IAA1_HORVU
ID IAA1_HORVU Reviewed; 146 AA.
AC P16968; Q43483;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Alpha-amylase inhibitor BMAI-1;
DE AltName: Full=Alpha-amylase flour inhibitor;
DE AltName: Allergen=Hor v 1;
DE Flags: Precursor; Fragment;
GN Name=IAM1;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Abyssinian 2231; TISSUE=Endosperm;
RX PubMed=1421148; DOI=10.1007/bf00040604;
RA Mena M., Sanchez-Monge R., Gomez L., Salcedo G., Carbonero P.;
RT "A major barley allergen associated with baker's asthma disease is a
RT glycosylated monomeric inhibitor of insect alpha-amylase: cDNA cloning and
RT chromosomal location of the gene.";
RL Plant Mol. Biol. 20:451-458(1992).
RN [2]
RP PROTEIN SEQUENCE OF 15-34.
RC STRAIN=cv. Bomi;
RX PubMed=2785932; DOI=10.1016/0014-5793(89)80444-2;
RA Barber D., Sanchez-Monge R., Gomez L., Carpizo J., Armentia A.,
RA Lopez-Otin C., Juan F., Salcedo G.;
RT "A barley flour inhibitor of insect alpha-amylase is a major allergen
RT associated with baker's asthma disease.";
RL FEBS Lett. 248:119-122(1989).
CC -!- FUNCTION: Could be involved in insect defense mechanisms. Inhibits
CC insect-type alpha-amylase.
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Endosperm.
CC -!- PTM: Five disulfide bonds are present (Probable), which are essential
CC for the inhibitor activity.
CC -!- PTM: Glycosylated.
CC -!- ALLERGEN: Causes an allergic reaction in human. Involved in baker's
CC asthma.
CC -!- SIMILARITY: Belongs to the protease inhibitor I6 (cereal trypsin/alpha-
CC amylase inhibitor) family. {ECO:0000305}.
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DR EMBL; X63517; CAA45085.1; -; mRNA.
DR PIR; S26197; S26197.
DR AlphaFoldDB; P16968; -.
DR SMR; P16968; -.
DR Allergome; 3328; Hor v 15.0101.
DR Allergome; 418; Hor v 15.
DR EnsemblPlants; HORVU.MOREX.r2.2HG0176960.1; HORVU.MOREX.r2.2HG0176960.1.CDS.1; HORVU.MOREX.r2.2HG0176960.
DR EnsemblPlants; HORVU.MOREX.r2.2HG0176960.1.mrna1; HORVU.MOREX.r2.2HG0176960.1.mrna1.cds1; HORVU.MOREX.r2.2HG0176960.1.
DR Gramene; HORVU.MOREX.r2.2HG0176960.1; HORVU.MOREX.r2.2HG0176960.1.CDS.1; HORVU.MOREX.r2.2HG0176960.
DR Gramene; HORVU.MOREX.r2.2HG0176960.1.mrna1; HORVU.MOREX.r2.2HG0176960.1.mrna1.cds1; HORVU.MOREX.r2.2HG0176960.1.
DR ExpressionAtlas; P16968; baseline.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0015066; F:alpha-amylase inhibitor activity; IDA:CACAO.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:InterPro.
DR CDD; cd00261; AAI_SS; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR044723; AAI_SS_dom.
DR InterPro; IPR002411; Allergen/amylase_inhib_rice.
DR InterPro; IPR006106; Allergen/soft/tryp_amyl_inhib.
DR InterPro; IPR006105; Allergen/tryp_amyl_inhib_CS.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PIRSF; PIRSF001657; Allergen/amylase_inhib; 1.
DR PRINTS; PR00808; AMLASEINHBTR.
DR PRINTS; PR00809; RAGALLERGEN.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00426; CEREAL_TRYP_AMYL_INH; 1.
PE 1: Evidence at protein level;
KW Allergen; Alpha-amylase inhibitor; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Secreted; Signal.
FT SIGNAL <1..14
FT /evidence="ECO:0000269|PubMed:2785932"
FT CHAIN 15..146
FT /note="Alpha-amylase inhibitor BMAI-1"
FT /id="PRO_0000014355"
FT CARBOHYD 125
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT NON_TER 1
SQ SEQUENCE 146 AA; 15816 MW; 73F505186D1A48D8 CRC64;
PTSVAVDQGS MVSNSPGEWC WPGMGYPVYP FPRCRALVKS QCAGGQVVES IQKDCCRQIA
AIGDEWCICG ALGSMRGSMY KELGVALADD KATVAEVFPG CRTEVMDRAV ASLPAVCNQY
IPNTNGTDGV CYWLSYYQPP RQMSSR