IAA29_ARATH
ID IAA29_ARATH Reviewed; 251 AA.
AC Q93WC4; O49363;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Auxin-responsive protein IAA29;
DE AltName: Full=Indoleacetic acid-induced protein 29;
GN Name=IAA29; OrderedLocusNames=At4g32280; ORFNames=F10M6.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND FUNCTION.
RX PubMed=12036262; DOI=10.1023/a:1015255030047;
RA Liscum E., Reed J.W.;
RT "Genetics of Aux/IAA and ARF action in plant growth and development.";
RL Plant Mol. Biol. 49:387-400(2002).
RN [5]
RP TRANSCRIPTIONAL REPRESSION DOMAIN.
RX PubMed=14742873; DOI=10.1105/tpc.017384;
RA Tiwari S.B., Hagen G., Guilfoyle T.J.;
RT "Aux/IAA proteins contain a potent transcriptional repression domain.";
RL Plant Cell 16:533-543(2004).
CC -!- FUNCTION: Aux/IAA proteins are short-lived transcriptional factors that
CC function as repressors of early auxin response genes at low auxin
CC concentrations. Repression is thought to result from the interaction
CC with auxin response factors (ARFs), proteins that bind to the auxin-
CC responsive promoter element (AuxRE). Formation of heterodimers with ARF
CC proteins may alter their ability to modulate early auxin response genes
CC expression. {ECO:0000269|PubMed:12036262}.
CC -!- SUBUNIT: Homodimers and heterodimers. {ECO:0000250}.
CC -!- INTERACTION:
CC Q93WC4; Q8RYC8: ARF19; NbExp=3; IntAct=EBI-3946697, EBI-529887;
CC Q93WC4; Q94JM3: ARF2; NbExp=6; IntAct=EBI-3946697, EBI-1799262;
CC Q93WC4; Q9XFM0: IAA28; NbExp=5; IntAct=EBI-3946697, EBI-3133404;
CC Q93WC4; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-3946697, EBI-4426144;
CC Q93WC4; Q9LQW3: ZHD14; NbExp=3; IntAct=EBI-3946697, EBI-1806701;
CC Q93WC4; O64722: ZHD3; NbExp=3; IntAct=EBI-3946697, EBI-1806244;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- INDUCTION: By auxin. {ECO:0000250}.
CC -!- DOMAIN: The N-terminal half of the protein contains two conserved
CC domains I and II. Domain I includes a slightly degenerated ERF-
CC associated amphiphilic repression (EAR) motif which seems to be
CC involved in the activity of transcriptional repression. Domain II is
CC required for the correct degradation of the protein through the SCF-
CC mediated ubiquitin-proteasome pathway. Interactions between Aux/IAA
CC proteins and auxin response factors (ARFs) occur through their C-
CC terminal dimerization domains III and IV.
CC -!- SIMILARITY: Belongs to the Aux/IAA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK55727.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA16962.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g32280 has been split into 2 genes: At4g32280 and At4g32285.; Evidence={ECO:0000305};
CC Sequence=CAB79946.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g32280 has been split into 2 genes: At4g32280 and At4g32285.; Evidence={ECO:0000305};
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DR EMBL; AL021811; CAA16962.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161580; CAB79946.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86032.1; -; Genomic_DNA.
DR EMBL; AF380646; AAK55727.1; ALT_FRAME; mRNA.
DR EMBL; AY054137; AAL06798.1; -; mRNA.
DR PIR; T05400; T05400.
DR RefSeq; NP_567891.1; NM_119380.3.
DR AlphaFoldDB; Q93WC4; -.
DR SMR; Q93WC4; -.
DR BioGRID; 14647; 21.
DR IntAct; Q93WC4; 17.
DR STRING; 3702.AT4G32280.1; -.
DR iPTMnet; Q93WC4; -.
DR PaxDb; Q93WC4; -.
DR EnsemblPlants; AT4G32280.1; AT4G32280.1; AT4G32280.
DR GeneID; 829361; -.
DR Gramene; AT4G32280.1; AT4G32280.1; AT4G32280.
DR KEGG; ath:AT4G32280; -.
DR Araport; AT4G32280; -.
DR TAIR; locus:2116505; AT4G32280.
DR eggNOG; ENOG502S29N; Eukaryota.
DR HOGENOM; CLU_049393_4_0_1; -.
DR OMA; KSWRKKM; -.
DR OrthoDB; 1486853at2759; -.
DR PhylomeDB; Q93WC4; -.
DR PRO; PR:Q93WC4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93WC4; baseline and differential.
DR Genevisible; Q93WC4; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0009733; P:response to auxin; TAS:TAIR.
DR GO; GO:0010583; P:response to cyclopentenone; IEP:TAIR.
DR GO; GO:0010218; P:response to far red light; IEP:TAIR.
DR GO; GO:0009635; P:response to herbicide; IEP:TAIR.
DR GO; GO:0010114; P:response to red light; IEP:TAIR.
DR InterPro; IPR033389; AUX/IAA_dom.
DR InterPro; IPR003311; AUX_IAA.
DR InterPro; IPR000270; PB1_dom.
DR PANTHER; PTHR31734; PTHR31734; 1.
DR Pfam; PF02309; AUX_IAA; 2.
DR PROSITE; PS51745; PB1; 1.
PE 1: Evidence at protein level;
KW Auxin signaling pathway; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..251
FT /note="Auxin-responsive protein IAA29"
FT /id="PRO_0000112855"
FT DOMAIN 159..246
FT /note="PB1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01081"
FT MOTIF 3..7
FT /note="EAR-like (transcriptional repression)"
SQ SEQUENCE 251 AA; 28610 MW; 47A6ECBD4F0ECC3B CRC64;
MELDLGLSLS PHKSSKLGFN FDLNKHCAIE GAASCLGTEK LRFEATFGLG NVEENCYMPK
QRLFALNGQP NEEDEDPLES ESSIVYDDEE ENSEVVGWPP VKTCMIKYGS YHHRHIRNHH
HCPYHHRGRR ITAMNNNISN PTTATVGSSS SSSISSRSSM YVKVKMDGVA IARKVDIKLF
NSYESLTNSL ITMFTEYEDC DREDTNYTFT FQGKEGDWLL RGDVTWKIFA ESVHRISIIR
DRPCAYTRCL F