ID   IAA1_STIHL              Reviewed;          44 AA.
AC   A0A0X1KGZ5;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   16-MAR-2016, sequence version 1.
DT   03-AUG-2022, entry version 13.
DE   RecName: Full=Alpha-amylase inhibitor helianthamide {ECO:0000303|PubMed:27066537};
OS   Stichodactyla helianthus (Sun anemone) (Stoichactis helianthus).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Stichodactylidae; Stichodactyla.
OX   NCBI_TaxID=6123;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, DISULFIDE BONDS, RECOMBINANT EXPRESSION, AND
RP   X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) IN COMPLEX WITH PORCINE PANCREATIC
RP   ALPHA-AMYLASE.
RX   PubMed=27066537; DOI=10.1021/acscentsci.5b00399;
RA   Tysoe C., Williams L.K., Keyzers R., Nguyen N.T., Tarling C., Wicki J.,
RA   Goddard-Borger E.D., Aguda A.H., Perry S., Foster L.J., Andersen R.J.,
RA   Brayer G.D., Withers S.G.;
RT   "Potent human alpha-amylase inhibition by the beta-defensin-like protein
RT   helianthamide.";
RL   ACS Cent. Sci. 2:154-161(2016).
CC   -!- FUNCTION: Specific pancreatic alpha-amylase (AMY2A) inhibitor. The
CC       recombinant peptide inhibits human pancreatic (Ki=0.01 nM) and porcine
CC       pancreatic alpha-amylases (Ki=0.1 nM). {ECO:0000269|PubMed:27066537}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:C0HK71}.
CC   -!- DOMAIN: Is structurally homologous to beta-defensins.
CC       {ECO:0000305|PubMed:27066537}.
CC   -!- PHARMACEUTICAL: Potential drug candidate for the treatment of the type
CC       2 diabetes mellitus. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sea anemone alpha-amylase inhibitor family.
CC       {ECO:0000305}.
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DR   PDB; 4X0N; X-ray; 2.60 A; B=1-44.
DR   PDBsum; 4X0N; -.
DR   SMR; A0A0X1KGZ5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Alpha-amylase inhibitor; Direct protein sequencing;
KW   Disulfide bond; Pharmaceutical; Secreted.
FT   CHAIN           1..44
FT                   /note="Alpha-amylase inhibitor helianthamide"
FT                   /evidence="ECO:0000269|PubMed:27066537"
FT                   /id="PRO_0000454102"
FT   REGION          7..10
FT                   /note="Inhibitory motif"
FT                   /evidence="ECO:0000269|PubMed:27066537"
FT   DISULFID        6..38
FT                   /evidence="ECO:0000269|PubMed:27066537,
FT                   ECO:0007744|PDB:4X0N"
FT   DISULFID        16..33
FT                   /evidence="ECO:0000269|PubMed:27066537,
FT                   ECO:0007744|PDB:4X0N"
FT   DISULFID        20..39
FT                   /evidence="ECO:0000269|PubMed:27066537,
FT                   ECO:0007744|PDB:4X0N"
FT   STRAND          6..9
FT                   /evidence="ECO:0007829|PDB:4X0N"
FT   STRAND          12..19
FT                   /evidence="ECO:0007829|PDB:4X0N"
FT   STRAND          25..28
FT                   /evidence="ECO:0007829|PDB:4X0N"
FT   HELIX           31..33
FT                   /evidence="ECO:0007829|PDB:4X0N"
FT   STRAND          36..40
FT                   /evidence="ECO:0007829|PDB:4X0N"
SQ   SEQUENCE   44 AA;  4690 MW;  47530BD01034114E CRC64;
     ESGNSCYIYH GVSGICKASC AEDEKAMAGM GVCEGHLCCY KTPW