IAA20_ARATH
ID IAA20_ARATH Reviewed; 175 AA.
AC O24410;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Auxin-responsive protein IAA20;
DE AltName: Full=Indoleacetic acid-induced protein 20;
GN Name=IAA20; OrderedLocusNames=At2g46990; ORFNames=F14M4.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-175.
RX PubMed=9342315; DOI=10.1073/pnas.94.22.11786;
RA Kim J., Harter K., Theologis A.;
RT "Protein-protein interactions among the Aux/IAA proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:11786-11791(1997).
RN [6]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036262; DOI=10.1023/a:1015255030047;
RA Liscum E., Reed J.W.;
RT "Genetics of Aux/IAA and ARF action in plant growth and development.";
RL Plant Mol. Biol. 49:387-400(2002).
RN [7]
RP TRANSCRIPTIONAL REPRESSION DOMAIN.
RX PubMed=14742873; DOI=10.1105/tpc.017384;
RA Tiwari S.B., Hagen G., Guilfoyle T.J.;
RT "Aux/IAA proteins contain a potent transcriptional repression domain.";
RL Plant Cell 16:533-543(2004).
CC -!- FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that
CC function as repressors of early auxin response genes at low auxin
CC concentrations. Repression is thought to result from the interaction
CC with auxin response factors (ARFs), proteins that bind to the auxin-
CC responsive promoter element (AuxRE). Formation of heterodimers with ARF
CC proteins may alter their ability to modulate early auxin response genes
CC expression. {ECO:0000269|PubMed:12036262}.
CC -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000250}.
CC -!- INTERACTION:
CC O24410; Q9FIW5: ANAC094; NbExp=3; IntAct=EBI-632272, EBI-25522986;
CC O24410; Q8L9Y3: ARR14; NbExp=3; IntAct=EBI-632272, EBI-1100737;
CC O24410; Q9ZUM0: At2g02160; NbExp=3; IntAct=EBI-632272, EBI-15206592;
CC O24410; Q9LS00: HHO1; NbExp=3; IntAct=EBI-632272, EBI-15203988;
CC O24410; P49677: IAA1; NbExp=6; IntAct=EBI-632272, EBI-630505;
CC O24410; Q38828: IAA10; NbExp=5; IntAct=EBI-632272, EBI-3946434;
CC O24410; Q38829: IAA11; NbExp=3; IntAct=EBI-632272, EBI-2367923;
CC O24410; Q38830: IAA12; NbExp=4; IntAct=EBI-632272, EBI-617608;
CC O24410; Q38831: IAA13; NbExp=5; IntAct=EBI-632272, EBI-1554143;
CC O24410; A0A2H1ZEF6: IAA15; NbExp=5; IntAct=EBI-632272, EBI-25524519;
CC O24410; O24407: IAA16; NbExp=5; IntAct=EBI-632272, EBI-632231;
CC O24410; P93830: IAA17; NbExp=6; IntAct=EBI-632272, EBI-632243;
CC O24410; O24408: IAA18; NbExp=4; IntAct=EBI-632272, EBI-2295525;
CC O24410; O24409: IAA19; NbExp=5; IntAct=EBI-632272, EBI-632257;
CC O24410; P49678: IAA2; NbExp=6; IntAct=EBI-632272, EBI-632343;
CC O24410; O24410: IAA20; NbExp=3; IntAct=EBI-632272, EBI-632272;
CC O24410; Q8LAL2: IAA26; NbExp=5; IntAct=EBI-632272, EBI-3947418;
CC O24410; Q9ZSY8: IAA27; NbExp=3; IntAct=EBI-632272, EBI-3946677;
CC O24410; Q9XFM0: IAA28; NbExp=6; IntAct=EBI-632272, EBI-3133404;
CC O24410; Q38822: IAA3; NbExp=6; IntAct=EBI-632272, EBI-307174;
CC O24410; Q8H174: IAA31; NbExp=5; IntAct=EBI-632272, EBI-3946408;
CC O24410; Q8RYC6: IAA32; NbExp=4; IntAct=EBI-632272, EBI-3946448;
CC O24410; Q9FKM7: IAA33; NbExp=6; IntAct=EBI-632272, EBI-3946739;
CC O24410; Q9C5X0: IAA34; NbExp=6; IntAct=EBI-632272, EBI-3946459;
CC O24410; P33077: IAA4; NbExp=6; IntAct=EBI-632272, EBI-632187;
CC O24410; P33078: IAA5; NbExp=4; IntAct=EBI-632272, EBI-3946487;
CC O24410; Q38824: IAA6; NbExp=5; IntAct=EBI-632272, EBI-1554124;
CC O24410; Q38825: IAA7; NbExp=4; IntAct=EBI-632272, EBI-602959;
CC O24410; Q38826: IAA8; NbExp=4; IntAct=EBI-632272, EBI-632200;
CC O24410; Q38827: IAA9; NbExp=3; IntAct=EBI-632272, EBI-632216;
