
FT ECO:0000269|PubMed:15163727"
FT MUTAGEN 329
FT /note="G->A: Loss of cleavage of A10, A3, and L4."
FT /evidence="ECO:0000269|PubMed:14512576"
FT CONFLICT 420
FT /note="N -> D (in Ref. 4; AAR88657)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 423 AA; 49011 MW; 6EB5BF25D7FD9FEE CRC64;
MERYTDLVIS KIPELGFTNL LCHIYSLAGL CSNIDVSKFL TNCNGYVVEK YDKSTTAGKV
SCIPIGMMLE LVESGHLSRP NSSDELDQKK ELTDELKTRY HSIYDVFELP TSIPLAYFFK
PRLREKVSKA IDFSQMDLKI DDLSRKGIHT GENPKVVKMK IEPERGAWMS NRSIKNLVSQ
FAYGSEVDYI GQFDMRFLNS LAIHEKFDAF MNKHILSYIL KDKIKSSTSR FVMFGFCYLS
HWKCVIYDKK QCLVSFYDSG GNIPTEFHHY NNFYFYSFSD GFNTNHKHSV LDNTNCDIDV
LFRFFECTFG AKIGCINVEV NQLLESECGM FISLFMILCT RTPPKSFKSL KKVYTFFKFL
ADKKMTLFKS ILFNLHDLSL DITETDNAGL KEYKRMEKWT KKSINVICDK LTTKLNRIVN
DDE