CC O24410; Q9LSI4: MGH6.1; NbExp=3; IntAct=EBI-632272, EBI-15198743;
CC O24410; Q9LJW5: MIF2; NbExp=3; IntAct=EBI-632272, EBI-15191779;
CC O24410; Q9LTC4: MYB15; NbExp=3; IntAct=EBI-632272, EBI-21497119;
CC O24410; Q84JP1: NFYA7; NbExp=3; IntAct=EBI-632272, EBI-4444640;
CC O24410; Q9S7W5: TCP13; NbExp=3; IntAct=EBI-632272, EBI-4424877;
CC O24410; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-632272, EBI-4426144;
CC O24410; Q9M1U4: TCP16; NbExp=3; IntAct=EBI-632272, EBI-15198627;
CC O24410; Q9MAH8: TCP3; NbExp=3; IntAct=EBI-632272, EBI-25522447;
CC O24410; Q84MB2: TIFY8; NbExp=3; IntAct=EBI-632272, EBI-4426557;
CC O24410; Q9LVG2: TOE2; NbExp=3; IntAct=EBI-632272, EBI-4424568;
CC O24410; O04336: WRKY21; NbExp=3; IntAct=EBI-632272, EBI-1239118;
CC O24410; Q9LQW3: ZHD14; NbExp=3; IntAct=EBI-632272, EBI-1806701;
CC O24410; O64722: ZHD3; NbExp=3; IntAct=EBI-632272, EBI-1806244;
CC O24410; Q9SVL0: ZHD7; NbExp=3; IntAct=EBI-632272, EBI-1806382;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- INDUCTION: By auxin.
CC -!- DOMAIN: The N-terminal half of the protein contains two conserved
CC domains I and II. Domain I includes a slightly degenerated ERF-
CC associated amphiphilic repression (EAR) motif which seems to be
CC involved in the activity of transcriptional repression. Domain II is
CC required for the correct degradation of the protein through the SCF-
CC mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA
CC proteins and auxin response factors (ARFs) occur through their C-
CC terminal dimerization domains III and IV.
CC -!- SIMILARITY: Belongs to the Aux/IAA family. {ECO:0000305}.
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DR EMBL; AC004411; AAC34236.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10783.1; -; Genomic_DNA.
DR EMBL; AK117233; BAC41909.1; -; mRNA.
DR EMBL; AF348576; AAK15547.1; -; mRNA.
DR EMBL; U49076; AAB84357.1; -; mRNA.
DR PIR; T02188; T02188.
DR RefSeq; NP_182222.1; NM_130267.6.
DR AlphaFoldDB; O24410; -.
DR SMR; O24410; -.
DR BioGRID; 4648; 53.
DR IntAct; O24410; 51.
DR STRING; 3702.AT2G46990.1; -.
DR PaxDb; O24410; -.
DR PRIDE; O24410; -.
DR DNASU; 819313; -.
DR EnsemblPlants; AT2G46990.1; AT2G46990.1; AT2G46990.
DR GeneID; 819313; -.
DR Gramene; AT2G46990.1; AT2G46990.1; AT2G46990.
DR KEGG; ath:AT2G46990; -.
DR Araport; AT2G46990; -.
DR TAIR; locus:2041324; AT2G46990.
DR eggNOG; ENOG502S04C; Eukaryota.
DR HOGENOM; CLU_049393_3_1_1; -.
DR InParanoid; O24410; -.
DR OMA; SRANYHY; -.
DR OrthoDB; 1436796at2759; -.
DR PhylomeDB; O24410; -.
DR PRO; PR:O24410; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O24410; baseline and differential.
DR Genevisible; O24410; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010588; P:cotyledon vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0009630; P:gravitropism; IMP:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR GO; GO:0009647; P:skotomorphogenesis; IMP:TAIR.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003311; AUX_IAA.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31734; PTHR31734; 1.
DR Pfam; PF02309; AUX_IAA; 1.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..175
FT /note="Auxin-responsive protein IAA20"
FT /id="PRO_0000112851"
FT DOMAIN 84..173
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 34..38
FT /note="EAR-like (transcriptional repression)"
SQ SEQUENCE 175 AA; 19445 MW; A0DE41A5E4359F3B CRC64;
MGRGRSSSSS SIESSSKSNP FGASSSTRNL STDLRLGLSF GTSSGTQYFN GGYGYSVAAP
AVEDAEYVAA VEEEEENECN SVGSFYVKVN MEGVPIGRKI DLMSLNGYRD LIRTLDFMFN
ASILWAEEED MCNEKSHVLT YADKEGDWMM VGDVPWEMFL STVRRLKISR ANYHY
